Resumo Objetivou-se avaliar os efeitos dos manejos do pastejo ponta e repasse combinados a substituição parcial da proteína do suplemento por nitrogênio ureico sobre o consumo e digestibilidade dos nutrientes, composição do leite e balanço de nitrogênio de vacas primíparas da raça Girolanda. As vacas foram alocadas em dois quadrados latinos 4 x 4 compostos de quatro animais cada e quatro tratamentos em um esquema fatorial 2 x 2. Os suplementos foram formulados para um consumo de 0,6% do peso corporal: sem utilização de nitrogênio ureico; ou com 21% de nitrogênio ureico em sua composição. O manejo do pastejo de ponta melhorou o consumo e a digestibilidade dos nutrientes. O suplemento contendo ureia excretou 47% a mais de N urina (g/dia) em comparação ao suplemento sem ureia. A combinação entre o suplemento sem ureia e o manejo de pastejo ponta apresentou maior ingestão e retenção de nitrogênio e maior N retido (%N digerido). A combinação entre o suplemento contendo 21% de nitrogênio ureico e o pastejo de ponta pode ser utilizada sem prejuízos aos parâmetros nutricionais e produtivos das vacas.

Abstract This study aimed to evaluate the effect of the first and last stocking strategies combined with a partial substitution of the protein from the supplement for urea nitrogen on nutrient intake and digestibility, milk composition, and nitrogen balance of primiparous Girolando cows. The cows were allocated to a double 4 × 4 Latin square composed of four animals and four treatments in a 2 × 2 factorial arrangement. Supplements were formulated to provide an intake of 0.6% body weight, with and without the inclusion of 21% urea nitrogen in their composition. The first stocking management method improved nutrient intake and digestibility. Supplementation with urea led to a 47% higher excretion of urine N (g/day) than the urea treatment. The combination of the supplement without urea and the first stocking provided higher intake and retention of nitrogen and higher retained-N levels (%digested N). The combination of a supplement containing 21% urea nitrogen and the first stocking can be used without compromising the nutritional and productive parameters of the cows.

Abstract Background: Gene expression alterations have been implicated in suicide pathology. However, the study of the regulatory effect of DNA methylation on gene expression in the suicidal brain has been restricted to candidate genes. Objective: The objective of the study was to identify genes whose expression levels are correlated with DNA methylation in the prefrontal cortex of suicides. Methods: Postmortem prefrontal cortex samples from 21 suicides and six non-suicides were collected. Transcriptomic and DNA methylation profiles were evaluated with microarrays; cis correlations between gene expression and CpG methylation were screened. We then analyzed the presence of transcription factor (TF) binding sites (TFBS) at CpG sites correlated with gene expression. Gene expression of TFs involved in neurodevelopmental binding to predicted TFBS was determined in the BrainSpan database. Results: We identified 22 CpG sites whose methylation levels correlated with gene expression in the prefrontal cortex of suicides. Genes annotated to identified CpG sites were involved in neurodevelopment (BBS4, NKX6-2, AXL, CTNND1, and MBP) and polyamine metabolism (polyamine oxidase [PAOX]). Such correlations were not detected in the non-suicide group. Nine TFs (USF1, TBP, SF1, NRF1, RFX1, SP3, PKNOX1, MAZ, and POU3F2) showed differential expression in pre- and post-natal developmental periods, according to BrainSpan database. Conclusions: The integration of different omic technologies provided novel candidates for the investigation of genes whose expression is altered in the suicidal brain and their potential regulatory mechanisms. (REV INVEST CLIN. 2020;72(5):283-92)

Bipolar disorder (BD) is a chronic mental illness characterized by changes in mood that alternate between mania and hypomania or between depression and mixed states, often associated with functional impairment. Although effective pharmacological and non-pharmacological treatments are available, several patients with BD remain symptomatic. The advance in the understanding of the neurobiology underlying BD could help in the identification of new therapeutic targets as well as biomarkers for early detection, prognosis, and response to treatment in BD. In this review, we discuss genetic, epigenetic, molecular, physiological and neuroimaging findings associated with the neurobiology of BD. Despite the advances in the pathophysiological knowledge of BD, the diagnosis and management of the disease are still essentially clinical. Given the complexity of the brain and the close relationship between environmental exposure and brain function, initiatives that incorporate genetic, epigenetic, molecular, physiological, clinical, environmental data, and brain imaging are necessary to produce information that can be translated into prevention and better outcomes for patients with BD.

