Estudo do proteinograma do líquido cefalorraqueano lombar de 40 recém-nascidos normais com 2 dias de vida. Em relação ao proteinograma do líquido cefalorraqueano do adulto normal, os achados desta investigação demonstram maior concentração proteica e maior participação relativa no perfil proteico da albumina, da globulina alfa-1 e da globulina gama. Estes achados são próprios ao perfil de tipo transudativo, encontrado em certas condições patológicas no adulto. A incompleta maturidade funcional da barreira hêmato-liquórica ao nascimento parece ser a principal responsável pelas características do proteinograma do líquido cefalorraqueano encontradas no recém-nascido normal.

The protein fractions of lumbar cerebrospinal fluid (CSF) were studied in 40 normal newborns 2 days-old. The results were compared to those found in normal adult peoples. It was shown that the relative concentrations of albumin, alpha-1 globulin, and gamma globulin are more conspicuous in the newborn than in the adult. The protein pattern in the normal newborn is similar to the transudative pattern described in the adult life in several pathologic conditions. The findings were related to the blood — CSF barrier conditions in the newborn. The incomplete functional maturity of blood — CSF barrier at this time of the life was considered to be the main factor responsible by the CSF protein pattern which was found.

Foi estudado o perfil citológico do LCR de 21 recém-nascidos normais com 2 ou 7 dias de idade, os preparados tendo sido obtidos mediante sedimentação gravitacional acelerada. Para citometria normal (até 12 leuecocitos e até 600 hemácias por mm³), o perfil citológico era constituído de: linfocitos (11 a 59%), reticulomonocitos (41 a 78%), polinucleares neutrófilos (0 a 17%), células eosinófilas (0 a 2%); macrófagos (0 a 10%). Em média, as seguintes percentagens representam o perfil: linfocitos 34,6; reticulomonocitos 60,4; polinucleares neutrófilos 2,9; células eosinófilas 0,2; macrófagos 1,9. Podem ser observados também agrupamentos celulares: de células ependimárias possivelmente do revestimento corióideo; de células reticulares indiferenciadas, possivelmente da aracnóide.

Total cell count of cerebrospinal fluid (CSF) in normal newborn infants has shown no more than 12 leucocytes and 600 red cells by cubic millimeter, as it was previously reported by one of us5. Results on CSF cytomorphology are reported in this study. CSF cytomorphology was studied in 21 normal newborn infants: 2 days old (17 cases) and 7 days old (4 cases). The samples were collected through lumbar puncture. Cytomorphological examinations were carried out after preparing the samples by accelerated gravitational sedimentation in Suta's chamber; the sediments were stained by Leishman's technique. Cytomorphology was type II in most of the cases. Lymphocytes and reticulomonocytes were found in all cases, the relative proportion being 11-59% and 41-78% respectively. Polinuclear neutrophils were found in 12 cases, and the higher relative proportion observed for them was 17%. Eosinophil cells occurred in 3 cases, in the proportion of no more than 2%. Macrophages occured in 12 cases, and the higher proportion observed was 10%. The average values were: lymphocytes 34,6%, reticulomonocytes 60.4%, polinuclear neutrophils 2.9%, eosinophil cells 0.2%, macrophages 1.9%. Linear cell groupings occurred in 3 cases; their nature was morphologically related to choroid cells. Polymorph groupings of undiferenciated reticular cells were found also. They occurred in 5 cases, and their origin was related to cells of arachnoid membrane.