Abstract Objective The present study aims to highlight the significance of the nucleic acid test (NAT) for musculoskeletal tissue donation and to compare the sensitivity of this test on the different available platforms. Method The present study is a retrospective survey in a human tissue bank database and an integrative literature review encompassing the last 10 years. The PubMed portal and the SCOPUS, CINAHL, and Web of Science databases were queried for articles. Results We found no specific studies on the use and sensitivity of NAT in braindead tissue donors. The information presented in the present study consists of specific contents intended for the Brazilian Blood Transfusion Network (Hemorrede Transfusional Nacional, in Portuguese) and internal retrospective data from a tissue bank located at a city in the state of São Paulo, Brazil. Conclusions The NAT is effective in blood samples from living patients. However, since biochemical reactions in braindead patients can be different, specific research, platforms, or both are crucial to tissue banks.

Resumo Objetivo Evidenciar a importância da realização do teste de ácido nucleico (NAT, na sigla em inglês) para doação de tecidos musculoesqueléticos, assim como comparar a sensibilidade deste exame nas diferentes plataformas existentes no mercado. Método Trata-se de um levantamento retrospectivo no banco de dados de um determinado Banco de Tecidos Humanos e de uma revisão integrativa da literatura, operacionalizada nos últimos 10 anos. As buscas de artigos ocorreram no portal PubMed e nas bases de dados SCOPUS, CINAHL e Web of Science. Resultados Não foram encontrados estudos específicos sobre a utilização e a sensibilidade do exame NAT em pacientes doadores de tecidos com morte encefálica (ME), sendo as informações apresentadas no presente estudo conteúdos específicos destinados à Hemorrede Transfusional Nacional e aos dados retrospectivos internos de um Banco de Tecidos do interior do estado de São Paulo, Brasil. Conclusões O exame NAT se apresenta efetivo em amostras de sangue de pacientes vivos. Porém, reações bioquímicas em pacientes com condições de ME podem se apresentar de formas diferenciadas, tornando-se indispensáveis a realização de pesquisas específicas e/ou a indicação de plataformas aos Bancos de Tecidos.

Abstract The objective of this study was to assess the efficiency of essential oils of cloves (Syzygium aromaticum) and thyme (Thymus vulgaris) on the control of Acanthoscelides obtectus in laboratory conditions. The extraction of the oils was executed by the hydro-distillation method in a Clevenger device, for 4 hours and the design used was completely randomized, with five replications, in a 10x8 factorial arrangement (dozes and exposition time) with ten concentrations (20; 10; 5; 2.5; 1.75; 0.75; 0.5; 0.25 and 0.0% and Tween® 5.0%). Each replication was comprised by 10 unsexed insects of A. obtectus. The variables evaluated are as follows: control efficiency and CL50 through the Proc Probit analysis. The evaluations were carried out in 1, 2, 3, 12, 24, 48, 72, and 96 hours after the treatment. The results demonstrate that the essential oils of cloves and thyme caused 100% of mortality after 48 and 72 hours, respectively, in the 20% concentration. The Cl50 was estimated at 30.46 µL mL-1 for the oil of cloves and 24.93 µL mL-1 for the oil of thyme. The use of essential oils of cloves and thyme represent a viable alternative for use in storehouses for the integrated management of A. obtectus.

Abstract The study was carried out on an agroecological property located in Cologne São Manoel, 8th district of Pelotas, RS, Brazil. Anochetus neglectus Emery, 1894 was collected in an area of peach orchard that has been under an agroecological system for 18 years. This record expands the area of occurrence and distribution of this rare species in the Neotropical Region.

