Abstract The potential of long noncoding RNAs (lncRNAs) as symptomatic and prognostic biomarkers in oncotherapy for hepatic carcinoma has been revealed. However, the specific mechanisms of lncRNA-regulated tumor progression and metastasis are not completely known. The RNA and miRNA sequencing data of liver cancer in The Cancer Genome Atlas database were analyzed. A competing endogenous RNA (ceRNA) was constructed, and its effects on the progression and metastasis of tumors and the survival rates of patients with tumor were evaluated. Differentially expressed mRNAs, miRNAs, and lncRNAs were identified. A liver cancer-associated ceRNA network, namely, FAM224A/hsa-mir-139/RAD54B, was then established. FAM224A/hsa-mir-139/RAD54B assumed a significant part in liver malignancy movement and metastasis. A series of cell experiments was conducted to prove the importance of ceRNAs, and the binding between RNAs was validated through a dual-luciferase reporter assay. The FAM224A/hsa-mir-139/RAD54B network in liver cancer was identified. FAM224A may be an important prognostic factor and treatment target for liver cancer.

Abstract: Background: Serum amyloid A is an acute-phase protein. There is no available data regarding serum amyloid A levels in patients with acute (AU) and chronic urticaria (CU). Objective: To investigate the association between serum amyloid A and urticaria. Methods: This was a case-control study of 81 patients who visited our Hospital between June and December 2016 with a diagnosis of urticaria. Eighty healthy controls (HC) who visited for routine health examination and physical checkups were recruited. Serum amyloid A and C-reactive protein levels were measured by automated methods. Results: Serum amyloid A and C-reactive protein levels were significantly higher in AU (Serum amyloid A: 207.1 (6.7-439.0) mg/L; C-reactive protein: 16.0 (0.2-90.0) mg/L) and CU (Serum amyloid A: 6.5 (2.5-35.8) mg/L; C-reactive protein: 1.0 (0.1-16.0) mg/L) compared with HC (Serum amyloid A: 5.04 (2.0-9.1) mg/L; C-reactive protein: 1.2 (0.1-5.6) mg/L), and in AU compared with CU (all P<0.05). There were no differences between the CU and HC group. In CU, Serum amyloid A levels in those with moderate/severe urticaria (median, 16.4 (9.7-35.8) mg/L) were higher than in those with mild urticaria (median, 5.7 (2.5-9.5) mg/L) and HC (all P<0.05). Serum amyloid A and C-reactive protein levels exceeded the normal lab range in 90.7% and 72.1% patients with AU compared with 28.9% and 13.2% patients with CU, respectively. Significant positive correlations were found between serum amyloid A and C-reactive protein (r = 0.562, P < 0.001). Study limitations: There was no comparison between active disease and remission. Conclusion: There was an association between serum amyloid A levels and urticaria. Higher serum amyloid A levels were associated with AU and more severe CU. Serum amyloid A may help to identify CU patients earlier.

Abstract: Introduction and aim. Hyponatremia is common in patients with decompensated cirrhosis and is associated with increased mortality. Tolvaptan, a vasopressor V2 receptor antagonist, can increase free wáter excretion, but its efficacy and safety in cirrhotic patients remain unclear. Material and methods. We studied the usage and safety of tolvaptan in cirrhotic patients in a real-life, non-randomized, multicenter prospective cohort study. Forty-nine cirrhotic patients with hyponatremia were treated with tolvaptan 15 mg daily, and 48 patients not treated with tolvaptan in the same period served as controls. Improvement in serum sodium level was defined as an increase in serum sodium from < 125 to ≥ 125 mmol/L or from 125-134 to ≥ 135 mmol/L on day 7. Results. Twenty-three (47%) patients in the tolvaptan group and 17 (35%) in the control group had normal serum sodium on day 7 (p = 0.25). Serum sodium improved in 30 (61%) patients in the tolvaptan group and 17 (35%) patients in the control group (p = 0.011). Adverse events occurred in 46-47% of patients in both groups, and tolvaptan was not associated with worsened liver function. No patient with normal serum sodium on day 7 died within 30 days of treatment, whereas 16% of those with persistent hyponatremia died (p = 0.0019). Conclusion. In conclusion, short-term tolvaptan treatment is safe and can improve serum sodium level in cirrhotic patients with hyponatremia. Normalization of serum sodium level is associated with better survival.

