Resumen Objetivo. Analizar el impacto de la pandemia por COVID-19 en las etiquetas relacionadas con el estilo de vida (alimentación, actividad física y comportamiento del sueño) entre enfermeras de la India. Método. Se realizó un estudio de corte transversal en el que participaron 942 enfermeros de todo el país. Se utilizó un cuestionario electrónico validado para evaluar los cambios en los estilos de vida antes y durante la pandemia por COVID-19. Resultados. La edad media fue de 29.01±5.7años, 53% fueron hombres. En comparación con antes de la pandemia por COVID-19, durante la misma se observó un ligero descenso en el patrón de consumo de comidas saludables (p<0.0001) y se observó una restricción de alimentos poco saludables (p<0.0001, además de una reducción de la actividad física junto con una menor participación en actividades relacionadas con el ocio (p<0.0001). El estrés y la ansiedad aumentaron ligeramente (p<0.0001). Además, el apoyo social prestado por la familia y los amigos para mantener comportamientos saludables relacionados con el estilo de vida también disminuyó significativamente durante la pandemia en comparación a antes (p<0.0001). Aunque la pandemia por COVID-19 redujo ligeramente la ingesta de comidas saludables y disuadió a los participantes de consumir alimentos poco saludables, esto puede haber conducido a una pérdida de peso individual. Conclusión. En general, se observó un impacto negativo en el estilo de vida, como la dieta, el sueño y la salud mental. Una comprensión detallada de estos factores puede ayudar a desarrollar intervenciones para mitigar la etiqueta nociva relacionada con el estilo de vida que se ha manifestado durante la pandemia COVID-19 en los enfermeros en India.

Abstract Objective. To analyze the impact of COVID-19 pandemics on lifestyle-related etiquettes like eating, physical activity, and sleep behavior among nursing staff in India. Methods. A cross-sectional descriptive E-survey was conducted among 942 nursing staff. The validated electronic survey questionnaire was used to assess the changes in lifestyle-related etiquette before and during COVID-19 Pandemic. Results. A total of 942 responses (mean age 29.01±5.7years) were collected, 53% of the respondents were men. A slight decline in healthy meal consumption pattern (p<0.0001) and a restriction of unhealthy food items were observed (p<0.0001), and also reduction in physical activity coupled with decreased participation in leisure-related activities was seen (p<0.0001). The stress and anxiety slightly increase during COVID-19 pandemics (p<0.0001). Additionally, social support extended by family and friends to maintain healthy lifestyle-related behaviors also significantly decreased during COVID-19 PANDEMIC pandemics compared to before (p<0.0001). Although the COVID-19 Pandemic slightly reduced the intake of healthy meals and deterred participants from consuming unhealthy food, this this may have led to individual weight loss. Conclusion. In general, there was a negative impact on, lifestyle like diet, sleep and mental health was observed. A detailed understanding of these factors can help to develop interventions to mitigate the harmful lifestyle-related etiquette that has manifested during COVID-19 Pandemic.

Resumen Objetivo. Analisar o impacto da pandemia de COVID-19 nos rótulos do estilo de vida (dieta, atividade física e comportamento do sono) entre enfermeiras na Índia. Método. Foi realizado um estudo transversal do qual participaram 942 enfermeiros de todo o país. Um questionário eletrônico validado foi usado para avaliar as mudanças no estilo de vida antes e durante a pandemia de COVID-19. Resultados. A média de idade foi de 29.01±5.7 anos, 53% eram homens. Comparado com antes da pandemia de COVID-19, observou-se uma leve diminuição no padrão de consumo de refeições saudáveis durante a pandemia (p<0.0001), além de redução da atividade física e menor participação em atividades relacionadas ao lazer (p<0.0001). O estresse e a ansiedade aumentaram ligeiramente (p<0.0001). Além disso, o apoio social fornecido pela família e amigos para manter comportamentos de estilo de vida saudável também diminuiu significativamente durante a pandemia em comparação com antes dela (p<0.0001). Conclusão. Em geral, foi observado um impacto negativo no estilo de vida, como dieta, sono e saúde mental. Uma compreensão detalhada desses fatores pode ajudar a desenvolver intervenções para mitigar a etiqueta prejudicial relacionada ao estilo de vida que se manifestou durante a pandemia de COVID-19 entre enfermeiras na Índia.

BaTiO3/Ba50Sr50TiO3 and BaTiO3/Ba50Sr50TiO3/SrTiO3 superlattices are characterized via Raman spectroscopy. Special attention is paid to a comprehensive analysis of their polarized Raman spectra, especially, within a soft mode (E(1TO)) range. The shift of E(1TO) soft mode is found to be more pronounced for BaTiO3/Ba50Sr50TiO3/SrTiO3 sample than for BaTiO3/Ba50Sr50TiO3, presumably owing to stronger 2D compression of BT layers and abruptly increased temperature of transition from ferroelectric to paraelectric phase.