Abstract Ceratitis capitata (Wiedemann, 1824) is an insect of major economic importance in the mango orchards of the submedium of the São Francisco River Valley, the main area of mango production and exportation in Brazil. To provide alternatives for the management of C. capitata, toxic baits based on alpha-cypermethrin (Gelsura®) and spinosad (Success® 0.02 CB) were evaluated in three commercial mango experiments during two consecutive harvests: 2016/2017 (experiment 1 - area 1) and 2017/2018 (experiment 2 -area 2 and experiment 3 - area 3). According to the results, there was a large reduction in the infestation of C. capitata after five sequential applications of the alpha-cypermethrin (6 g.ha-1) and spinosad (0.38 g.ha-1) toxic baits performed at seven-day intervals during mango fruit ripening in all experiments and years (harvest) evaluated. Compared with the untreated plots, the plots with alpha-cypermethrin and spinosad applications showed a significant reduction in the damage induced (fallen fruits and/or on trees) by C. capitata. The management of C. capitata in mango orchards can include the use of the toxic bait based on alpha-cypermethrin, which represents an alternative to rotate with spinosad toxic bait in the São Francisco River Valley.

RESUMO Objetivo: Avaliar a influência da contratura em flexão da articulação do quadril sobre a lordose lombar, parâmetros espinopélvicos e as alterações desses parâmetros após a artroplastia total do quadril (ATQ). Métodos: Vinte pacientes adultos com artrose do quadril e indicação de ATQ foram divididos em dois grupos, sendo 10 pacientes com contratura em flexão da articulação do quadril e 10 sem contratura. Os pacientes foram avaliados no pré-operatório e seis meses após a realização da ATQ, através dos seguintes parâmetros radiográficos: eixo vertical sagital (SVA), lordose lombar (LL), incidência pélvica (PI), inclinação do sacro (SS) e versão da pelve (PT). Resultados: Não foi observado diferença estatística entre os valores da LL pré e pós-operatória nos grupos avaliados. Após a realização da ATQ, houve um aumento da PT nos dois grupos, e redução da SS somente no grupo de pacientes sem contratura em flexão do quadril. Conclusão: Os pacientes com artrose do quadril e contratura em flexão da articulação do quadril apresentam tendência para uma LL aumentada na posição ortostática comparado aos pacientes sem contratura, porém sem significância estatística. Após a realização da ATQ foi observado aumento da PT em ambos os grupos e redução da SS nos pacientes sem contratura em flexão da articulação do quadril. O papel da contratura em flexão do quadril na mobilidade da pelve e nos parâmetros espinopélvicos deve ser estudado com maior profundidade para melhor compreensão destas relações. Nível de Evidência III, Estudo de Caso-Controle.

ABSTRACT Objective: To assess the influence of hip flexion contracture on lumbar lordosis and spinopelvic parameters and the changes in these parameters after total hip arthroplasty (THA). Methods: Twenty adult patients with hip osteoarthritis were divided into two groups (ten patients with hip flexion contracture and ten without contracture). Patients were assessed preoperatively and six months after THA using the radiographic parameters sagittal vertical axis (SVA), lumbar lordosis (LL), pelvic incidence (PI), sacral slope (SS), and pelvic tilt (PT). Results: No statistical difference was found between pre- and postoperative LL values in the groups. After THA, both groups had increased PT and the group without hip flexion contracture had reduced SS. Conclusion: Patients with hip osteoarthritis and hip flexion contracture tend to have an increased LL in the orthostatic position compared to patients without contracture, but with no statistical significance. After THA, PT increased in both groups and SS decreased in patients without hip contracture. Studies should further investigate the role of hip flexion contracture on pelvic mobility and spinopelvic parameters to better understand these relations. Level of Evidence III, Case-Control Study.

Abstract The objective of the study was to compare the richness and diversity of ant assemblages in an agroecological system under peach orchard, conventional system under peach orchard cultivation and native vegetation in rural properties located in a Pampa Biome. The study was conducted in four samplings in 2017: 1st and 09th March (summer); 24th and 31st July (winter); and four samplings in 2018: 23rd and 30th January (summer); 31st July and 07th August (winter). Pitfall traps were used. The assemblages were characterized and compared using richness, number of occurrences of ants, Shannon diversity (H’), equitability, rarefaction analysis and Chao 1. The association of the species with the samples was evaluated by a Principal Component Analysis (PCA). The agroecological system had the highest number of occurrences, while the conventional orchard the lowest number. Richness and abundance were greatest during the summer. The conventional peach orchard obtained the lowest H’ for both seasons when compared to the agroecological orchard and native vegetation. The PCA explained 77.40% of the occurrence of ants in the environments and in the seasons. The results found demonstrated that conservationist systems tend to harbor greater wealth and diversity of ant assemblages, as well as occurring in native áreas.

