RESUMO Leopardus pardalis é uma das espécies de felinos neotropicais mais estudadas em seus aspectos ecológicos, porém informações de questões morfofisiológicas do trato digestório não são encontradas na literatura. Visando contribuir com tais informações, o objetivo deste artigo foi caracterizar a morfologia do estômago dessa espécie. Nesta pesquisa, foram utilizados cinco exemplares adultos, coletados após óbito por atropelamento ou doados pelo IBAMA ao Laboratório de Zoologia e Morfologia Animal da Universidade do Estado de Mato Grosso - Alta Floresta. Os animais foram fixados com solução aquosa de formaldeído a 10%, dissecados por meio de técnicas anatômicas. Além dos estudos anatômicos, também foram coletados tecidos para microscopia. As análises macroscópicas revelaram que o estômago era do tipo unicavitário, com presença de pequena e grande curvatura, localizado no antímero esquerdo e constituído pelas regiões características do órgão. Em seus aspectos microscópicos, mostrou-se formado pelas quatro túnicas usuais do tubo digestório: túnica mucosa, submucosa, muscular e serosa, cada uma com as particularidades características de cada região, principalmente na túnica mucosa. O estômago de L. pardalis revelou uma morfologia semelhante às espécies domésticas e selvagens, com particularidades histomorfológicas na região pilórica. Esse padrão pode estar relacionado a adaptações evolutivas no processo digestivo dessa espécie.

ABSTRACT The species Leopardus pardalis is one of the species of neotropical felines more studied in its ecological aspects, however, information of morphophysiological questions of the digestive tract are not found in the literature. Aiming to contribute with such information, the objective of this work was to characterize the morphology of the stomach of this species. Five adult specimens, collected after death by trampling, or donated by IBAMA to the Laboratory of Zoology and Animal Morphology of the University of the State of Mato Grosso - Alta Floresta, were used in this research. The animals were fixed with aqueous solution of formaldehyde 10%, dissected through the basic techniques and instruments of anatomy, and later the material was collected for microscopy. The macroscopic analysis revealed that the stomach was of the unicavitary type, with small and great curvature, located in the left antimer and constituted by the characteristic regions of the organ. In its microscopic aspects it was formed by the four usual tunics of the digestive tube: tunica mucosa, submucosa, muscular and serosa, each one with the peculiarities characteristic of each region, mainly in the tunica mucosa. The stomach of L. pardalis revealed similar morphology to the domestic and wild species, with small histomorphological peculiarities in the pyloric region, which may be related to evolutionary adaptations in the digestive process of this species.

There is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vigna unguiculata) leaves infected with CPSMV both administered by gavage to Swiss mice induced a humoral immune response. Groups of 10 Swiss mice (2-month-old females) were immunized orally with 10 daily doses of either 50 µg viral capsid protein (boosters of 50 µg at days 21 and 35 after immunization) or 0.6 mg protein of the crude extract (boosters of 0.6 mg at days 21 and 35 after immunization). Anti-CPSMV antibodies were quantified by ELISA in pooled sera diluted at least 1:400 at days 7, 14, 21, 28, 35 and 42 after the 10th dose. IgG and IgA against CPSMV were produced systemically, but IgE was not detected. No synthesis of specific antibodies against the proteins of leaf extracts from V. unguiculata, infected or not with CPSMV, was detected. The use of CPSMV, a plant-infecting virus that apparently does not induce a pathogenic response in animals, induced a humoral and persistent (at least 6 months) immune response through the administration of low antigen doses by gavage. These results raise the possibility of using CPSMV either as a vector for the production of vaccines against animal pathogens or in quick and easy methods to produce specific antisera for viral diagnosis.

Foram caracterizados nove materiais genéticos de milho (Arichuna, Baraure, Braquítico, Experimental-2, Foremaíz PB, FM-6, Obregón, Proseca-71 y Tocorón) com relação a área foliar média de folhas por planta(AF), área foliar total por planta (AFT), índice de área foliar (IAF) e coeficiente de extinção de luz (K) a 0,50m, 1,00m, 1,50m, 2,00m e 2,50m de altura (a partir do solo). Foram encontrados os seguintes intervalos de variação para as diversas variáveis: área média de folhas por planta (471 cm² para Foremaíz PB e 606 cm² Baraure); área foliar total por planta: 5 327 cm² (Foremaiz PB) e 8 411 cm² (Braquítico), índice de área foliar: 4,26 (Foremaiz PB) e 6,67 (Braquítico); coeficiente de extinção de luz: 0,23 (Braquítico) e 0,42 (Arichuna); rendimento: 2 877 kg.ha-1 (Braquítico) e 4 784 kg.ha-1 (Tocorón). Não foi encontrada relação entre o rendimento e o IAF (r = 0,07), e a associação do IAF e K foi muito bem explicada pela lei de Beer.

A field experiment was carried out to evaluate nine corn genotypes (Arichuna, Baraure, B raquitico, Expe-rimental-2, Foremaiz PB, FM-6, Obregón, Proseca-71 and Tocorón) in relation to: mean leaf area per plant, total leaf area per plant (TLA), leaf area index (LAI), grain yield (Y) and light extinction coefficient (K) at 0.50m, 1.00m, 1.50m, 2.00m and 2.50m of plant height (from soil to flag leaf). Also, correlation and single regression between LAI and yield was performed. Significant genotypical differences for all variables were found, except for mean leaf area per plant Ranging was: mean leaf area per plant (471 cm² for Foremaiz PB and 606 cm² for Baraure); TLA (5,327 cm² for Foremaiz PB and 8,411 for Braquítico); LAI (4.26, Foremaiz PB and 6.67, Braquítico); K (0.23 for Braquítico and 0.42, Arichuna); Y (2, 877, Braquítico and 4,784 kg.ha-1 for Tocoron).The relationship between Y and LAI was not significant (r = 0.07). The relationship of LAI and K was described very well by Beer's law.

Se caracterizaron nueve materiales genéticos de maíz (Arichuna, Baraure, Braquítico, Experimental-2, Foremaíz PB, FM-6, Obregón, Proscca-71 y Tocorón), en relación con área promedio de hoja por planta, área foliar total por planta (AFT), índice de área foliar (IAF), y coeficiente de extinción de luz (K) a 0,50 m, 1,00 m, 1,50 m, 2,00 m y 2,50 m de altura de la planta (medidos a partir del suelo). Además, estudios de correlación y regresión simple fueron hechos entre rendimiento (R) y IAF. Se encontraron rangos de variación para las diversas variables: Area promedio de hoja por planta(471cm² para Foremaiz PB y 606 cm² para Baraure); AFT ( 5 327cm² en Foremaiz PB y 8 411 cm² para Braquítico); IAF (4,26, Foremaiz PB y 6,67, Braquítico); K (0,23 para Braquítico y 0,42, Arichuna); rendimiento (2 877 kg.ha-1, Braquítico y 4 784 kg.ha-1 en Tocorón). La relación entre rendimiento y IAF no fue significativa(r = 0,07). La asociación de IAF y K file muy bien descrita por la ley de Beer.