ABSTRACT Objective: to identify the profile of microorganisms isolated from biological samples of patients admitted to a hospital in Paraná. Method: descriptive and retrospective research, with data collection from a secondary source, developed in a hospital in Paraná (Brazil), referring to 2018. The study population consisted of biological samples from 226 patients admitted during the period from January 1st to December 31st, 2018. Data analysis was performed using descriptive statistics. Result: the mean age of the patients was 60.41 years old and the majority were women. Of the biological samples, 70 had positive results for bacterial identification. The tracheal aspirate showed greater positivity to microorganisms, with 30%. The most frequent microorganisms were Escherichia coli, coagulase-negative Staphylococcus, Pseudomonas, Staphylococcus aureus, Enterobacter sp. and Proteus sp. Conclusion: the study contributes to hospital care, as it produces knowledge of the microbiological profile and its resistance to antibiotics.

RESUMEN: Objetivo: identificar el perfil de microorganismos aislados de muestras biológicas de pacientes internados en un hospital de Paraná. Método: investigación descriptiva e retrospectiva, con recolección de datos de una fuente secundaria, desarrollada en un hospital de Paraná (Brasil), con referencia al año 2018. La población del estudio estuvo compuesta por muestras biológicas de 226 pacientes internados durante el período del 1 de enero al 31 de diciembre de 2018. El análisis de los datos se realizó por medio de estadística descriptiva. Resultado: la media de edad de los pacientes fue de 60,41 años y la mayoría eran mujeres. De las muestras biológicas, 70 tuvieron resultados positivos con respecto a la identificación bacteriana. El aspirado traqueal presentó mayor positividad a microorganismos, con 30%. Los microorganismos más frecuentes fueron Escherichia coli, Staphylococcus coagulasa negativa, Pseudomonas, Staphylococcus aureus, Enterobacter sp. y Proteus sp. Conclusión: el estudio contribuyó a la asistencia hospitalaria, por generar conocimiento del perfil microbiológico y su resistencia a antibióticos.

RESUMO Objetivo: identificar o perfil de microrganismos isolados de amostras biológicas de pacientes internados em um Hospital do Paraná. Método: pesquisa descritiva e retrospectiva, com coleta de dados de fonte secundária, desenvolvida em um hospital do Paraná (Brasil), referente a 2018. A população do estudo foi composta por amostras biológicas de 226 pacientes admitidos durante o período de 01 de janeiro a 31 de dezembro de 2018. A análise dos dados foi realizada pela estatística descritiva. Resultado: a idade média dos pacientes foi de 60,41 anos e maioria mulheres. Das amostras biológicas, 70 tiveram resultados positivos para identificação bacteriana. O aspirado traqueal apresentou maior positividade a microrganismos, com 30%. Os microrganismos mais frequentes foram Escherichia coli, Staphylococcus coagulase negativa, Pseudomonas, Staphylococcus aureus, Enterobacter sp. e Proteus sp. Conclusão: o estudo contribui para a assistência hospitalar, por produzir conhecimento do perfil microbiológico e sua resistência a antibióticos.

