OBJETIVO: Avaliar o gasto energético na marcha em pacientes com mucopolissacaridose, utilizando uma metodologia simples e aplicável ao ambiente clínico. MÉTODOS: Realizou-se estudo transversal comparando-se o gasto energético da marcha de 19 pacientes portadores de mucopolissacaridose (Grupo MPS) com 19 indivíduos assintomáticos da comunidade (Grupo Comparação). O gasto energético foi mensurado em Cal por um relógio da marca Polar (modelo FT7) durante uma caminhada de 50 metros. Foram também avaliados idade, peso, altura, IMC, frequência cardíaca inicial, frequência cardíaca final, e tempo de marcha. RESULTADOS: O Grupo MPS teve gasto energético na marcha de 2,84 Cal(±1,01), versus 1,42 Cal(±0,51), sendo 100% maior que o Grupo Comparação; também em relação ao Grupo Comparação, o Grupo MPS teve frequência cardíaca inicial 22% maior, frequência cardíaca final 13% e tempo da caminhada 25% maiores. CONCLUSÕES: O gasto energético na marcha de pacientes com mucopolissacaridose é duas vezes mais alto em comparação com indivíduos assintomáticos e a metodologia usada para avaliação mostrou-se alternativa eficiente para o ambiente clínico convencional. Nível de Evidencia III, Estudo Transversal Comparativo.

OBJECTIVE: The aim of this study is to evaluate energy expenditure during gait in patients with mucopolysaccharidosis using a simple methodology applicable to the clinical setting. METHODS: A cross-sectional study was carried out comparing energy expenditure during gait in 19 patients with mucopolysaccharidosis (MPS Group) with 19 control individuals from the community (Comparison Group). Energy expenditure was measured in calories using a Polar telemetric watch (model FT7) in a 50-meters walking. Variables such as age, weight, height, BMI, initial hart rate, final hart rate, and gait time were recorded. RESULTS: MPS Group showed an expenditure during gait of 2.84 Cal (± 1.01) versus 1.42 Cal (± 0.51), being 100% higher than the Comparison Group; MPS Group had also increased initial hart rate (22% higher), final hart rate (13% higher), and time waking (13% faster). CONCLUSIONS: Energy expenditure during gait in MPS patients was twice higher compared to control individuals and the methodology for evaluation proved an efficient alternative to conventional clinical setting. Evidence Level III, Crosssectional Comparative Study.

Abstract The main objective of this work was to present the specification of an experimental firing test stand for liquid rocket engines (LRE) and develop a program for control and acquisition of data. It provides conditions to test rocket engines with thrust from 50 to 100 kgf. A methodology for laboratory work implementation using information technology, which will allow the automatic and remote functioning of the test stand, permits users to input the necessary data to conduct tests safely, achieve accurate measurements and obtain reliable results. The control of propellant mass flow rates by pressure regulators and other system valves, as well as the test stand data acquisition, are carried out automatically through LabVIEW commercial software. The test stand program is a readable, scalable and maintainable code. The test stand design and its development represent the state of art of experimental apparatus in LRE testing.

O presente trabalho foi conduzido com o objetivo de avaliar a eficácia de três técnicas de coloração nuclear de oócitos bovinos nos estágios de vesícula germinativa (VG), metáfase I (MI) e metáfase II (MII). Nestes três estágios de maturação nuclear, os oócitos, sem as células do cumulus, foram fixados em ácido acético:metanol (1:3) em lâminas (fixados em lâmina e corados com lacmóide; FLL) ou placa de petri (fixados em placa e corados com lacmóide; FPL) e corados com lacmóide a 1% em PBS (phosphate buffered saline). Os oócitos foram também distribuídos aleatoriamente a um terceiro grupo, no qual foram fixados em lâmina e corados com Giemsa (fixados em lâmina e corados com Giemsa; FLG). No estágio de VG, a percentagem de oócitos corretamente identificados no tratamento FLL (93,5%) foi significativamente maior do que nos tratamentos FPL (53,1%; χ²9,84; p-0,0017) e FLG (55,2%; χ2=9,03; p=0,0027). No entanto, a proporção de oócitos nos estágios de MI e MII precisamente corados com a técnica FLL (MI 41,7% e MII 41,9%) foram estatisticamente inferiores do que aquelas observadas nos tratamentos FPL (MI 90,0%, χ²13,25; p=0,0003, e MII 92.8%, χ2=12,45, p=0,0004) e FLG (MI 83,3%, χ2=10,69; p=0,0011, e MII 89,7%, χ²=12,24; p=0,0005). Assim sendo, os resultados demonstraram que a eficácia das técnicas de fixação e coloração dos oócitos bovinos depende do estágio de maturação nuclear que está em estudo.

This study was designed to determine the efficiency of three different technics for staining bovine oocyte in the germinal vesicle (GV), metafase I (MI) and metafase II (MII) stages. In these stages, oocytes without cumulus cells were fixed in acetic acid:methanol (1:3) either on slides (FLL) or in petri dishes (FPL) and stained with 1% of lacmoid in phosphate buffered saline (PBS). Also, the oocytes were randomized divided in a third treatment, in which they were fixed on slides immersed in acetic acid:methanol (1:3) and stained with Giemsa (FLG). In the GV stage, the percentage of oocytes accurately identified in the FLL (93.5%) was significantly higher than in the FPL (53.1%; χ2=9.84; p=0.0017) and FLG (55.2%; χ2=9.03; p=0.0027) treatment groups. However, the proportion of oocytes in the MI and MII stages correctly stained with FLL technic (MI 41.7% and MII 41.9%) was statistically lower than that observed in the FPL (MI 90.0%, χ²=13.25; p=0.0003, and MII 92.8%, χ²=12.45; p=0.0004) and FLG (MI 83.3%, χ²=10.69; p=0.0011, and MII 89.7%, χ²=12.24; p=0.0005) treatment groups. With these results, it can be established that the efficiency of the technic to fix and to stain bovine oocyte depends on the nuclear maturation stage to be investigated.