Abstract: Background: Varicose veins and the complications of venous disease are common disorders in humans. Objective: To study the effects of bleomycin as a potential new sclerosing agent and its adverse events in treating varicose veins. Methods: Bleomycin-loaded liposomes 0.1ml was injected in the dorsal ear veins of white New Zealand rabbits. Sodium tetradecyl sulfate was used as a positive control. Normal saline was used as negative control. The blood vessels of the treated ears were photographed before and at one hour and two, eight and 45 days after treatment. Biopsies from the treated areas were obtained for histological examination. Blood samples were collected to determine any possible toxicity. Results: Bleomycin by itself was ineffective; therefore, liposomes were used as a vector to deliver bleomycin to the vein lumen. Subsequently, bleomycin started showing its sclerosing effects. Toxicity monitoring showed no apparent hematologic, pulmonary, hepatic or renal toxicities. This study revealed that bleomycin induced vasculitis, which led to vascular occlusion, which was observed on day 1 and day 8. No bleomycin-related injury was noted by histopathological examination of lung sections. The calculation of the lung/body weight coefficient indicated that edema was present in the experimental groups compared with the negative and positive controls. Study limitations: Relatively small number of experimental animals used. Conclusions: This study showed that bleomycin-loaded liposomes were able to induce vasculitis and vascular occlusion without any toxicity or complications. It might be useful, hence, to treat patients suffering from Varicose veins and other ectatic vascular diseases with this agent.

Background: Glycine oxidase (GO), a type of D-amino acid oxidase, is of biotechnological interest for its potential in several fields. In our previous study, we have characterized a new glycine oxidase (BceGO) from Bacillus cereus HYC-7. Here, a variant of N336K with increased the affinity against all the tested substrate was obtained by screening a random mutant library of BceGO. It is observed that the residue N336 is invariable between its homogeneous enzymes. This work was aimed to explore the role of the residue N336 in glycine oxidase by site-directed mutagenesis, kinetic assay, structure modeling and substrate docking. Results: The results showed that the affinity of N336H, N336K and N336R increased gradually toward all the substrates, with increase in positive charge on side chain, while N336A and N336G have not shown a little significant effect on substrate affinity. The structure modeling studies indicated that the residue Asn336 is located in a random coil between |J-18 and a-10. Also, far-UV CD spectra-analysis showed that the mutations at Asn336 do not affect the secondary structure of enzyme. Conclusion: Asn336 site was located in a conserved GHYRNG loop which adjoining to substrate and the isoalloxazine ring of FAD, and involved in the substrate affinity of glycine oxidase. This might provide new insight into the structure-function relationship of GO, and valuable clue to redesign its substrate specificity for some biotechnological application.

The mechanism of the Diels-Alder reaction of 1-(2-furyl)-3-(dimethylamino)-2-propene-1-thione with various dienophiles resulting in the formation of 2H-thiopyran derivatives were discussed by evaluating global and local electrophilicity and nucleophilicity descriptors for whole series of diene and dienophiles at B3LYP/6-31G* level of theory. The results preluded that the polarity and charge transfer flow between diene and dienophiles was consistent with the global reactivity descriptors and substitutional pattern. The local descriptors based on Parr functions proposed by Domingo were found to be quite promising to explain the regioselectivity of cycloaddition processes. For symmetrical dienophiles viz., 2, 5, 8, 11a, 11b, 13 and 14 the local descriptors concentrates equally (50%) at both interacting sites, to allocate non-regioselective cycloadditions. However, unsymmetrical dienophiles 16,18a-e and 21 have shown a preference towards a particular regioisomer and shown high regioselectivity during cycloaddition reaction. Regional nucleophilicity at the interacting site of diene were evaluated using local nucleophilicity descriptor Nk. These outcomes were found to be in exact correlation with the experimental outcomes achieved by Bogdanowicz et al.

The objective of the current study was to develop a direct, sensitive spectrophotometric method based on the oxidation of Ofloxacin using potassium permanganate in alkaline medium. The rate of change of absorbance was measured at 603 nm. The initial rate method and fixed time method (at 4 min) are utilised to construct calibration graphs for calculating the concentration of the drug. The results were validated through inter day and intraday precision assays according to the ICH guidelines and also through recovery studies. Statistical comparison of the proposed methods with that of reference method shows excellent agreement and indicates no significant difference in their accuracy and precision.

Se desarrolla un método espectrofotométrico directo y sensible basado en la oxidación de ofloxacina con permanganato de potasio en medio alcalino. La velocidad de cambio de la absorbancia fue de 603 nm. Fueron utilizados los métodos de velocidad inicial y el método de tiempo fijo (4 min) para construir las gráficas de calibración para calcular la concentración del fármaco. Los resultados fueron validados inter e intra- días por ensayos de precisión según las pautas ICH y por estudios de recuperación. La comparación estadística de los métodos propuestos con el método de referencia muestra una excelente concordancia y no existe diferencia significativa de su exactitud y precisión.