Objective: the purpose of this study is to assess the current status of disordered eating attitudes and its related factors among University students in Anhui province, and to provide a basis for health intervention. Methods: this is a cross-sectional study. The University students admitted to the routine health exams were included in current study, and completed self-administered questionnaires which consist of general information and the Eating Attitudes Test-26 (EAT-26). An EAT-26 score of 20 or higher indicated that a person has disordered eating attitudes. Data were analyzed using the SPSS13.0 software. Results: a total of 1 328 subjects (469 male and 859 female), aged 16-24 years from a university in Wuhu were enrolled in this study. In our survey, the proportion of disordered eating attitudes among male, female, total students were 5.3%, 4.0% and 4.5%, respectively. The proportion of disordered eating attitudes among the students whose family annual income < 10000 RMB, 10 000-30 000 RMB, 30 000-60 000 RMB and > 60 000 were 4.2%, 3.9%, 4.3% and 6.9%, respectively. An interesting finding was that the female students are more likely to have disordered eating attitudes if their parents have more education. Conclusions: our research reveals that the current status of disordered eating attitudes in Anhui province is relatively low. Parents' education level may be related to eating attitudes among University students. It is essential to increase awareness and understanding of eating disorders and its associated risk factors in University students whose parents have higher education.

Objetivo: el proposito de este estudio es evaluar la situación actual de trastornos alimenticios y sus factores relacionados entre los estudiantes universitarios de la provincia de Anhui, y proporcionar una base para la intervención sanitaria. Métodos: este es un estudio transversal. En el estudio se incluyeron estudiantes universitarios a los que se les realizaron exámenes de salud rutinarios y que completaron cuestionarios que consisten en información general y sobre las actitudes alimentarias: test-26 (EAT-26). Según el test EAT-26, una puntuación de 20 o mayor indica que el sujeto presenta trastornos alimentarios. Los datos fueron analizados mediante el software de SPSS13.0. Resultados: en el estudio se incluyeron un total de 1.328 estudiantes de una universidad de Wuhu (469 hombres y 859 mujeres). En nuestro estudio, la proporción de trastornos alimentarios de hombres y mujeres y el total de estudiantes fueron 5,3%, 4,0%, 4,5%, respectivamente. La proporción de trastornos alimentarios entre los estudiantes cuya familia poseía unos ingresos anuales < 10.000 RMB, 10.000-30.000 RMB, 30.000-60.000 RMB y > 60.000 fueron 4,2%, un 3,9%, 4.3% y 6,9%, respectivamente. Un hallazgo interesante es que los estudiantes son más propensos a padecer trastornos alimentarios si sus padres tienen más educación. Conclusiones: nuestra investigación revela que la situación actual respecto a trastornos alimentarios en la provincia de Anhui es relativamente baja. El nivel de educación de los padres puede ser relacionado con las actitudes de los estudiantes universitarios. Es esencial aumentar la conciencia y la comprensión de los trastornos de la alimentación y sus factores de riesgo asociados con respecto a los estudiantes universitarios cuyos padres tienen una educación superior.

Background: Overweight and obesity are epidemic worldwide. Our previous study found that the prevalence of overweight and obesity in primary students is high. Objective: we sought to estimate whether the prevalence of overweight and obesity among university students is still high in China. Methods: A cross-sectional study was designed to collect the routine health screening data for university students in 2013. The height and weight of students were measured, and BMI was calculated with height and weight, we estimated the underweight and obesity prevalence of university students using by two references [Working Group on Obesity references in China (2004) and World Health Organization (WHO) criteria (2000)]. Results: Depending on the China references used, the overall prevalence of underweight, overweight (including obesity) and obesity in male university students were 14.2%, 14.7% and 4.2%, respectively; in female university students were 27.5%, 4.4% and 0.6%, respectively. Depending on the WHO references used, the overall prevalence of underweight, overweight (including obesity) and obesity in male university students were 14.2%, 11.5% and 2.5%, respectively, in female university students were 27.5%, 2.4% and 0.3%, respectively. An interesting observation made was that the underweight prevalence of university students has an increasing trend, especially in female. Conclusion: The study showed that the prevalence of underweight in university students, especially in female students has become a critical health issue. Related department of school and government should pay more attention to student's physical health.