Abstract The purpose of this study was to examine the growth and senescence dynamics of Digitaria eriantha cv. Survenola as a response to several canopy heights (25, 35, 45, and 55 cm) of harvests in two seasons of the year (rainy and dry). The experiment was established at the Federal University of Sergipe, in São Cristóvão - SE, Brazil, from 03/26/2015 to 03/28/2016, under a randomized-complete-block design with four replicates. Plots were irrigated, and upon reaching the pre-established canopy heights, they were cut to a 10 cm-residual height. Increasing canopy heights in the dry season led to lower tiller density, for basal and aerial tillers. However, irrespective of tiller type and season, this variable increased after the rest period. Light interception rose with canopy heights (P<0.05) in the dry period and it showed a quadratic response in the rainy season. Greater canopy heights led to a higher (P<0.05) daily leaf senescence rates and daily stem growth rates. Regardless of the season, the total accumulation and net herbage accumulation rates of basal tillers increased as canopy heights did. The greatest canopy height promoted the daily stem production, whereas the lowest height did not allow the grass to express its production potential. The defoliation-height range of 35 to 45 cm is the most recommended, as it favors the control of stem growth and leaf senescence.

Resumen El objetivo del estudio fue conocer la dinámica del crecimiento y senescencia del forraje de Digitaria eriantha Steud. cultivar Survenola al alcanzar diferentes alturas de corte (25, 35, 45 y 55 cm), en dos épocas del año (lluvias y seca). El experimento se condujo en la Universidad Federal de Sergipe, en San Cristóbal-Sergipe, entre el 26/03/2015 y el 28/03/2016, con un diseño de bloques completos aleatorizados y cuatro repeticiones. Las unidades experimentales fueron irrigadas, y al alcanzar las plantas las alturas preestablecidas, se cortaron a 10 cm sobre el nivel del suelo. Las dos alturas de rebrote mayores redujeron en la época seca la densidad poblacional de tallos basales y aéreos; sin embargo, independientemente del tipo de tallo y la época del año, la densidad de población de tallos aumentó durante el período de descanso. La intercepción de luz en la época seca se incrementó (P<0.05) en las alturas de corte mayores, y en la época de lluvias su comportamiento fue cuadrático. Las alturas de corte mayores aumentaron (P<0.05) la tasa diaria de senescencia de las hojas y la tasa diaria de crecimiento de los tallos basales y aéreos. Las tasas de acumulación total y acumulación neta de forraje de tallos basales, independientemente de las épocas, tuvieron una relación positiva con el incremento en las alturas de corte. La mayor altura de corte produjo la mayor producción diaria de los tallos; además, la menor altura de corte no permitió a la gramínea expresar su potencial productivo. El intervalo de defoliación entre las alturas de 35 a 45 cm es el más indicado, pues favorece el control en el crecimiento del tallo y la senescencia de las hojas.

ABSTRACT. The objective of this study was to evaluate the activity of invertases and amylases in Brachiaria brizantha cv. Marandu under various shade and nitrogen fertilization conditions. The experiment was carried out in a greenhouse using a 4 x 2 factorial scheme (shading levels of 0, 30, 50, and 80% and fertilization with 0 and 100 kg N ha-1). The activity of the enzymes, cytosol-neutral invertase (Inv-N), vacuole acid (Inv-V) and cell-acidic acid (Inv-CW), reducing sugars (RS), and α and β-amylases were evaluated (α = 0.05). The interaction was significant for Inv-N within the leaf. In the first cycle, the highest activity was in fertilized plants with 30, 50, and 80% shading. For Inv-CW in the 1st cycle, the highest activity occurred with 0, 30, and 50% shading. However, the interaction for Inv-V leaf activity was not significant in the 1st and 2nd cycles. The highest activity observed for Inv-V was in the fertilized plants, suggesting that fertilization increased the enzymatic activity. The activity of the invertases increased both under 30-50% shaded conditions and in full sun. Furthermore, invertase activity was directly linked to the osmoregulatory system. The reduction in RS was related to a low photosynthetic rate, and an increase α and β-amylase was associated with the use of reserve energy sources to meet energetic needs.

