ABSTRACT Purpose To evaluate the effect of creatine supplementation in the diet of rats subjected to ischemia and reperfusion of hind limbs. Methods Eighteen male Wistar rats were randomized to receive dietary creatine supplementation (G1) or no supplementation (G2), before being subjected to 4 h of ischemia followed by 4 h of reperfusion. In addition, 10 rats (G3) underwent the same surgical procedure, without ischemia, but with supplementation. After reperfusion, kidney and musculature were evaluated for histological damage and serum levels of alanine aminotransferase, urea and creatinine were obtained. Results The urea dosage showed significant differences between the groups (averages G1 = 155.1; G2 = 211.27; G3 = 160.42). Histological analysis found significant differences between G1 and G2 (but not between G1 and G3) in renal myoglobin cylinders and vacuolar degeneration variables and in hypereosinophilia and karyopyknosis variables in muscle fibers. There were no significant differences in the other variables studied. Conclusions Creatine supplementation was related to fewer histological lesions, as well as lower levels of plasma urea, which may suggest a protective effect against lesions caused by ischemia and reperfusion of posterior paws muscles in Wistar rats.

OBJETIVO: Investigar o efeito do cilostazol no rim e na musculatura esquelética de ratos submetidos à isquemia aguda e reperfusão. MÉTODOS: Quarenta e três animais foram aleatoriamente distribuídos em dois grupos. Grupo I recebeu solução de cilostazol (10 mg/Kg) e Grupo II recebeu solução fisiológica a 0,9% (SF), após ligadura da aorta abdominal. Decorridas quatro horas de isquemia os animais foram distribuídos em quatro subgrupos: Grupo IA (Cilostazol): duas horas de reperfusão. Grupo IIA (SF): duas horas de reperfusão. Grupo IB (Cilostazol): seis horas de reperfusão. Grupo IIB (SF): seis horas de reperfusão. Após a reperfusão, realizou-se nefrectomia esquerda e a retirada da musculatura de membro posterior. Os parâmetros histológicos estudados em rim foram cilindros de mioglobina, degeneração vacuolar e necrose tubular. Em músculo foram edema, infiltrado inflamatório, hipereosinofilia de fibras, cariopicnose e necrose. A apoptose foi avaliada por imunohistoquímica, através da caspase-3 clivada e TUNEL. RESULTADOS: Não houve diferença estatisticamente significante entre os grupos estudados. CONCLUSÃO: O cilostazol não teve efeito protetor sobre o rim e sobre a musculatura estriada esquelética em ratos Wistar submetidos à isquemia aguda e reperfusão no modelo estudado.

PURPOSE: To investigate the effect of cilostazol, in kidney and skeletal muscle of rats submitted to acute ischemia and reperfusion. METHODS: Fourty three animals were randomized and divided into two groups. Group I received a solution of cilostazol (10 mg/Kg) and group II received saline solution 0.9% (SS) by orogastric tube after ligature of the abdominal aorta. After four hours of ischemia the animals were divided into four subgroups: group IA (Cilostazol): two hours of reperfusion. Group IIA (SS): two hours of reperfusion. Group IB (Cilostazol): six hours of reperfusion. Group IIB (SS) six hours of reperfusion. After reperfusion, a left nephrectomy was performed and removal of the muscles of the hind limb. The histological parameters were studied. In kidney cylinders of myoglobin, vacuolar degeneration and acute tubular necrosis. In muscle interstitial edema, inflammatory infiltrate, hypereosinophilia fiber, cariopicnose and necrosis. Apoptosis was assessed by immunohistochemistry for cleaved caspase-3 and TUNEL. RESULTS: There was no statistically significant difference between groups. CONCLUSION: Cilostazol had no protective effect on the kidney and the skeletal striated muscle in rats submitted to acute ischemia and reperfusion in this model.

O objetivo deste estudo foi investigar o uso dos adesivos etil-cianoacrilato e butil-cianoacrilato com o náilon monofilamentar, comparativamente , na síntese da pele de ratos. Trinta e dois ratos foram distribuídos em três grupos: A, B e C. Estes grupos foram divididos em dois subgrupos, para estudo no sétimo e no décimo-quarto dia pós-operatório. Foram efetuadas no abdome do rato, duas incisões longitudinais e paralelas distando cada uma delas um centímetro da linha mediana, sendo uma do lado direito e a outra do lado esquerdo. Em todos os grupos a síntese da incisão do lado direito, foi realizada com pontos separados de náilon 5-zeros, sendo este considerado o grupo controle (C). Nos animais do grupo A, a síntese do lado esquerdo foi realizada com etil-cianoacrilato (Super Bonder) e nos animais do grupo B, a síntese foi realizada com butil-cianoacrilato (Histoacryl). No sétimo e no décimo-quarto dia pós-operatório, o aspecto macroscópico da cicatriz não mostrou diferença significante.No aspecto microscópico.os grupos B e C foram melhores do que o A. Os resultados demonstraram que a síntese com o adesivo sintético é eficaz, mais rápida, indolor e com bons resultados estéticos.