Antecedentes: El sobrepeso y la obesidad son epidemia en todo el mundo.Nuestro estudio anterior halló que la prevalencia de sobrepeso y obesidad en estudiantes de primaria es alto. Objetivo: Hemos tratado de estimar si la prevalencia del sobrepeso y la obesidad entre los estudiantes universitarios es todavía alta en China. Métodos: Un estudio transversal fue diseñado para recoger los exámenes de salud de rutina datos para estudiantes universitarios en 2013.La altura y el peso de los estudiantes fueron medidos, y el IMC se calcula con la altura y el peso, así como para estimar la prevalencia de insuficiencia ponderal y obesidad de la Universidad los estudiantes utilizando dos referencias [Grupo de trabajo sobre la obesidad referencias en China (2004) y la Organización Mundial de la salud (OMS) criterios (2000)]. Resultados: Dependiendo de la China referencias utilizadas, la prevalencia global de bajo peso, sobrepeso (obesidad) y la obesidad en estudiantes universitarios masculinos fueron 14,2%, el 14,7% y 4,2%, respectivamente; en mujeres estudiantes universitarios fueron el 27,5%, 4,4% y 0,6%, respectivamente. Dependiendo de los que referencias utilizadas, la prevalencia global de bajo peso, sobrepeso (obesidad) y la obesidad en estudiantes universitarios masculinos fueron 14,2%, 11,5% y 2,5%, respectivamente, en mujeres estudiantes universitarios fueron el 27,5%, 2,4% y 0,3%, respectivamente.Una observación interesante es que la prevalencia de insuficiencia ponderal de los estudiantes universitarios tiene una tendencia al aumento, sobre todo en mujeres. Conclusión: El estudio demostró que la prevalencia de la insuficiencia ponderal en estudiantes universitarios, especialmente en mujeres estudiantes se ha convertido en un problema de salud crítico.Related Departamento de la escuela y el Gobierno deben prestar más atención a la salud física del alumno.

Background: In recent years, obesity was a major public health problem in many countries. It was estimated that 8% of their children are obese. However, little is known about the overall prevalence of obesity among secondary students in China, the aim of this study was to evaluate the overall obesity prevalence of student from Chinese secondary school. Methods: Publications from 2009 to 2014 on the obesity prevalence among secondary school students in China were retrieved from PubMed, online Chinese periodical full-text databases of VIP, CNKI and Wan fang. Meta Analyst was used analyze the total rates of obesity for Chinese secondary school. Results: After evaluation of the quality of the articles, 32 papers were finally included in our study, and the total sample sizes on the obesity investigation were 218317 (107631 male and 110686 female), in which 27455(14865 male and 12590 female) were obesity. Meta-analyst findings showed that the pooled prevalence of obesity in secondary school students are 8.4 %( 95% CI: 6.2%- 11.3%) and 4.8 %( 95% CI: 3.2%-7.2%) for boy and girl respectively. Conclusion: Our results suggest that school and government related department should pay more attention to the obesity among secondary school students in China, and take some properly measures should to curve the trend growth of obesity.

