ABSTRACT Oxisols are important soils that have been converted from native vegetation to croplands, and can affect soil biological properties such as microbial biomass and enzyme activity. Thus, the aim of this study was to evaluate the changes on soil microbial biomass and enzyme activity when native vegetation (NV) was converted to cropland (CL), such as maize or sugarcane in six oxisols from São Paulo state, Brazil. Soil microbial biomass C (MBC) and N (MBN), and the activity of arylsulphatase, dehydrogenase and fluorescein diacetate hydrolysis (FDA) were assessed in samples collected at 0-0.20 m. In general, MBC was higher under NV than CL (about + 40%), while MBN and FDA did not show a consistent pattern between NV and CL. All soils showed higher values of arylsulfatase (increased from 101 to 717%) and dehydrogenase (increased 15 to 220%) under NV than CL. In conclusion, soil microbial biomass C is usually higher under native vegetation than cropland. Arylsulphatase and dehydrogenase were the attributes that presented better differentiation between native and cropped soils.

The objective of the present study was to evaluate the characteristics of the sward canopy of Marandu grass during the rainy season, the wet-to-dry transition and the dry seasons, between March and September 2004, under intermittent grazing, and to correlate those characteristics with the performance of crossbred heifers receiving mineral supplements ad libitum or protein supplements. The experiment consisted of a randomized block design with three blocks (set of 13 paddocks), each containing five crossbred heifers per experimental unit, totaling 15 replicates. The heifers were given protein supplements daily in individual stalls and received an average 4 g/kg/day of the supplement during the rainy season and 5 g/kg/day during the dry season. Their weight gain was assessed monthly. The pasture structure was assessed through destructive sampling, and the bromatological composition of esophageal extrusa samples was also assessed. Analysis of variance was used to assess performance, and regression analysis was used to evaluate the sward canopy characteristics in relation to the months of the year. A cluster procedure was used to determine the similarity between the months of the year under assessment. Two different groups were formed for pasture evaluation: one group including the months of March to July and another group including the months of August and September. The first group exhibited a better canopy structure than the second group. This fact was corroborated by the animal performance, which was lower during the months of the second group. Low-intake protein supplementation was effective in increasing the performance of the grazing heifers. Pasture structure is critical for animal performance in a grazing environment, regardless of the type of supplementation.

O objetivo deste trabalho foi avaliar a concentração de nitrogênio em plantas de milho e ao longo do perfil de um Latossolo Vermelho eutroférrico submetido a doses de lodo de esgoto e fertilizante mineral. O experimento foi instalado em delineamento experimental em blocos casualizados com 4 tratamentos (0,0; 55,0; 110,0 e 167,5 Mg.ha-1 de lodo de esgoto em doses acumuladas) e 5 repetições para um período de avaliação de 1 ano. A amostragem de solo foi realizada aos 60 dias após a emergência (d.a.e.) das plantas nas profundidades 0,0-0,1, 0,1-0,2, 0,2-0,4, 0,4-0,6, 0,6-0,8 e 0,8-1,0 m. Também aos 60, 80 e 128 d.a.e. foram coletadas, respectivamente, folha diagnose, planta inteira e grão. As maiores doses de lodo de esgoto proporcionaram maiores quantidades de nitrogênio no solo. O nitrogênio do solo, após a profundidade de 0,6 m, não diferiu entre as camadas avaliadas. Quanto maior a dose de lodo de esgoto, menor a proporção de nitrogênio em profundidade.

The aim of this study was to evaluate the concentration of nitrogen in corn plants and along the profile of an Oxisol subjected to doses of sewage sludge and mineral fertilizer. The experiment was conducted in randomized complete block design with 4 treatments (0.0; 55.0; 110.0 and 167.5 Mg.ha-1 of sewage sludge accumulated doses) and 5 reps for a trial period of 1 year. Soil sampling was performed 60 days after emergence (d.a.e.) plants at depths 0-0.1, 0.1-0.2, 0.2-0.4, 0.4-0.6, 0.6-0.8 and 0.8-1.0 m. Also at 60, 80 and 128 d.a.e. were collected, respectively, diagnosis leaf, whole plant and grain. Higher doses of sewage sludge provided higher amounts of nitrogen in the soil. The soil nitrogen, after the depth of 0.6 m, did not differ between the layers evaluated. The higher the dose of sewage sludge, the lower the proportion of nitrogen in depth.

