Abstract CD20 is a transmembrane receptor, highly expressed in more than 90% of lymphocytes. Our research aims to probe CD20 signaling pathway in the proliferation, invasion and expression of related proteins IDO/AHR. OCI-Ly3 cells were with CD20 or inhibitor rituximab, and apoptosis, cell cycle, proliferation index (PI) were detected by flow cytometry. Cell invasion and migration detection using Transwell. Western blot detection of IDO/AHR levels. Comparing to blank control group, the percentage of S in the cell cycle of the cells treated with CD20 decreased significantly, and the PI increased significantly. With the extension of the culture time, the S ratio and PI further increased significantly, the cell motility and infiltration ability were significantly enhanced, and the IDO/AHR protein level increased. Inhibition of CD20 signaling pathway (rituximab) can hamper the cells, reduce cell invasion or migration, and the protein expression of IDO/AHR in cells is significantly reduced. Correlation analysis showed that the expression of CD20 and IDO/AHR showed a negative correlation. CD20 take part in the occurrence and development of lymphoma. Its expression mainly regulates the occurrence and development of lymphoma by affecting the proliferation and invasion of lymphoma cells. Our results show that CD20 can mediate IDO/AHR and affect lymphoma. Mechanism research.

Background Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to analysis the sequence characterization of banana ASR1, and identify its expression patterns and function under salt stress using quantitative real-time PCR (qPCR) and overexpression in Arabidopsis. The purpose was to evaluate the role of banana ASR1 to salt stress tolerance employed by plants. Results A full-length cDNA isolated from banana fruit was named MaASR1, and it had a 432 bp open reading frame (ORF) encoding 143 amino acids. MaASR1 was preferential expression in roots and leaves compared to low expression in fruits, rhizomes and flowers. Under salt stress, the expression of MaASR1 quickly increased and highest expression level was detected in roots and leaves at 4 h, and then gradually decreased. These results suggested that MaASR1 expression was induced under salt stress. MaASR1 protein was localized in the nucleus and plasma membrane. MaASR1 was transformed to Arabidopsis and verified by southern and northern analysis, transgenic lines L14 and L38 integrated one and two copies of MaASR1, respectively, while overexpression in transgenic lines provided evidence for the role of MaASR1 to salt stress tolerance. Conclusions This study demonstrated that overexpression of MaASR1 in Arabidopsis confers salt stress tolerance by reducing the expression of ABA/stress-responsive genes, but does not affect the expression of the ABA-independent pathway and biosynthesis pathway genes.