ABSTRACT The current study was conducted to investigate the influence of provitamin A biofortified maize (PABM) diet, sex and age of birds on the haematological and serum biochemistry parameters of indigenous chickens. A total of ninety-six 13 week old male and female Ovambo chickens were reared and fed on either white maize (WM) a low vitamin A diet or a PABM based diet for eight weeks. Each diet was replicated four times. Packed cell volume (PCV), haemoglobin (Hb), erythrocyte concentration (RBC), leucocytes concentration (WBC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were measured. Albumin (ALB), globulin (GLOB), total proteins (TP), triglycerides (TRI), cholesterol (CHOLES), uric acid (UA) and creatinine (CREAT) concentrations were assessed. The activity of alanine transaminase (ALT) alkaline phosphate (ALP) and aspartate transaminanse (AST) were also assayed. The PABM diet increased (p<0.05) PCV and WBC of female birds. All mean values for the haematological parameters regardless of the age, sex and diets were within the normal range. Age had an effect (p<0.05) on ALT, TP, GLOB, TRI and UA. Sex of the birds significantly influenced the TP, GLOB, ALB, CREAT and TRI levels. There was significant interaction of diet and age of bird on ALP, ALT, and GLOB concentrations. The cholesterol level in 18 weeks old male birds fed on the WM diet was above the normal range. In conclusion, feeding PABM diet to female and male indigenous chickens will not negatively impact on the health status of indigenous chickens.

BACKGROUND: Establishing profiles of physical activity (PA) is critical in tackling the chronic diseases associated with lack of PA and avoiding healthcare costs. OBJECTIVE: To investigate PA levels in urban-based South African (SA) primary school learners. METHODS: The Physical Activity Questionnaire for Older Children was completed by 7 348 learners (3 867 males and 3 481 females) aged 8 -14 years, of whom 49% were white, 39% black and 12% from other ethnic groups. Differences in PA levels by ethnic origin and province were determined using an analysis of covariance after adjusting for gender (p<0.05). Bonferroni corrections controlled for multiple comparisons. A fitted regression model examined age-related differences in PA adjusting for province. RESULTS: Of SA learners aged 8 - 14 years, 57% (n=4 224) engaged in moderate levels of PA. Thirty-one percent (n=2 247) did not meet internationally recommended amounts of moderate to vigorous physical activity. Overall, males reported higher PA levels than females (p<0.0001). PA levels declined with age from 11 to 14 years by 14% and 20% in males and females, respectively. Black learners had higher PA levels than white learners (p=0.0039). There were also significant differences in PA levels between the provinces (p<0.0001). CONCLUSION: This study provides evidence of differences in PA levels between gender, age and ethnic groups, and between provinces. A targeted approach to increase PA in high-risk populations in SA is warranted. Increased PA will help reduce the risk of chronic diseases and will contribute to the health of SA's population and the growth of the country's economy.

South African communal farmers possess indigenous breeds and resources required for organic beefproduction. However, the market off-take rate from communal farms is 5-10%, compared to 25% on commercial farms, despite the fact that 40% of cattle are found on communal lands. The objective of the study was to investigate the possibility of organic beef production on South African communal farms, using Focus Group Discussions (FGDs) conducted with communal cattle farmers in UMgungundlovu, KwaZulu-Natal. The findings revealed that socio-cultural factors are the primary reasons for keeping cattle, with cash generation being a secondary importance. Despite the lack of external support, communal farmers value their communal production systems and produce which are labelled 'organic' by default. These farmers perceive this system as wholesome; results in mature and tasty meat with several domestic and medicinal benefits compared to conventional cattle production systems, hence, its products should be sold at a premium. Socio-behavioural factors, such as isolation, inferiority and lack of institutional support, market access and market information pertaining to the marketing of organic beef are the reasons why communal farmers are not actively participating in the South African formal beef market. It can be concluded that facilitating product differentiation and the involvement of communal farmers as stakeholders in the South African formal beef market through civic engagement has the potential to increase off-take rates from communal farms, rather than the bottom-up and top-down management strategies.

ABSTRACT The study was conducted to determine the interaction of types of maize (Provitamin A-biofortified maize (PABM) versus white maize) and strain of laying birds (Amberlink and Hyline) on nutritional quality of eggs. Twenty-one of each of Amberlink and Hyline laying hens were fed on three diets for 30 days. Birds were distributed in a 3 × 2 factorial arrangement constituting three diets (0, 50 or 100 % of PABM) and two strains (Amberlink & Hyline). There was a diet × strain interaction on egg shell weight, average daily feed intake, egg production, egg weight and egg shell thickness. Eggs produced by layers under 100 % PABM had lighter eggshell weight and lower eggshell percentage. In contrast, eggs produced by layers under 50 % PABM diet had signi-ficantly higher eggshell percentage, heavier egg shell weight and thicker eggshell. As the level of PABM increased, the yellow and red hue (Hunter a* and b* values) significantly increased while the lightness values (Hunter L* values) decreased. As the level of PABM in the ration increased, the vitamin A content of the egg yolk also significantly increased. Assimilation of vitamin A from feed to egg yolk in Amberlink and Hyline hens was similar. Hence, egg enrichment with vitamin A can be achieved by using PABM in layers ration. The use of high level of PABM had a negative effect on the eggshell quality traits.