Abstract Spix's yellow-toothed cavies are rodents displaying high biological and farming potential. Knowledge of cavy reproductive aspects is paramount for satisfactory breeding. This study aimed to determine the gestation length and characterize the reproductive cycle phases of Spix's yellow-toothed cavies, also investigating potential male effects on these processes. The investigated animals were categorized into three groups: Gestational follow-up (G1), with a 5:1 female-to-male enclosure ratio; estrous cycle (G2), with a 5:1 female-to-male ratio with a male confined to a cage; and G3, consisting of five females and no male. Daily colpocytological examinations were performed, with the presence of spermatozoa on the microscopy slides indicative of copulation. G1 females were separated from the male immediately after copulation, with this being considered day "zero" of the pregnancy. G2 and G3 females were evaluated for two complete estrous cycles and qualitatively assessed through vaginal smears. The gestation length of the Spix's yellow-toothed cavies averaged 59 ± 2.24 days, with a continuous polyestrous cycle lasting 14.8 ± 0.73 days in G2 and 14.6 ± 0.75 days in G3. The proestrus phase was characterized by the dominance of parabasal cells, dyes, bacteria, and leukocytes; the estrus phase, by superficial cells with the predominance of anucleate cells with and without the presence of bacteria; the metestrus phase, by parabasal cells and numerous genuine cells, neutrophils, and bacteria; and the diestrus phase, mainly by basal, parabasal, and mutant cells, as well as high amounts of vaginal mucus, neutrophils, and bacteria. The presence of a male cavy significantly influenced diestrus duration, prolonging this phase, which is potentially attributed to female progesterone production effects.

Resumo O preá é um roedor com elevado potencial biológico e zootécnico a ser explorado, sendo o conhecimento sobre os aspectos reprodutivos fundamentais para que sua criação seja satisfatória. Objetivou-se determinar a duração da gestação em preás, e a caracterização das fases do ciclo reprodutivo, verificando, se existe influência da presença do macho neste processo. Os animais foram separados em três grupos: Acompanhamento gestacional (G1), 5:1, proporção de fêmea e macho no box; Ciclo estral, 5:1, com o macho preso em gaiola (G2) e cinco fêmeas em outro box sem o macho (G3). O exame colpocitológico ocorreu diariamente, identificado o espermatozoide na lâmina como cópula. As fêmeas do G1 foram separadas assim que copulavam e contadas como dia “zero” da gestação. As fêmeas do G2 e G3 foram avaliadas ao longo de dois ciclos estrais completos, avaliados qualitativamente pelo o esfregaço vaginal. O período de gestação em preás foi de 59±2,24 dias, com um ciclo poliéstrico contínuo, com duração de 14,8±0,73 dias no G2 e 14,6±0,75 dias no G3. O proestro caracterizou-se pelo predomínio de células parabasais, intermediárias, bactérias e leucócitos; o estro, células superficiais, com predomínio das anucleadas com presença ou não de bactérias; metaestro, células parabasais e grande quantidade de células intermediárias, neutrófilos e bactérias; diestro, predomínio de células basais, parabasais e intermediárias e grande quantidade de muco vaginal, neutrófilos e bactérias. A presença do macho influenciou significativamente a duração do diestro, tornando-se mais longa, fato que pode estar atrelado a influência sobre a produção de progesterona na fêmea.

Resumo Para o estudo foram utilizados 20 animais, que estavam em freezer no Centro de Multiplicação de Animais Silvestres (CEMAS/UFERSA). Os animais foram descongelados, a cavidade abdominal aberta e a aorta torácica canulada no sentido caudal para injeção de solução de látex Neoprene 650 corado de vermelho e, em seguida, fixados em solução de formaldeído a 10%, por um período mínimo de 48 horas, para que pudessem ser dissecados, fotografados e os resultados analisados. A artéria celíaca originou-se da aorta abdominal e emitiu as artérias gástrica esquerda, lienal e esplênica. A artéria gástrica esquerda emitiu de dois a três ramos à região da curvatura menor do estômago. A artéria lienal emitiu quatro ramos ao parênquima do baço, de seis a doze ramos pancreáticos e a artéria gastroepiplóica esquerda. A artéria hepática emitiu de um a dois ramos pancreáticos e bifurcou-se em um curto tronco formado pelas artérias gástrica direita e hepática própria e ainda na artéria gastroduodenal, que originava a pancreaticoduodenal cranial e gastroepiplóica direita. O comportamento de trifurcação da artéria celíaca do preá assemelha-se ao encontrado em roedores, como o nutria, a cutia, o hamster e os ratos, o que sugere que este seja o padrão para a ordem.