Resumen Existe una preocupación por la caracterización química de los alimentos con potencial económico y nutricional, en especial los de bajo valor calórico, en su constitución de proteínas, lípidos, carbohidratos, minerales y vitaminas, para una alimentación sana. Entre esos alimentos, las flores comestibles se destacan por su belleza y condimentación en los platos; además, poseen propiedades nutricionales y medicinales. En este contexto, el objetivo del trabajo fue caracterizar químicamente los pétalos de las especies de rosa (Rosa x grandiflora Hort.), girasol (Helianthus annuus L.) y caléndula (Calendula officinalis L.), para el uso en la alimentación humana. Las plantas fueron cultivadas en ambiente protegido y cielo abierto, y después de la cosecha se realizaron los análisis físico-químicos (carbohidratos, cenizas, extracto etéreo, humedad, fibra y proteína). En el presente estudio se observó un alto contenido de agua (> 80 %); el girasol tuvo mayor contenido de cenizas (1,2 %); la caléndula tuvo mayor contenido de extracto etéreo (1,2 %), y la rosa tuvo mayor contenido de fibra (3,2 %). Los pétalos de las flores de caléndula, girasol y rosa presentaron una composición química con nutrientes esenciales, pudiendo ser incluidos en los menus diarios por contener nutrientes esenciales para una dieta sana.

Abstract There is a concern for the chemical characterization of foods with economic and nutritional potential, especially those of low caloric value, in their constitution of proteins, lipids, carbohydrates, minerals, and vitamins, important for a healthy diet. Among these foods, edible flowers are distinguished for their beauty and for flavoring dishes; furthermore, they also have nutritional and medicinal properties. In this context, the aim of the study was to characterize chemically the petals of three species, rose (Rosa x grandiflora Hort.), sunflower (Helianthus annuus L.), and calendula (Calendula officinalis L.) for use in human nutrition. The plants were cultivated in a protected environment and in an open field, and after harvest, physicochemical analyses were carried out (ash, carbohydrates, ethereal extract, fiber, moisture, and protein). In the current study, high water content was observed (higher than 80 %); sunflower showed a higher ash content (1.2 %), calendula presented a higher ethereal extract content (1.2 %) and rose had a higher fiber content (3.2 %). Rose, sunflower and calendula flower petals had a chemical composition with essential nutrients, being able to be included in the daily human diet because they contain essential nutrients for a healthy diet.

RESUMO Neste estudo investigamos se o movimento e distribuição do peixe migrador, Salminus brasiliensis, são afetados por um pequeno reservatório. Também avaliamos como movimento e distribuição se relacionam com a temperatura da água, fluxo e pluviosidade. Em dezembro de 2011 e janeiro 2012, 24 indivíduos foram capturados a jusante da barragem, marcados com radiotransmissores e liberados no reservatório (5,46 km²). Depois de soltos, 18 dos 24 peixes marcados se deslocaram para montante, levando em média 16,6 dias para ultrapassar o reservatório com movimentos diários variando de < 5km/dia a > 24km/dia. Entretanto, apenas sete (29,16%) dos peixes marcados retornaram para seção inferior do reservatório, a maior parte mantendo-se nas seções intermediárias e superiores do reservatório. Distribuição longitudinal e movimento dos peixes foram relacionados positivamente com o fluxo de montante dentro do reservatório e com a temperatura da água. Assim, encontramos evidências que S. brasiliensis pode reconhecer o gradiente longitudinal e continuar sua migração ascendente. Por outro lado, nossos resultados também indicam que o movimento para jusante foi afetado, possivelmente, devido à desorientação, o que impede que os indivíduos alcancem as seções inferiores do reservatório. Com base nos resultados, sugerimos que impactos sobre peixes migratórios devem ser objetivamente abordados em avaliações ambientais e gestão ambiental de pequenos reservatórios.