Antecedentes: En los últimos años, la obesidad es un importante problema de salud pública en muchos países. Se calcula que el 8% de los niños son obesos.Sin embargo, poco se sabe acerca de la prevalencia de la obesidad entre los alumnos de secundaria en China, el objetivo de este estudio fue evaluar la prevalencia de la obesidad en general chino, estudiante de la escuela secundaria. Métodos: Publicaciones de 2009 a 2014 en la prevalencia de obesidad entre los estudiantes de la escuela secundaria en China fueron recuperados de PubMed, Online Chinese periódico de bases de datos de texto completo de VIP, CNKI y Wan Fang.Analista del meta fue utilizado analizar el total de las tasas de obesidad para chinos de la escuela secundaria. Resultados: Después de la evaluación de la calidad de los artículos, 33 papeles fueron finalmente incluidos en nuestro estudio, y el total de los tamaños de muestra sobre la obesidad investigación fueron 218317 (107631 macho y 110686 hembra), en la que 27455 (14863 macho y 12590 mujeres) fueron la obesidad.Los resultados mostraron que el analista del meta la prevalencia de la obesidad en estudiantes de secundaria son 8,4% (IC del 95%: 6,2% - 11,3%) y 4,8% (IC del 95%: 3,2% - 7,2%) para chico y chica, respectivamente. Conclusión: Nuestros resultados sugieren que la escuela y Gobierno related Departamento debería prestar más atención a la obesidad entre los estudiantes de la escuela secundaria en China, y tomar algunas medidas adecuadamente a la curva de la tendencia de crecimiento de la obesidad.

Background: Overweight and obesity are epidemic worldwide. The purpose of this research is to estimate whether the prevalence of obesity among primary school children is high, and to identify its potential determinants to optimize the methods of prevention to combat further increases in childhood overweight. Methods: A cross-sectional study was designed to collect the routine health screening data for primary school children in Wannan area, China. Overweight and obesity status were determined using the International Obesity Task Force standard (IOTF) BMI cut-off points. Results: A total of 67956 subjects (36664 male and 31292 female) aged 5-14 years were recruited in this study. Depending on the references used (IOTF), the overall prevalence of overweight, including obesity of the subjects was 17.85% , the prevalence of overweight, including obesity was 22.9% in male subjects and 11.9% in female subjects, respectively. The overall prevalence of obesity was3.7%, the prevalence of obesity was 5.2 % in male subjects and 1.8% in female subjects, respectively. An interesting observation made was that the prevalence of overweight was high in male subjects. Conclusions: Overweight is prevalence among primary school children, especially in male children. The relate department of school and government should take some measure to reduce the prevalence of overweight and obesity.

Antecedentes: El sobrepeso y la obesidad son epidemia en todo el mundo. El propósito de esta investigacion es estimar si la prevalencia de la obesidad entre los niños de la escuela primaria es alto, y a identificar sus posibles factores determinantes para optimizar los métodos de prevención para combatir nuevas subidas en sobrepeso infantil. Métodos: Un estudio transversal fue diseñado para recoger los exámenes de salud de rutina datos para la escuela primaria los niños de 5 a 14 años en Wannan Area, China, el sobrepeso y la obesidad se determinaron utilizando la International Obesity Task Force Standard (IOTF) IMC puntos de corte. Resultados: Un total de 67956 sujetos (36664 macho y 31239 hembra) de 5 a 14 años fueron reclutados en este estudio. Dependiendo de las referencias utilizadas (IOTF), la prevalencia de sobrepeso, incluyendo la obesidad de los sujetos fue 17,85%, la prevalencia de sobrepeso, incluyendo la obesidad fue del 22,9% en sujetos masculinos y el 11,9% en mujeres, respectivamente. La prevalencia global de la obesidad was 3,7%, la prevalencia de obesidad fue de 5,2% en sujetos masculinos y 1,8% de mujeres, respectivamente. Una observación interesante es que la prevalencia de sobrepeso fue alta en sujetos masculinos. Conclusiones: El sobrepeso es la prevalencia entre los niños de la escuela primaria, especialmente en los niños varones. escuela y Departamento de Gobierno debería tomar algo de placer para reducir la prevalencia del sobrepeso y la obesidad.

