In December 2019, the first case of severe acute respiratory syndrome (COVID-19), caused by the new coronavirus (SARS-CoV-2) was reported and the disease was declared as a worldwide pandemic in March 2020. As there is no vaccine currently available, the daily hygiene cleaning habits and social distance are the ways to fight COVID-19 indicated by the WHO. Hand and surface hygiene has become essential and the use of 60-90% ethanol is recommended for its effective biocide action. Phenol-based products are effective sanitizers, but they can cause irritation and are not recommended for surfaces that come into contact with food. There are quaternary ammonium salts that present low toxic and are good sanitizers, as they can cause the denaturation of proteins and disruption of viral lipid bilayer. Sodium hypochlorite is one of the most used sanitizers due to its low cost and effective oxidative capacity. In the same direction, peroxides are also used as a biocide, as they cause oxidation of lipids and denaturation of viral proteins. The goal of this paper is to describe the action modes and chemical concepts of the sanitizers recommended by the ANVISA-Brazil to be used against the new coronavirus.

The simultaneous screening of multiple enzyme activities in a single assay has numerous advantages over the traditional format, since it decreases sampling errors, allows savings in reagents and consumables and reduces the time and labor required to conduct the assays. In the present study, a direct and sensitive assay for the simultaneous detection of epoxide hydrolase and esterase (or lipase) activities was developed. Signal overlap is avoided by synthesizing fluorogenic probes with enzyme-specific alkyl linkers, connected to different fluorophores (resorfurin and umbelliferone), which exhibit emission spectra at different wavelengths. The simultaneous assays were conducted in microplate format with the fluorogenic probes monitored in the same well that uses microorganisms as enzyme source. Our results show that the fluorescent signal from each of the probes used here can be discriminated, allowing multiple enzyme activity detection and quantitation.

High throughput techniques to evaluate enantiomeric excess (ee) and enantiomeric ratio (E) of enzymatic reactions are fast and efficient tools that can be applied large quantities of enzymes, microorganisms and clones. The Quick-ee technique proposed in this study produces the ee and conversion values separately.

Descrevemos neste trabalho uma síntese curta e eficiente para o fragmento C1-C9 do potente agente antitumoral dictiostatina. Esta abordagem sintética inclui uma reação de epoxidação assimétrica de Sharpless seguida da abertura de epóxido nas condições de Myashita para introduzir os centros estereogênicos em C6 e C7 e uma reação de olefinação tipo Horner-Wadsworth- Emmons nas condições de Ando para construir a ligação com geometria /Z/do dieno 1,3-/Z/,/E/.

We describe herein a short and efficient synthesis of the C1-C9 fragment of the potent antitumor agent dictyostatin. Notable features of this approach include a Sharpless asymmetric epoxidation followed by epoxide opening under Myashita's conditions to introduce the stereogenic centers at C6 and C7, and a Horner-Wadsworth-Emmons type reaction under Ando's conditions to construct the Z-double bond of the 1,3-(Z,E)-diene system.

Descrevemos neste trabalho nossa rota sintética para obtenção do policetídeo antifúngico (-)-basiliskamida A, assim como estudos de RMN de ¹H relacionados a migração da cadeia lateral cinamoíla da basiliskamida A para formar a basiliskamida B em CDCl3.

We describe herein our approach to the total synthesis of the antifungal polyketide (-)-basiliskamide A, as well as ¹H NMR studies on the migration of the cinnamoyl side chain of basiliskamide A to form basiliskamide B in CDCl3 solution.