Abstract Anastrepha fraterculus (Wiedemann, 1830) (Diptera: Tephritidae) is a major fruit pest, which is basicaly controlled using insecticides, which represents a risk to beneficial arthropods, human health and food contamination. The sterile insect technique (SIT) is a potential alternative tool for the management of this pest, however, only conflicting data is found regarding the optimal dose to achieve sterility. Thus, this study evaluated the effect of gamma radiation doses (0, 40, 50, 60 and 70 Gy) on male and female reproductive sterility, gonads morphometry, emergence, flight ability, and longevity under nutritional stress of A. fraterculus. Full female sterility was achieved at 50 Gy, while full male sterility was achieved at 70 Gy. Both ovarian and testicular sizes were affected by irradiation, while no influence was observed on the quality parameters evaluated. Our results suggest that 70 Gy applied 48 h before adult emergence can be used to sterilize A. fraterculus in a SIT programme.

RESUMO: Anastrepha grandis é uma das principais pragas relacionadas à Cucurbitaceae nas Américas do Sul e Central. O presente trabalho teve como objetivo conhecer o impacto do aumento da temperatura no número de gerações de A. grandis, cuja distribuição poderá ser agravada devido ao aumento da temperatura. Essas variações climatológicas foram analisadas para cenários de referência obtidos de 1961‒1990 e nos cenários de mudanças climáticas A2 e B1 do Painel Intergovernamental de Mudanças Climáticas, onde encontramos um cenário menos pessimista (B1) e um mais pessimista (A2). Em relação ao período de referência é possível observar que, nas estações mais frias, as regiões Sul e Sudeste mostram-se inadequadas para o desenvolvimento de A. grandis, apresentando no máximo uma geração, enquanto nas estações mais quentes o inseto pode chegar a duas gerações. Nas demais regiões do país, onde as temperaturas apresentam-se mais elevadas durante todo o ano, o número de gerações é de no mínimo duas e não há variação de uma estação climática para outra. Quando analisado o aumento da temperatura, em um cenário mais pessimista (A2), é possível observar uma variação considerável no número de gerações nos três cenários climáticos futuros, podendo A. grandis dobrar o número de gerações. Em relação a um cenário menos pessimista (B1), é evidente uma variação menor no número de gerações, principalmente na região Sul do país, enquanto que na região Sudeste essa variação já é mais acentuada devido ao aumento da temperatura, podendo dobrar o número de gerações mesmo nas estações mais frias.

ABSTRACT: Anastrepha grandis is one of the main pests related to Cucurbitaceae in South and Central America. This study discusses the impact of temperature increase on the number of generations of A. grandis, whose distribution could be aggravated due to temperature increase. Climatic variations were analyzed for reference scenarios obtained from 1961‒1990 and of A2 and B1 climatic change scenarios of the Intergovernmental Panel on Climate Change, in which a less pessimistic scenario (B1) and a more pessimistic scenario (A2) were found. In relation to the reference scenarios, in colder seasons, the southern and southeastern regions are inadequate for the development of A. grandis, presenting one generation at most. In other regions of Brazil, where temperatures are higher throughout the year, the number of generations is at least two, and there is no variation from one climatic season to another. When analyzing the temperature increase, in a more pessimistic scenario (A2), there is a considerable variation in the number of generations, if we take into account three future climate scenarios in which A. grandis practically doubles the number of generations. In relation to a less pessimistic scenario (B1), there is a smaller variation in the number of generations, mainly in the southern region of the country. This variation is more accentuated in southeastern Brazil due to the temperature increase, in which the pest’s number of generations doubles even in colder seasons.

