RESUMO Objetivou-se caracterizar o cenário da aquisição dos alimentos provenientes da Agricultura Familiar (AF) para o Programa Nacional de Alimentação Escolar (PNAE) nos municípios do Rio Grande do Norte. Pesquisa avaliativa com delineamento ecológico, utilizando dados do Centro Colaborador em Alimentação e Nutrição Escolar da Universidade Federal do Rio Grande do Norte em 2017 e 2018. Avaliaram-se 31 municípios, analisando o percentual de aquisição de alimentos da AF para a alimentação escolar, além das possíveis dificuldades nesse processo, utilizando variáveis relacionadas com governança e gestão, estabilidade e disponibilidade de alimentos. Dos municípios avaliados, mais de 50% adquiriram alimentos da AF no ano anterior e afirmaram a compra no ano atual. Todos relataram empregar o modelo de gestão centralizada, 29% declararam aquisição menor que 30%, 9,7%, aquisição acima de 30%, e 61,3% não souberam informar. Quanto às principais dificuldades relacionadas com governança e gestão, a articulação intersetorial e o edital de chamada pública ganharam destaque. Sobre a estabilidade de alimentos, destacaram-se as condições higienicossanitárias necessárias, e em relação à disponibilidade de alimentos, a aquisição de alimentos orgânicos mostrou-se como uma dificuldade elencada por 80,6% dos municípios.

ABSTRACT The objective was to characterize the scenario of acquisition of food from Family Farming (AF) for the National School Feeding Program (PNAE) in the municipalities of Rio Grande do Norte. An evaluative research with an ecological design, using data from the Collaborating Center for School Food and Nutrition of the Federal University of Rio Grande do Norte in 2017 and 2018. 31 municipalities were evaluated, analyzing the percentage of food purchases from AF for school meals, in addition to of the possible difficulties in this process, using variables related to governance and management, stability and availability of food. Of the evaluated municipalities, more than 50% purchased food from the AF in the previous year and confirmed the purchase in the current year. All reported using the centralized management model, 29% declared acquisition less than 30%, 9.7%, acquisition above 30%, and 61.3% were unable to inform. As for the main difficulties related to governance and management, intersectorial articulation and the public call notice were highlighted. Regarding food stability, the necessary hygienic-sanitary conditions were highlighted, and in relation to food availability, the purchase of organic food was shown to be a difficulty listed by 80.6% of the municipalities.

RESUMO Objetivou-se caracterizar o cenário da aquisição dos alimentos provenientes da Agricultura Familiar (AF) para o Programa Nacional de Alimentação Escolar (PNAE) nos municípios do Rio Grande do Norte. Pesquisa avaliativa com delineamento ecológico, utilizando dados do Centro Colaborador em Alimentação e Nutrição Escolar da Universidade Federal do Rio Grande do Norte em 2017 e 2018. Avaliaram-se 31 municípios, analisando o percentual de aquisição de alimentos da AF para a alimentação escolar, além das possíveis dificuldades nesse processo, utilizando variáveis relacionadas com governança e gestão, estabilidade e disponibilidade de alimentos. Dos municípios avaliados, mais de 50% adquiriram alimentos da AF no ano anterior e afirmaram a compra no ano atual. Todos relataram empregar o modelo de gestão centralizada, 29% declararam aquisição menor que 30%, 9,7%, aquisição acima de 30%, e 61,3% não souberam informar. Quanto às principais dificuldades relacionadas com governança e gestão, a articulação intersetorial e o edital de chamada pública ganharam destaque. Sobre a estabilidade de alimentos, destacaram-se as condições higienicossanitárias necessárias, e em relação à disponibilidade de alimentos, a aquisição de alimentos orgânicos mostrou-se como uma dificuldade elencada por 80,6% dos municípios.

ABSTRACT The objective was to characterize the scenario of acquisition of food from Family Farming (AF) for the National School Feeding Program (PNAE) in the municipalities of Rio Grande do Norte. An evaluative research with an ecological design, using data from the Collaborating Center for School Food and Nutrition of the Federal University of Rio Grande do Norte in 2017 and 2018. 31 municipalities were evaluated, analyzing the percentage of food purchases from AF for school meals, in addition to of the possible difficulties in this process, using variables related to governance and management, stability and availability of food. Of the evaluated municipalities, more than 50% purchased food from the AF in the previous year and confirmed the purchase in the current year. All reported using the centralized management model, 29% declared acquisition less than 30%, 9.7%, acquisition above 30%, and 61.3% were unable to inform. As for the main difficulties related to governance and management, intersectorial articulation and the public call notice were highlighted. Regarding food stability, the necessary hygienic-sanitary conditions were highlighted, and in relation to food availability, the purchase of organic food was shown to be a difficulty listed by 80.6% of the municipalities.

The peanut is an oleaginous plant of high nutritional value, a source of protein and a trypsin inhibitor. Trypsin inhibitors are proteins present in the vegetable kingdom, considered anti-nutritional factors for animals. However, there have been several recent reports about their heterologous and beneficial effects on human health. These important effects have been the focus of studies investigating these inhibitors in foods. The aim of the present study was to isolate and determine the estimated molecular mass and specific inhibitory activity, for trypsin in the Japanese peanut, peanut butter, and peanut nougat using the techniques of precipitation with ammonium sulfate and affinity chromatography on trypsin - Sepharose CNBr 4B. The techniques used in this study were efficient for isolating the protein inhibitors with antitryptic specific activity of 694 UI mg-1, 823 UI mg-1 and 108 UI mg-1 for the Japanese peanut, peanut nougat, and peanut butter, respectively. The techniques featured high selectivity of the adsorbent, with consequent efficiency in isolation, given the low amount of dosed proteins and specific antitryptic activity presented by the products studied. The various health-related benefits show the importance of detecting and isolating efficient trypsin inhibitors in foods, taking into account the health claims attributed to the vegetable and its high consumption by humans.