ABSTRACT We investigated whether the movement and distribution of the migratory fish, Salminus brasiliensis, were affected by a small reservoir. We also examined how movement and distribution were related to water temperature, flow, and rainfall. In December 2011 and January 2012, 24 individuals were captured downstream from the dam, tagged with radio transmitters and released in the reservoir (5.46 km² total area). After being released in the reservoir, 18 of the 24 tagged fish travelled upstream, taking an average of 16.6 days to leave the reservoir, with daily movements varying from < 5 km/day to > 24 km/day. However, only seven tagged specimens (29.16%) returned to the lower reservoir section, while the rest remained in the intermediate and upper reservoir sections. Longitudinal distributions and movements were positively related to both upstream flow into the reservoir and water temperature. We found evidence that S. brasiliensis can recognize the longitudinal gradient and is able to continue its upstream migration. On the other hand, the reservoir negatively affected downstream movements, possibly because disorientation prevented movement to the lower reservoir section. Based on these results, we suggest that the impact of small reservoirs on migratory fish species should be objectively addressed in environmental impact assessments and management programs.

ABSTRACT. The objective of this study was to verify the activity of invertases in Estilosantes under the influence of cultivation systems and nitrogen fertilization in two seasons. The experiment was conducted in the Xaraés area, where Estilosantes was planted according to a 2 x 2 factorial scheme with two cultivation systems (monoculture and mixed pasture) in the absence or presence of fertilization (75 kg of N ha-1). A randomized block design with four replications was used. The neutral cytosol (Inv-N), vacuole acid (Inv-V) and cell wall acid (Inv-CW) invertase activity and reducing sugar contents in the leaves and stems were evaluated and analyzed by a 5% probability F-test. The interaction between cultivation systems and nitrogen fertilization was significant for Inv-N in the leaves in the summer, such that greater activity occurred in response to the mixed pasture system and nitrogen fertilization. Nitrogen increased the activity of the Inv-N in the stem in both periods, increased the activity of Inv-V in the leaf in the summer, and decreased the activity of Inv-CW in the fall. This cultivation system influenced the activity of Inv-N regardless of the period of the year and did not interfere with the activities of the acid invertases. The invertases did not show regular changes in relation to nitrogen fertilization.

RESUMO A reprodução de peixes em rios com planície de inundação está associada ao regime de vazão e sua interação com os hábitats laterais. Entretanto, em rios confinados, sem planícies de inundação, pode-se esperar que a relação entre reprodução e vazão seja comparativamente distinta. Neste estudo, descrevemos as características reprodutivas da história de vida de Hemiancistrus fuliginosus e Hypostomus isbrueckeri em um rio confinado, discutimos as diferenças em relação à reprodução de loricarídeos em planícies de inundação e também as implicações para o efeito de barragens e manejo da vazão de rios. O pico reprodutivo de ambas as espécies ocorreu durante o período de baixa vazão, logo após o período de máxima vazão, contrastando com o padrão observado na reprodução de espécies em planícies de inundação, que ocorre principalmente no período de incremento de vazão. As duas espécies apresentaram história de vida com estratégia de equilíbrio. Pelo fato de ambas espécies desovarem durante a baixa vazão do rio, cuja ocorrência no verão é historicamente previsível, sugerimos que seus padrões de desova podem ser severamente alterados, dependendo de como o regime de vazão for afetado pela operação da barragem. Esses resultados têm implicações para avaliação e manejo de impactos ambientais de barragens em rios confinados, sendo que o manejo ecológico de vazão e a conservação de rios livres de barragens como alternativas de mitigação e conservação, respectivamente.

ABSTRACT Fish reproduction in floodplain rivers is often linked to flow regime and with the inundation of floodplain habitats. However, in confined rivers, where floodplains are absent, the relation between reproduction and flow can in comparison be expected to be distinct. In this study, we describe the reproductive life-history of Hemiancistrus fuliginosus and Hypostomus isbrueckeri in a confined river and discuss its differences relative to floodplain loricariids and also the implications for effects of dam construction and flow regulation. We found the reproductive peak of both species occurred during lowering waters, just after maximum river flow, in contrast to floodplain species that tend to spawn during rising waters. The studied species presented attributes of equilibrium life-history strategy, which are related to predictable river flow variation. Because both species spawned during low river flow, which is historically predictable in summer, we suggest that their reproduction may be severely disrupted, depending on how flow regime is affected by dam operation. These results have implications for assessing and mitigating the impacts of river damming on fish populations in confined rivers, and we point to ecologically driven flow management and conservation of free-flowing rivers as mitigation and conservation alternatives.