Abstract As native tree species are often used for densification, enrichment and degraded areas recovery, a better understanding of the behavior of their seedlings in response to light exposure is needed. This study evaluates the growth and morphological behavior of five native forest species of the Brazilian Mixed Ombrophilous Forest under shading levels of 90%, 50%, and 0%. The species were measured for height to root collar diameter (RCD) ratio (H:D), leaf area (LA), Dickson’s quality index (DQI), and stem, leaf, and root biomass. The LA did not differ among the treatments for three species and did not differ among species in full sun. The results for H:D ratio, DQI, and biomass indicate better growth in treatments with greater light availability. The species are recommended to be used as follows: Cedro-vermelha in full sun; Tarumã-azeitona, Ingá-doce, and Ocotea-guaicá in varying environmental conditions; and Branquilho-bravo in 50% shade and full sun conditions.

RESUMO: A drosófila-da-asa-manchada, Drosophila suzukii Matsumura, é considerada o principal inseto-praga de pequenos frutos no mundo. A identificação de hospedeiros suscetíveis é essencial para o desenvolvimento de estratégias de manejo. O objetivo deste estudo foi verificar o nível de infestação de D. suzukii em frutos de amoreira-preta, mirtileiro, morangueiro, araçazeiro e pitangueira cultivados na região no sul do Brasil e determinar os índices de infestação (II) por fruto. Os estudos foram realizados durante duas safras (2015/16 e 2016/17) em três áreas com cultivo orgânico de pequenas frutas. O maior nível de infestação natural de D. suzukii foi observado em amora-preta (40 a 65% de infestação) e morango (aproximadamente 30% de infestação). Em contraste, o mirtilo foi menos preferido (< 7% de infestação). Para as frutas nativas da região (araçá e pitanga), também ocorreu infestação da mosca, demonstrando serem hospedeiros potenciais para a multiplicação da praga nos períodos de entressafras. Em relação ao II, amoras e pitangas proporcionaram um maior número médio de insetos por grama de fruto (≈ 1,6 adultos de D. suzukii). Por outro lado, mirtilos e araçás apresentaram a menor relação de insetos emergidos por gramas de frutos (≈ 0,7 adultos por grama). O período entre final da primavera até meados do outono foi considerado ideal para a ocorrência da praga no campo. O conhecimento dos níveis de infestação natural é fundamental para o entendimento do comportamento da praga no campo, o que auxiliará no delineamento de estratégias de manejo.

ABSTRACT: The spotted-wing drosophila, Drosophila suzukii Matsumura, is considered the main insect pest of small fruit in the world. Identifying susceptible hosts is essential to develop management strategies. The aim of this study was to verify the level of D. suzukii infestation in blackberry, blueberry, strawberry, cattley guava and Surinam cherry fruits cultivated in the southern region in Brazil, and to determine the infestation index (II) per fruit. The studies were carried out during two harvests (2015/16 and 2016/17) in three areas with organic cultivation of small fruit. The highest level of D. suzukii natural infestation was observed in blackberry (40 to 65% infestation), and strawberry (approximately 30% infestation) fruits. In contrast, blueberries were less preferred (< 7% infestation). For native fruit of the region (cattley guava and Surinam cherry), it was also verified infestation of the fly, demonstrating they are hosts for multiplication in the off-season periods of the other crops. In relation to II, blackberry and Surinam cherry provided a higher average number of insects per gram of fruit (≈ 1.6 adults of D. suzukii). In contrast, blueberries and cattley guava had the lowest insect ratio per gram of fruit (≈ 0.7 adults per gram). The period between late spring to mid-autumn was considered ideal for the pest occurrence in the field. The knowledge of natural infestation levels is essential for understanding the behavior of the pest in the field, which will assist in the design of management strategies.