OBJECTIVE: To determine whether curcumin reverses the multidrug resistance of human colon cancer cells in vitro and in vivo. METHODS: In a vincristine-resistant cell line of human colon cancer, the cell viability of curcumin-treated cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Rhodamine123 efflux was evaluated to detect P-glycoprotein transporter activity, and expression of the multidrug resistance protein 1 and survivin genes was analyzed by reverse transcription polymerase chain reaction and western blotting. In addition, xenograft mouse tumors were grown and treated with curcumin. The morphology of the xenografts was investigated by hematoxylin-eosin staining. The in vivo expression of the multidrug resistance gene and P-glycoprotein and survivin genes and proteins was observed using reverse transcription-polymerase chain reaction and western blotting, respectively. RESULTS: Curcumin was not obviously toxic to the vincristine-resistant human colon cancer cells at concentrations less than 25 μM, but the growth of cells was significantly inhibited. At concentrations greater than 25 μM, curcumin was toxic in a concentration-dependent manner. The sensitivity of cells to vincristine, cisplatin, fluorouracil, and hydroxycamptothecin was enhanced, intracellular Rhodamine123 accumulation was increased (p<0.05), and the expression of the multidrug resistance gene and P-glycoprotein were significantly suppressed (p<0.05). The combination of curcumin and vincristine significantly inhibited xenograft growth. The expression of the multidrug resistance protein 1 and survivin genes was significantly reduced in xenografts of curcumin-treated mice and mice treated with both curcumin and vincristine relative to control mice. CONCLUSION: Curcumin has strong reversal effects on the multidrug resistance of human colon carcinoma in vitro and in vivo.

Para verificar o risco potencial para hepatite viral pós-transfusional de doadores anti-HBc positivos e investigar se outros marcadores sorológicos do HBV poderiam identificar a presença ou não do vírus, mil doadores de primeira vez foram recrutados entre junho e julho de 1997. Estes doadores foram testados para os testes de rotina utilizados em centros de transfusão brasileiros. Cento e vinte desses doadores foram anti-HBc positivos (12%). Nestes foram realizados os testes HbeAg, anti-HBc, anti-HBs e a pesquisa do HBV-DNA por PCR. Dez eram HbsAg positivos, todos com presença do HBV-DNA demonstrada por PCR. Três doadores HbsAg negativos foram HBV-DNA positivos. Dois doadores HBV-DNA positivos também o foram para o anti-HBs. Todos os doadores HBV positivos apresentavam pelo menos outro marcador sorológico do HBV, além do anti-HBc. O anti-HBc é uma importante causa de descarte de sangue. A pesquisa isolada do HbsAg não é completamente protetora e a presença do anti-HBc permanece necessária como teste de triagem. A presença do anti-HBs não é sempre indicativa da ausência do vírus. A adição de outros marcadores pode ser uma alternativa para predizer a presnça do vírus quando comparada com a PCR. Outros estudos, no entanto, devem ser conduzidos para confirmar este achado.

In order to assess the potential risk of anti-HBc-positive blood donors for post-transfusional hepatitis and to investigate whether other HBV serological markers are capable of identifying the presence of the virus, 1000 first-time blood donors were enrolled between June and July 1997. These donors were screened using routine Brazilian blood center tests (HIV 1 and 2, HTLV 1 and 2, Chagas disease, Syphilis, HCV, HBsAg, anti-HBc and ALT ). The 120 (12%) found to be anti-HBc-positive underwent further tests: HBe, anti-HBe, anti-HBs and HBV-DNA by PCR. Ten cases were HBsAg positive and all were HBV-DNA positive by PCR. Three HBsAg-negative donors were HBV-DNA-positive. Two HBV-DNA-positive donors were also anti-HBs-positive. All the HBV-positive donors had at least one HBV marker other than anti-HBc. Anti-HBc is an important cause of blood rejection. Testing for HBsAg alone is not fully protective and anti-HBc remains necessary as a screening test. The presence of anti-HBs is not always indicative of absence of the virus. The addition of other HBV serological markers could represent an alternative in predicting the presence of the virus when compared with PCR. It is recommended that other studies should be carried out to confirm this finding.