Cyanoacrylate derivates have been used as surgical adhesives, like ethyl-cyanoacrylate(Super-Bonder) and buthyl-cyanoacrylate(Histoacryl) for many years.In this study, the histotoxicity and binding ability of Super-Bonder and Histoacryl were compared.32 rats were operated, and distributed in 3 groups :A, B and C. Each group was subdivived in 2 subgroups , to be studied after 7 and 14 days postoperatory .2 longitudinals incisions with 3 centimetre in lenght, were done in each animal. The left incision was sutured with Nylon 5.0 (group C) as controls and the right incision was sutured with ethil-cyanoacrylate (group A) and buthil-cyanoacrylaty (group B). Our results allowed us to clearly study tissue acute inflammation, the presence of dehiscense and fibrosis that eventualy occured. Throughtout the study period, there was no statistical difference in the viability of buthyl-cyanoacrylate and Nylon. Buthyl-cyanoacrylate showed mild acute inflammation, and mild foreign body giant cell reaction, and had minimal histotoxic effect when compared with ethyl-cyanoacrylate. There was non intolerance to the adhesive nor dehiscense. This method is easy and without side effects on the skin.

O objetivo da pesquisa foi estudar a eficácia do enxerto autógeno de fáscia lata, para tratar ferimentos penetrantes provocados no esôfago cervical de cães. Foram estudadoscomparativamente dois procedimentos operatórios para se corrigir lesões orificiais provocadas na parede do esôfago cervical do cão com uma área padronizada de 3,12 cm2 Foram operados 40 cães, divididos em 2 grupos iguais denominados, grupo A (sutura ) e grupo B (enxerto) .Cada grupo foi subdividido em dois sub grupos denominados A1, A2 e B1, B2, com 10 animais cada para serem reoperados no 7<FONT FACE="Symbol">°</FONT> e no 30<FONT FACE="Symbol">°</FONT> P.O.respectivamente.No grupo controle, (A ) foi aplicada a sutura em plano único extramucosa com pontos simples de náilon 5-zeros .No grupo experimento (B) aplicou-se para ocluir o defeito provocado um enxerto de fáscia lata, suturado à borda da parede do esôfago com pontos simples extramucosa em plano único, com fio de náilon 5-zeros. Comparou-se os achados macroscópicos e microscópicos dos dois grupos não se observando diferença estatística significante, mas quanto ao aspecto clínico, os animais do grupo A apresentaram resultados melhores no que se refere a formação de fístulas apresentando um caso, enquanto o grupo B apresentou cinco casos Concluimos que a fáscia lata não é eficaz para tratar ferimentos penetrantes no esôfago cervical de cães.

The aim of this study was to study the efficiency of autogenus graft of fascia lata to treat experimental wounds at the cervical level of esophagus of dogs. Two operative procedures were used in forty dogs in order to correct the injuries and compared among the groups. The standard area of the injury ranged was 3,12 cm square. The animals were divided in two groups of 20 each, so-called Group A ( suture group ) and Group B ( graft groups ). Group A and B were subdivided in two another group, so-called Group A1 A2 and B1,B2 with 10 animals each one with the pourpose of reoperation at seventh and thirtieth past operatives days. The Group A, so-called Control Group was undergone a techinique of extramucous suture with simple points of nylon on transversal plan of esophagus axis. On Group B, so-called Experimental Group it was employed autogenos graft of fascia lata in order to oclud the penetrating injury. The grafts was sutured on the esophagus wal by extramucous technique with the nylon . Macroscopic and microscopic results were compared among the two groups by invariate analysis. No statistical significant differences were observed among the groups besides the animals of Group A had showed a good clinical behavior and lower incidence of fistulae. However on Group B ( undergone a fascia lata graft ) had shouwed five cases of fistulae. In conclusion, the fascia lata not appear to be useful for treat penetrating wounds at the cervical level of esophagus in dogs.