ABSTRACT Neotropical Annonaceae-derived bioinsecticides have shown promising control of insect pest species. In order to develop an alternative tool for control Zaprionus indianus (Gupta, 1970), an important invasive pest of Brazilian orchards, this study investigated the insecticidal activity of a formulation prepared from ethanolic seed extract of different Annona species (Annona mucosa Jacq., Annona muricata L., and Annona sylvatica A. St.-Hil.) at discriminatory concentration of 2000 mg L-1 in comparison with a limonoid-based bioinsecticide and a synthetic insecticide (spinetoram) as positive controls. In an ingestion and topical application bioassay, the formulation based on A. mucosa caused more than 95% mortality, which was equivalent to the mortality caused by the synthetic insecticide spinetoram. In a free-choice bioassay, A. mucosa (83%), bioinsecticide (88%) and synthetic insecticide (98%) produced a significant reduction in the number of eggs per fruit. In addition, formulations based on A. mucosa, A. sylvatica, A. muricata and bioinsecticide resulted in a 50% reduction in egg viability. In the field, A. mucosa and bioinsecticide negatively influenced the infestation by Z. indianus in figs, producing a significant reduction in the number of larvae per fruit (3.6 and 2.29 larvae per fruit, respectively) in relation to the control treatment (11 larvae per fruit). However, they were significantly inferior to the synthetic insecticide (0.71 larvae per fruit). Thus, A. mucosa, bioinsecticide and spinetoram have the potential to be used in the framework of Z. indianus management.

An alternating magnetic field (AMF)-stimuli responsive nanodevice based on magnetic nanoparticles (MNP) functionalized with water-soluble carboxylate-substituted pillar[5]arene (CP[5]A), namely MNP-CP[5]A, as a multiplatform for cancer treatment has been designed. MNP-CP[5]A was loaded with doxorubicin (DOX), showing a loading capacity of 9.5 mg g-1. The nanodevice demonstrated good colloidal stability, superparamagnetic behavior, and was capable to generate detectable heat in solution induced by AMF application. DOX release, monitored by ultraviolet-visible (UV-Vis) spectroscopy, was investigated by varying the temperature (37 and 45 °C) without AMF and in the presence of AMF (frequency (f) = 307 kHz, field amplitude (H) = 200 Oe, 45 °C) at pH = 7.4. Thermo-induced DOX release without AMF was 1.9% (1.8 μg mL-1) and 2.3% (3.3 μg mL-1) at 37, and 45 °C within 50 min, respectively. In an AMF DOX release increased to 5.7% (8.2 μg mL-1) within 50 min. Therefore, MNP-CP[5]A-DOX works as a chemo-hyperthermia nanodevice.

RESUMO Apoharknessia eucalyptorum foi inicialmente descrita em 2017 e identificada em folhas de Eucalyptus dunnii no Sul do Brasil. No entanto, faltam informações sobre o cultivo in-vitro para estudos complementares. No presente estudo folhas de E. dunnii foram inoculadas para demonstrar a patogenicidade de A. eucalyptorum, e o crescimento e esporulação foram avaliados em temperaturas de 15, 20 e 25°C em quatro meios de cultura: extrato de malte-ágar (MEA), batata dextrose-ágar (PDA), suco V8-ágar (V8) e feijão dextrose-ágar (BEAN), sob luz constante. Apoharknessia eucalyptorum causou manchas foliares nas folhas inoculadas. As melhores condições para o crescimento micelial foram em 25°C em PDA, BEAN e MEA. Para a esporulação as melhores condições foram a 25°C em todos os meios testados, e também a 20°C em PDA e BEAN. As características das colônias mudaram com as temperaturas, em 15°C as colônias formaram micélio cotonoso, enquanto que em 25°C o micélio cresceu espalhado pelo meio de cultura formando margens escuras limitadas por micélio branco manchado e conídios. As condições indicadas para o crescimento e esporulação in-vitro de A. eucalyptorum são os meios MEA, PDA e BEAN em 25°C.