Avaliou-se o efeito de níveis de suplementação no desempenho de novilhos de três grupos genéticos (Nelore x Red Angus (NR), Nelore (NL) e mestiço Holandês (MH)), em três períodos experimentais. O desempenho do primeiro período foi analisado em DBC, composto por três tratamentos (NR, NL e MH) e três blocos. No segundo período, usou-se DBC em esquema de análise fatorial 3 x 3, com três blocos. O fator A determinou o grupo (NR, NL e MH) e o fator B, níveis de suplementação (0,2; 0,6 e 1% PV). No terceiro período, usou-se DIC em esquema de análise fatorial 2 x 3. O fator A determinou o grupo (NL e MH) e o fator B, três níveis de suplementação. No início, foi verificado maior ganho de peso (GP) no NR (0,79 kg dia-1) comparado ao MH e N (0,73 e 0,61 kg dia-1). O consumo de concentrado variou no NL. No segundo período, o NR apresentou maior GP (0,91 kg dia-1) comparado ao NL (0,75 kg dia-1) e MH (0,76 kg dia-1), sendo influenciados pelos níveis de suplementação. No terceiro período, MH apresentou maior GP (0,89 kg dia-1). Não houve diferença entre os animais com 0,8 e 1,2% do PV, com GP de 0,82 e 0,92 kg dia-1.

This trial was carried out to study the effects of supplementation level on performance of steers from different genetic groups (Nellore x Red Angus (NR), Nellore (NL) and Holstein crossbred (MH), and three experimental periods. The performance data for first period were analyzed according to randomized block (RB) design, with 3 treatments ((NR, NL and MH)) and 3 blocks. In second period, the RB design used with 3 x 3 factorial design, and 3 blocks. Factor A determined the group (NL and MH) and factor B the supplementation levels (0.2, 0.6 and 1% LW). In third period, the data were evaluated according to completely randomized design, with 2 x 3 factorial design. Factor A determined the group and factor B, three levels of supplementation. In the first, higher weight gain (p < 0.05) was observed for NR (0.79 kg day-1) compared to MH and NL (0.73 and 0.61 kg day-1). The concentrate intake varied for N. NR showed higher (p < 0.05) WG (0.91 kg day-1) compared to NL (0.75 kg day-1) and MH (0.76 kg day-1). The levels of supplements influenced (p < 0.05) WG (0.68, 0.81 and 0.92 referring to levels of 0.2, 0.6 and 1.0% LW). In third period, MH presented greater WG < 0.05) (0.895 kg day-1). No difference was verified on animals with 0.8 and 1.2% LW, with WG of 0.92 and 0.82 kg day-1.

Este trabalho foi realizado na Faculdade de Ciências Agrárias Veterinárias - Unesp - Jaboticabal e foi conduzido, com o objetivo de avaliar a influência do método de amostragem do pasto sobre a degradabilidade in situ da matéria seca e da fração fibrosa de capim Marandu, colhido no período seco dos anos de 2003 e 2005. O experimento foi instalado em delineamento de blocos casualizados com parcela subdividida, com três repetições, representadas pelos piquetes amostrados. Nas parcelas, foram avaliados cinco métodos de amostragens de forragem (método do quadrado metálico; método de avaliação através de extrusa de bovino da raça Nelore; método de avaliação por meio de extrusa de bovino Cruzado (Red Angus x Nelore); método de avaliação por meio do pastejo, simulando bovino da raça Nelore; método de avaliação por meio do pastejo, simulando bovino Cruzado (Red Angus x Nelore) e as subparcelas foram constituídas pelos anos de amostragem, 2003 e 2005. Foram determinadas as frações da cinética ruminal: solúvel "A"; insolúvel potencialmente degradável "B"; taxa de degradação "Kd"; degradação potencial (DP) e fração não degradável "C" da MS e degradação potencial (DP), fração não degradável "C" e taxa de degradação "Kd" da FDN e da FDA. De acordo com os resultados obtidos, observou-se que o método do quadrado metálico subestimou as características da degradação do capim. Os métodos do pastejo simulado se assemelharam muito ao das extrusas, no entanto, a prática do simulador é que assegurou a amostragem eficiente, conforme foi constatado pelos dados obtidos no ano de 2003 e 2005.

This experiment was carried out at the Faculdade de Ciências Agrárias Veterinárias - Unesp - Jaboticabal during dry season, to evaluate the effects of sampling methods on the Marandu grass dry matter and cell wall in situ degradability. The data were analyzed according to a randomized block design with three replications (paddock), in a split plot scheme. Five sampling methods were evaluated on the parcels: metallic square method; Nellore steers extrusa, Red Angus x Nellore crossbreeding steers extrusa, simulated grazing of the Nellore, simulated grazing of Red Angus x Nellore crossbreeding, and two years (2003 and 2005) on the subparcels. The following rumen kinetic fractions were determined: soluble "A", degradable potential "B", degradability rate "Kd", the potential degradability (DP) and no degradable fraction "C" of the FDN, and FDA. According to the results obtained, the metallic square method underestimated the characteristics of the forage degradation. The results of the simulated grazing method resembled the extrusas method; however, it was the simulator practice that assured efficient sampling, as shown by evidence from data obtained between 2003 and 2005.