RESUMO OBJETIVO: Além de suas propriedades anticoagulantes, a heparina apresenta efeitos vasodilatadores e anti-inflamatórios que podem ajudar na reversão das alterações sépticas da microcirculação. Este artigo investiga os efeitos da heparina sobre alterações da microcirculação relacionadas com endotoxemia e compara-os com os observados com o uso de proteína C humana recombinante ativada. MÉTODOS: Após os procedimentos de implantação da câmara de dobras cutâneas e indução de endotoxemia por administração intravenosa de lipopolissacarídeo da Escherichia coli (2mg.kg-1), hamsters sírios dourados machos foram tratados com heparina não fracionada intravenosa (0,2 mg. kg-1). A microscopia intravital de preparações de câmara de dobras cutâneas permitiu a análise quantitativa de variáveis microvasculares e venulares, bem como de rolamento e aderência de leucócitos. Parâmetros micro-hemodinâmicos também foram analisados. Hamsters endotoxêmicos tratados com proteína C humana recombinants ativada e animais não tratados serviram como controles. RESULTADOS: A heparina diminuiu o rolamento de leucócitos e a vasoconstricção arteriolar induzida por lipopolissacarídeo e produziu um aumento de sobrevida em comparação com animais não tratados, enquanto proteína C humana recombinants ativada diminuiu a adesão de leucócitos. A administração de heparina mais proteína C humana recombinante ativada resultou numa significativa atenuação dos déficits de perfusão capilar induzida por lipopolissacarídeo. CONCLUSÕES: A heparina produz efeitos protetores sobre a microcirculação dos animais endotoxêmicos. Estas vantagens foram potenciados quando a heparina foi administrada em combinação com proteína C humana recombinante ativada.

OBJECTIVE: Apart from its anticoagulant properties, heparin has vasodilator and anti-inflammatory effects that could assist in the reversal of septic microcirculatory changes. This paper investigates the effects of heparin on endotoxemia-related microcirculatory changes and compares them to those observed with the use of recombinant human activated protein C. METHODS: After skinfold chamber implantation procedures and endotoxemia induction by intravenous Escherichia coli lipopolysaccharide administration (2 mg.kg-1), male golden Syrian hamsters were treated with intravenous unfractionated heparin (0.2 mg.kg-1). Intravital microscopy of skinfold chamber preparations allowed quantitative analysis of microvascular variables and venular leukocyte rolling and adhesion. Macrohemodynamic parameters were also analyzed. Endotoxemic hamsters treated with recombinant human activated protein C and non-treated animals served as controls. RESULTS: Heparin decreased lipopolysaccharide-induced leukocyte rolling and arteriolar vasoconstriction; it also increased survival when compared with non-treated animals, while recombinant human activated protein C decreased leukocyte adhesion. Administration of heparin plus recombinant human activated protein C was associated with a significant attenuation of lipopolysaccharide-induced capillary perfusion deficits. CONCLUSIONS: Heparin yields protective effects on endotoxemic animals' microcirculation. Those benefits were potentiated when heparin was administered in conjunction with recombinant human activated protein C.

OBJECTIVE: This study investigated the effects of buflomedil and pentoxifylline, both of which are used in reconstructive surgery of hamster skin flap microcirculation, and evaluated the skin flap survival rate by orthogonal polarization spectral imaging. METHOD: Twenty-four adult male Syrian golden hamsters were divided into three groups: a control (C, 0.1 ml 0.9% saline), buflomedil (B, 3 mg/kg/day), and pentoxifylline group (P, 14.5 mg/kg/day). Treatments administered intraperitoneally were initiated 1 hour before skin flap preparation and continued for 7 days post-operatively at 12-hour intervals. Preparations (skin flaps) were divided into 12 fields, which were organized into six bands. Functional capillary density (FCD, in mm/mm²), distance from the skin flap base to blood flow cessation (Dist with flow, in cm), percentage of viable skin (VA, in%), and qualitative analysis of blood flow by orthogonal polarization spectral imaging were performed at 1 and 24 hours and on the seventh post-operative day. RESULT: Bands IV, V, and VI presented no flow independent of time. The functional capillary density group B was higher than that of groups C and P, primarily after 24 hours. All groups showed an increase in D with time but reached similar final distances (C = 2.73, B = 2.78 and P = 2.70 cm). Moreover, the percentage of viable areas remained at approximately 50%. The orthogonal polarization spectral imaging was useful to assess viability by counting fields with and without blood flow. CONCLUSIONS: Functional capillary density values were higher in the buflomedil group compared to the control and pentoxifylline groups in this model. Functional capillary density did not influence D or the percentage of VA, and the technique showed favorable potential to assess/predict the viability of skin flaps within 1 h after surgery.