ABSTRACT Apoharknessia eucalyptorum was first described in 2017 and identified on leaves of Eucalyptus dunnii in Southern Brazil. However, information about in vitro cultivation for complementary studies is lacking. In the present study, leaves of E. dunnii were inoculated to demonstrate the pathogenicity of A. eucalyptorum, and growth and sporulation were evaluated at temperatures of 15, 20, and 25°C on four culture media: malt extract agar (MEA); potato dextrose agar (PDA); V8 juice agar (V8); and bean dextrose agar (BEAN), under constant lighting. Apoharknessia eucalyptorum caused leaf blight on the inoculated leaves. The best conditions for mycelial growth were at 25°C on PDA, BEAN and MEA. Considering sporulation, optimal conditions were 25°C for all tested media and 20°C for PDA and BEAN. Colony characteristics changed with temperature; at 15°C colonies formed a fluffy mycelium, whereas at 25°C mycelium spread across the medium forming dark margins lined by dirty-white mycelium and conidia. The conditions indicated for in vitro growth and sporulation of A. eucalyptorum are the culture media MEA, PDA and BEAN at 25°C

RESUMO Objetivo: Determinar se o uso tópico de gentamicina reduz a taxa de infecção articular periprotética na artroplastia total primária do quadril. Métodos: Avaliamos retrospectivamente dois coortes de pacientes submetidos à artroplastia total primária do quadril em um hospital universitário, com seguimento pós-operatório mínimo de 1 ano e completa documentação clínica, laboratorial e radiológica. Os casos operados nos primeiros 59 meses do período do estudo (263 quadris) utilizaram somente a cefazolina por via endovenosa como antibioticoprofilaxia (Grupo Cef). Os casos operados nos 43 meses seguintes (170 quadris) utilizaram a cefazolina por via endovenosa associada à gentamicina tópica aspergida diretamente na ferida operatória como antibioticoprofilaxia (Grupo Cef + Gen). Para o diagnóstico de infecção articular periprotética, utilizamos os critérios do Centers for Disease Control and Prevention. Os dados foram submetidos ao teste exato de Fisher, e valor de p menor que 0,05 foi considerado significativo. Resultados: Treze quadris apresentaram infecção articular periprotética no Grupo Cef (4,9%) e oito quadris no Grupo Cef + Gen (4,7%). A análise estatística demonstrou não haver diferença entre estas taxas (p=1,0). Conclusões: O uso tópico da gentamicina, da maneira como utilizada neste estudo, não reduziu a taxa de infecção articular periprotética na artroplastia total primária do quadril. Nível de evidência III, Estudo comparativo retrospectivo.

ABSTRACT Objective: To determine whether the topical use of gentamicin reduces periprosthetic joint infection rates in primary total hip arthroplasty (THA). Methods: We retrospectively evaluated two cohorts of patients who underwent primary THA in a university hospital, with a minimum of 1-year postoperative follow-up and full clinical, laboratory, and radiological documentation. Patients who underwent operation in the first 59 months of the study period (263 hips) received only intravenous cefazolin as antibiotic prophylaxis (Cef group), and those who underwent operation in the following 43 months (170 hips) received intravenous cefazolin plus topical gentamicin directly applied on the wound as antibiotic prophylaxis (Cef + Gen group). For the diagnosis of periprosthetic joint infection, we used the criteria of the Centers for Disease Control and Prevention. Data were analyzed using the Fisher exact test, and p values of <0.05 were considered significant. Results: Thirteen hips (4.9%) in the Cef group and eight hips (4.7%) in the Cef + Gen group presented periprosthetic joint infection. Statistical analysis revealed no difference between the infection rates (p = 1.0). Conclusion: Topical gentamicin as used in this study did not reduce periprosthetic joint infection rates in primary THA. Level of Evidence III, Retrospective comparative study.

Abstract A new species of microgastrine wasp, Cotesia invirae Salgado-Neto & Whitfield, sp. nov., is described from southern Brazil in Santa Maria, Rio Grande do Sul. This species is a koinobiont gregarious larval endoparasitoid and spins a common mass of cocoons underneath the host caterpillars of Opsiphanes invirae (Huebner, 1818) (Lepidoptera: Nymphalidae), feeding on the palm trees Syagrus romanzoffianum (Cham.) Becc.; Livistona chinensis (N.J. Jacquim) R. Brown; Roystonea regia (HBK) O.F. Cook; Archontophoenix cunninghamiana Wendland & Drude (Palmaceae). Morphological, molecular, biological, ecological and geographical data are integrated to describe the new species.