Abstract The objective of this study is to describe the agronomic attributes of the SCS443 Isadora apple tree cultivar and to highlight its potential use in Brazil. Obtained by the hybridization made at Epagri in 2001 between ‘Imperatriz’ and ‘Cripps Pink’, after 20 years of agronomic evaluations carried out in Caçador-SC and Fraiburgo-SC, ‘SCS443 Isadora’ was originated. This cultivar has a wide plasticity of adaptation to the southern Brazilian climate due to the medium chilling requirement to overcome dormancy. It has greater resistance to the main apple diseases than cv. Gala and fruit harvest is later than cv. Fuji. Its apples have a red skin and firm flesh (=18 lb), with high sugar content (= 14.5 %) and very crunchy and juicy texture, as well as high storage ability in post-harvesting. ‘SCS443 Isadora’ apples can be stored for 8 months, even in refrigerated air atmosphere chambers (21% O2) with inhibition of the ethylene response by the use of 1-methylcyclopropene (1-MCP). Even stored for long periods of time, the indices of quality loss, physiological disorders and fruit rotting in ‘SCS443 Isadora’ apples are lower than on Gala and Fuji apples.

Resumo O trabalho tem como objetivo descrever os atributos agronômicos da cultivar de macieira SCS443 Isadora e evidenciar suas potencialidades de uso no Brasil. Obtida pela hibridação realizada na Epagri, em 2001, entre ‘Imperatriz’ e ‘Cripps Pink’, após 20 anos de avaliações agronômicas realizadas em Caçador-SC e Fraiburgo-SC, originou-se a ‘SCS443 Isadora’. Esta cultivar é de ampla plasticidade de adaptação ao clima sul-brasileiro, em função do médio requerimento de frio hibernal, para superação da dormência das gemas. Possui maior resistência às principais doenças da macieira que a cv. Gala, e a maturação dos frutos é mais tardia que da cv. Fuji. As suas maçãs são de epiderme vermelha e de polpa firme (=18 lb.),com alto teor de açúcares (= 14,5 %) e de textura muito crocante e suculenta, bem como de muito alta capacidade de conservação em câmara fria. Maçãs ‘SCS443 Isadora’podem ser armazenadas por 8 meses, mesmo em câmaras de atmosfera do ar refrigerado (21% de O2), com a inibição da resposta ao etileno pelo uso do 1-methylcyclopropene(1-MCP). Mesmo armazenadas por longos períodos de tempo, os índices de perda de qualidade, dedistúrbios fisiológicos e de podridões são inferiores aos das maçãs Gala e Fuji.

Abstract According to literature, the apple cultivar SCS425 Luiza is a hybrid of Imperatriz♀ and Cripps Pink♂; however, molecular analysis of gametophytic self-incompatibility alleles showed the presence of an unexpected allele in SCS425 Luiza. This raised doubts about the fidelity of the pedigree. Thus, this study aimed to investigate the real genealogy of the SCS425 Luiza cultivar via fingerprint analysis. A total of 19 pairs of SSR primers covering 12 of the 17 chromosomes were used. ‘Imperatriz’ was tested as the female parent, and either ‘Cripps Pink’ or ‘Baronesa’ was tested as the presumed male parents. The results excluded ‘Cripps Pink’ as the male parent because most of the markers showed alleles of exclusion with Likelihood ratio (LR) value equal to zero. In contrast, when Baronesa was tested as the male parent, all alleles followed the expected segregation with very high LR values (>10.000). Therefore, it is concluded that the correct genealogy of the SCS425 Luiza cultivar is Imperatriz♀ and Baronesa♂.

Abstract The Apple Germplasm Bank (AGB) of Santa Catarina Agricultural Research and Rural Extension Company - Epagri, AGB-Epagri, is the largest of the genus Malus in Brazil. Twenty-eight main accessions of this bank were virus screened through DAS-ELISA, RT-PCR and IC-RT-PCR during two consecutive reproductive cycles, and each accession showed latent mixed infection by at least two species, among ASGV, ASPV and ACLSV. The combined use of diagnostic methods helped overcome inconsistencies commonly found in apple virus detection and was shown essential for the AGB-Epagri can be safely used as a source of genetic variability and for the exchange of virus-free propagative material.