Abstract For this study we used 20 animals that were in the freezer at the Wild Animals Multiplication Center (CEMAS/UFERSA). The animals were thawed, the abdominal cavity was opened, and the thoracic aorta cannulated caudally for the injection of red-stained latex Neoprene 650, and then fixed in formaldehyde solution 10%, for a minimum of 48 hours. Then the animals were dissected, photographed, and the results analyzed. The celiac artery originated from the abdominal aorta and issued the left gastric, splenic, and hepatic arteries. The left gastric artery issued two to three branches to the lesser curvature of the stomach. The splenic artery emitted four branches to the parenchyma of the spleen, six to twelve pancreatic branches and the left gastroepiploic artery. The hepatic artery emitted from one to two pancreatic branches and forked in a short trunk formed by right gastric and own hepatic arteries and in the gastroduodenal artery, which originated the cranial pancreaticoduodenal and right gastroepiploic. The trifurcation behavior of the celiac artery of the yellow-toothed cavy is similar to that found in rodents such as nutria, agouti, hamsters and mice, suggesting that this is the standard for the order.

Glycosaminoglycans (GAGs) are heteropolysaccharides in mammalian tissue and consist of repeated disaccharide units with mono-sulfated or non-sulfated monosaccharides. GAGs are important components of the Extracellular Matrix (ECM) with several physiological roles, in the recognition, migration, adhesion, proliferation and differentiation processes. They are also important in angiogenesis, blood homeostasis, immune reactions, follicule development and also in the development of pathologies such as infertility, tumors and metastases. It has been shown that the profile of glycosaminoglycans in the uterine and placental tissues is highly variable throughout the reproductive cycle and during pregnancy. It may be directly related to their physiological or pathological functions in the tissue. The latter has recently triggered special clinical interest. Current review collaborates for a deeper knowledge on the profile and importance of GAGs in uterine and placental tissues throughout the reproductive cycle and pregnancy. It also covers information on the involvement of these molecules in pathological processes.

The objective of this review was to describe sexual differentiation events in mammals, relating them to biosynthesis of sexual steroid hormones and their mechanisms of action. Cholesterol is the precursor of sexual steroid hormone biosynthesis via action of several enzymes converting these hormones. Progestagens hormones serve as substrate for the production of androgens, which in turn serve as substrate for estrogen hormones. These hormones are responsible for sexual differentiation and reproductive cycles of mammals. Sexual differentiation process comprises determining the sexual chromosomes XX or XY + SRY and other genes linked to them, differentiation of gonads in testis or ovary, differentiation of internal and external male or female genital organs from undifferentiated anatomical structures present in the embryo, which is dependent on the presence or absence of testes and the production of anti-Müllerian hormone and testosterone; and secondary sexual differentiation, which is the response of various tissues to hormones produced by the gonads, interacting with genes linked to sexual chromosomes to increase or decrease the differences in sexual phenotype. However, some differences between the sexes and some anomalies of sexual differentiation are not explained only by these sexual hormonal effects, but also by the effect of genes encoded in sexual chromosomes.

O preá do semiárido nordestino (Galea spixii Wagler, 1831) é um roedor pertencente à família Caviidae. Pouca literatura é encontrada sobre essa espécie em relação a sua morfologia e seu comportamento ambiental e reprodutivo. Com o objetivo de entender a morfologia geral, em foco, na inervação do membro pélvico dessa espécie, neste trabalho, foi explorado o nervo isquiático, o qual é o maior de todos os nervos do organismo. Foram utilizados 10 preás (cinco machos e cinco fêmeas) que vieram a óbito por causas naturais, oriundos do Centro de Multiplicação de Animais Silvestres da Universidade Federal Rural do Semiárido (CEMAS/UFERSA). Os animais foram fixados após o óbito em solução aquosa de formaldeído 10% e, após 48 horas de imersão nessa solução, foram dissecados para exposição do nervo isquiático. Dessa forma, os dados obtidos foram compilados em tabelas e expressos em desenhos esquemáticos e fotografias. Os pares de nervos isquiáticos originaram-se de raízes ventrais de L6L7S1 (70%) e de L7S1S2 (30%) e distribuíram-se pelos músculos glúteo profundo, bíceps femural, semitendinoso e semimembranoso.

The Galea of the semi-arid northeast (Galea spixii Wagler, 1831) is a rodent belonging to the family Caviidae. Little literature is found on this species in relation to morphology, environmental and reproductive behavior. With a view to understanding the general morphology, particularly, in the innervation of the pelvic limb of this species, ponder the sciatic nerve, which is the largest of all the nerves of the body. 10 cavies were used (five males, five females) that they had died of natural causes, originating from breeders scientific, legally licensed by IBAMA, the UFRSA, Mossoró, RN. The animals were fixed after the death in an aqueous solution of formaldehyde 10% and after 48 hours of immersion in it, were dissected to expose the sciatic nerve. Thus, the data were compiled in tables and expressed in drawings and photographs. The pairs of sciatic nerves were derived from ventral roots L6L7S1 (70%) and L7S1S2 (30%) and were distributed by the deep gluteal muscles, biceps femural, semitendinosus and semimembranosus muscles.