Resumo Objetivos: Avaliar se há associações entre a IL-6, o TNF-α, a IL-10 e a estado nutricional em pacientes com cirrose secundária a atresia biliar e comparar com controles saudáveis. Métodos: Os parâmetros usados na avaliação nutricional foram desvio padrão de estatura para a idade e espessura da prega cutânea do tríceps para a idade. A gravidade da cirrose foi avaliada por meio da classificação de Child-Pugh e do PELD/MELD. As citocinas no soro foram medidas por citometria de fluxo – técnica de Cytometric Bead Array. Resultados: A IL-6, o TNF-α e a IL-10 foram significativamente maiores no grupo de cirrose em comparação com o grupo de controle [2,4 em comparação com 0,24 (p < 0,001)], [0,21 em comparação com 0,14 (p = 0,007)] e [0,65 em comparação com 0,36 (p = 0,004)], respectivamente. A IL-6 e a IL-10 demonstraram correlação positiva com a gravidade da doença (0,450; p = 0,001) e (0,410; p = 0,002), respectivamente. O TNF-α não mostrou relevância na gravidade da doença (0,100; p = 0,478). Com relação à avaliação nutricional, a IL-6 demonstrou correlação negativa com o desvio padrão de estatura para a idade (−0,493; p < 0,001) e o desvio padrão de espessura da prega cutânea do tríceps para a idade (−0,503; p < 0,001), respectivamente. A IL-10 demonstrou correlação negativa com o desvio padrão de estatura para a idade (−0,476; p < 0,001) e o desvio padrão de espessura da prega cutânea do tríceps para a idade (−0,388; p = 0,004), respectivamente. O TNF-α não mostrou relevância em ambos os parâmetros antropométricos [(−0,083; p = 0,555); (−0,161; p = 0,253)]. Conclusão: Assim, sugerimos que, em pacientes com cirrose secundária a atresia biliar, IL-6 pode ser usado como um possível biomarcador de suporte do estado nutricional deficiente e níveis aumentados de IL-10 podem ser usados como um possível biomarcador de suporte, em fase inicial, de deterioração do estado nutricional.

Abstract Objectives: The objective of the present study is to evaluate whether IL-6, TNF-α, IL-10 are associated with nutritional status in patients with cirrhosis secondary to biliary atresia and compare to healthy controls. Methods: The parameters used for nutritional assessment were the standard deviation scores of height-for-age and of triceps skinfold thickness-for-age. The severity of cirrhosis was evaluated using the Child–Pugh score and PELD/MELD. Serum cytokines were measured using Cytometric Bead Array flow cytometry. Results: IL-6, TNF-α, and IL-10 were significantly higher in the cirrhosis group when compared with the control group (2.4 vs. 0.24 (p < 0.001), 0.21 vs. 0.14 (p = 0.007), and 0.65 vs. 0.36 (p = 0.004), respectively. IL-6 and IL-10 were positively correlated with disease severity (0.450 [p = 0.001] and 0.410; [p = 0.002], respectively). TNF-α did not show a significant correlation with disease severity (0.100; p = 0.478). Regarding nutritional evaluation, IL-6 was negatively correlated with the standard deviation score of height-for-age (−0.493; p < 0.001) and of triceps skinfold thickness-for-age (−0.503; p < 0.001), respectively. IL-10 exhibited a negative correlation with the standard deviation score of height-for-age (−0.476; p < 0.001) and the standard deviation score of triceps skinfold thickness-for-age (−0.388; p = 0.004). TNF-α did not show any significance in both anthropometric parameters (−0.083 (p = 0.555) and −0.161 (p = 0.253). Conclusion: The authors suggest that, in patients with cirrhosis secondary to biliary atresia, IL-6 could be used as a possible supporting biomarker of deficient nutritional status and elevated IL-10 levels could be used as a possible early-stage supporting biomarker of deteriorating nutritional status.