Abstract Lippia origanoides Kunth has among its synonyms Lippia sidoides Cham. and is popularly known as ‘alecrim-pimenta’ or ‘rosemary pepper’. Essential oil of L. origanoides (EOLO) has been previously reported for several biological activities without significant acute toxicity, even at higher doses. In this study, essential oil (EO) was extracted from dried leaves, inflorescences, and branches of L. origanoides from Limoeiro do Norte, Ceará, Brazil by hydrodistillation at 3.63% (w/w) yield. Posteriorly, the presence of chemical compounds in the EOLO was identified by gas chromatography coupled to mass spectrometry (GC-MS). The analysis allowed to identify the thymol as the main metabolite (83.53%), followed by p-cymene (5.92%), caryophyllene (4.31%), γ-terpinene (3.27%), thymol methyl ether (1.18%), β-myrcene (1.00%), and α-terpinene (0.79%). The minimal inhibitory concentration (MIC) of EOLO was assessed against Gram-negative and Gram-positive bacterial pathogens such as Enterobacter aerogenes, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella enterica serovar Typhimurium, and Staphylococcus aureus. At 10 µL/mL concentration, the EOLO exhibited antibacterial activity against all the tested bacterial pathogens, and this concentration was considered as the MIC. The minimum bactericidal concentration (MBC) of EOLO was assessed at the MIC concentration, which inhibited the complete growth of all tested bacterial pathogens after 3 h. Therefore, concentrations less than 10 µL/mL were set as MBC. Thus, our findings suggest that the EOLO can be used as a promising antibacterial agent in the industries.

Resumo: Este artigo analisa a trajetória tecnológica do cultivo de camarão no Nordeste brasileiro e suas implicações ou limitações para a adoção de inovações sustentáveis ambientalmente. Desse modo, uma abrangente pesquisa empírica foi realizada, usando dados secundários e entrevistas semiestruturadas com vários agentes envolvidos no cultivo do camarão. Assim, foi possível identificar o perfil desse setor de atividade com base em sua trajetória de desenvolvimento desde o início de sua produção no Brasil. Os principais resultados do estudo apontam que a trajetória tecnológica dessa indústria revelou limitações, mas, também, algumas oportunidades para a geração de inovações sustentáveis, especialmente no estágio atual.

Abstract: This paper analyzes the technological route of shrimp farming in Northeast Brazil and their implications or limitations for the adoption of environmentally sustainable innovations. Thus, an extensive empirical research was performed, using secondary data and semi-structured interviews with various agents involved in shrimp farming industry. Therefore, it was possible to identify the profile of this activity based on its development trajectory since the beginning of its production in Brazil. The main results of this study indicate that the technological path of this industry revealed limitations, but also some opportunities for the generation of sustainable innovation, especially at the current stage.

Resumo A lagosta é uma espécie marinha de grande importância em muitos países. As mudanças bioquímicas e microbiológicas que ocorrem após a captura determinam sua qualidade e vida útil. Objetivou-se, neste trabalho, estudar o efeito da temperatura de armazenamento sob congelamento (-30 ºC, -80 ºC e -150 ºC), do tipo de embalagem (polietileno de baixa densidade – PEBD, com vácuo e sem vácuo) e do tempo de armazenamento (0, 60, 120, 180, 240, 300 e 360 dias) sobre as características físicas de cor (luminosidade, intensidade de vermelho e amarelo) e químicas (proteínas e lipídeos) da lagosta. Durante o armazenamento, os parâmetros físicos luminosidade e intensidade de vermelho aumentaram, a intensidade de amarelo diminuiu, os teores de proteína diminuíram, porém para lipídeos não houve diferença significativa. Pode-se concluir que as temperaturas de armazenamento estudadas e a presença ou ausência de vácuo nas embalagensforam eficientes na manutenção das características físicas e químicas das caudas das lagostas armazenadas.

Abstract Lobster is a marine species of great importance in many countries and the biochemical and microbiological changes that occur after capture determine its quality and shelf life. The objective of this work was to study the effect of the storage temperature (-30 ºC, -80 ºC and -150 ºC), the type of packaging (low density polyethylene - LDPE with and without vacuum) and the storage time (0, 60, 120, 180, 240, 300 and 360 days) on the colour (luminosity, red and yellow intensity) and chemical characteristics (proteins and lipids) of the lobster. During storage the physical parameters of luminosity and red intensity increased, the yellow intensity decreased, the protein levels decreased and there was no significant difference in the lipid content. It was concluded that the freezing temperatures studied and both the presence and absence of vacuum in the polyethylene bags during storage were efficient in maintaining the physical and chemical characteristics of the lobster tails.

Abstract This study aimed at determining the centesimal composition, the energy value and the sodium content in beef bouillon cubes and compare them with the nutritional information by the manufacturer. Beef bouillon cube samples were collected in supermarkets in of the city of Limoeiro do Norte/CE, and the levels of nutrients were determined by official analysis methods. The physicochemical analyses carried out were moisture, lipids, proteins, fixed mineral residue, total carbohydrates and sodium content. The beef bouillon cubes analyzed showed a low moisture content (3.23 – 4.23 g/100 g) and high levels of lipids (19.72 – 20.05 g/100 g) and fixed mineral residue (53.24 – 57.61 g/100 g). When compared to the data reported on their labels, all the parameters were in smaller amounts than the ones declared in all analyzed brands. The average sodium content found in the samples ranged from 758.5 to 815.5 mg/serving of the product. Considering that the results were based on a portion of broth and that these amounts are related to half a cube of beef bouillon, it becomes quite alarming the amount of sodium available in this type of product, since the food prepared with this ingredient is usually eaten alongside other foods, which also contain sodium in their composition.

Abstract The objective of this study was to elaborate and evaluate the shelf life of the liquid artisanal demi-glace sauce considering sensory, microbiological and lipid oxidation characteristics during 75 days of storage compared to commercial hydrated demi-glace sauce. Sensory aspects (visual and olfactory), counts of coliform and Staphylococcus aureus, determination of Salmonella sp. and lipid oxidation were evaluated every 15 days. The artisanal demi-glace sauce was homogenous, without sensory alteration during the storage period, with olfactory alteration only at the end. The commercial demi-glace sauce remained unchanged until t3 (30 days), from t4 (45 days) presented turbid appearance, followed by phase separation and with unpleasant odor in t6 (75 days). The lipid oxidation showed an increased concentration of malonaldehyde at every evaluated time. The artisanal and commercial demi-glace sauces showed a significant difference at t1, t2, t4 and t5, when the artisanal sauce had lower contents of malonaldehyde (18, 23, 36 and 11%, respectively), only at the 30th and 75th days of storage there was significant similarity. The sauces were within the microbiological standards required by the law. Although the artisanal demi-glace sauce does not contain conservatives, its shelf life was similar to the hydrated commercial product.