Abstract Background: Previous studies have shown that long noncoding RNA (IncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this IncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the effect of LINC00483 on gastric cancer development and explore the potential regulatory network of LINC00483/microRNA (miR)-490-3p/mitogen-activated protein kinase 1 (MAPK1). Methods: Thirty patients with gastric cancer were recruited for tissues collection. The expression levels of LINC00483, miR-490-3p and MAPK1 were detected by quantitative real-time polymerase chain reaction or western blot. Cell viability, apoptosis, migration and invasion were determined by MTT, flow cytometry, transwell assays and western blot, respectively. The target association between miR-490-3p and LINC00483 or MAPK1 was confirmed by luciferase reporter assay. Xenograft model was established to assess the function of LINC00483 in vivo. Results: LINC00483 and MAPK1 levels were increased in gastric cancer tissues and cells. Knockdown of LINC00483 or MAPK1 inhibited cells viability, migration and invasion but promoted apoptosis in gastric cancer cells. Moreover, MAPK1 overexpression attenuated the effect of LINC00483 knockdown on gastric cancer development. LINC00483 could increase MAPK1 expression by competitively sponging miR-490-3p. miR-490-3p overexpression suppressed gastric cancer development, which was abated by introduction of LINC00483. Besides, inhibition of LINC00483 decreased xenograft tumor growth by regulating miR-490-3p/MAPK1 axis. Conclusion: Knockdown of LINC00483 inhibited gastric cancer development in vitro and in vivo by increasing miR- 490-3p and decreasing MAPK1, elucidating a novel mechanism for understanding the development of gastric cancer.

2-Tosiloxi-1,3-propanodiol (TPD), um potencial precursor para produção de 1,3-propanodiol (1,3-PD), é produzido através da tosilação do glicerol com a ajuda de uma técnica de proteção de grupos. Neste trabalho, discutiu-se a hidrogenólise do TPD em 1,3-PD sobre catalisador de Raney Ni através de diferentes parâmetros de reação para otimizar as condições de reação para formação seletiva do 1,3-PD. O mecanismo de hidrogenólise do TPD e reações laterais também foram confirmados pela técnica de cromatografia em fase gasosa acoplada à espectrometria de massas (GC-MS).

2-Tosyloxy-1,3-propanediol (TPD), a potential precursor for 1,3-propanediol (1,3-PD) production, is produced by the tosylation of glycerol with the help of protecting group techniques. In this work, the hydrogenolysis of TPD into 1,3-PD over Raney Ni catalyst is discussed at different reaction parameters to optimize the reaction conditions for selective formation of 1,3-PD. The mechanisms of the hydrogenolysis of TPD and the side reactions were also confirmed by gas chromatography-mass spectrometry (GC-MS) technique.

Cinco derivados naturais [ácido ferúlico (FA), polifenol de chá lipidio-solúvel (LTP), polifenol de chá solúvel em água (WTP), sesamol (SE) e ácido cafeico (CA)] foram avaliados como aditivos antioxidantes para melhorar a estabilidade da oxidação de biodiesel de óleo de soja (SBO-BD). Verificou-se que WTP, LTP, SE e CA podem melhorar significativamente a estabilidade de SBO-BD. CA apresentou o melhor desempenho no intervalo de concentração de 500 a 1500 ppm, enquanto que WTP mostrou atividade mais elevada a uma concentração de 200 ppm. Para comparação, o efeito estabilizador de três antioxidantes sintéticos [α-tocoferol (α-T), butilhidroxitolueno (BHT) e butilhidroxianisol (BHA)] foi avaliado simultaneamente. Os resultados mostraram que o efeito de alguns aditivos naturais (WTP, LTP, SE e CA) foi melhor que de aditivos sintéticos (α-T, BHT e BHA) na estabilidade da oxidação de SOB-BD. Uma explicação possível para os efeitos diferentes entre os antioxidantes testados foi elaborada levando em conta sua estrutura molecular.

Five natural derivatives [ferulic acid (FA), lipid-soluble tea polyphenol (LTP), water-soluble tea polyphenol (WTP), sesamol (SE) and caffeic acid (CA)] were evaluated as antioxidative additives for enhancing the oxidation stability of soybean oil based biodiesel (SBO-BD). It was found that WTP, LTP, SE and CA could significantly improve the oxidation stability of SBO-BD. CA displayed the best performance in the concentration range from 500 to 1500 ppm, whereas WTP displayed the highest activeness at a concentration of 2000 ppm. For comparison, the stabilizing effect of three synthetic anti-oxidants [α-tocopherol (α-T), butylhydroxytoluene (BHT) and butylhydroxyanisol (BHA)] was simultaneously evaluated. The results showed that the effect of some natural additives (WTP, LTP, SE and CA) was better than that of synthetic additives (α-T, BHT and BHA) on the oxidation stability of SBO-BD. A possible explanation for the different effects among the tested antioxidants was given based on their molecular structures.