CoCrMoSi alloys microstructure is affected by processing and their properties have been associated with the high volume fraction of Laves phase. However, not much has been reported on the effect of structure refinement and morphology on the properties of these alloys. This paper evaluates a CoCrMoSi alloy processed by centrifugal casting and Plasma Transferred Arc (PTA) hardfacing, aiming to understand the correlation between microstructure and properties at room temperature in the as-processed condition and following exposure at 600 °C. Characterization was carried out using scanning electron microscopy, X-ray diffraction, hardness and wear tests. The cooling rates associated with each of the processing techniques used account for the developed microstructures and associated hardness, a 626 HV0,5 cast alloy and 649 HV0,5 deposited alloy, the former exhibiting a coarser microstructure. Wear behavior was determined by the dispersion of solid solution areas in the microstructure and as a consequence temperature exposure aggravated the poorer wear resistance of the cast alloy. The finer phase dispersion formed in coatings overlapped the deleterious effects of dilution and account for the stable wear behavior after temperature exposure.

Steel sheet coated with zinc or 55%Al-Zn is widely used at the civil construction, mainly for roofing and cladding applications. The 55%Al-Zn coated steel shows mechanical properties slightly different from zinc coated steel, even under the same heat cycle processing, due to the higher molten metal temperature and also to the higher strength of the 55%Al-Zn coating. The knowledge of those differences can be used by the final users to evaluate if some of those products are not suitable for any specific application. Although those two coated products are widely used at the civil construction in Brazil, only a limited literature is available on the difference between the mechanical properties of those products. Through tensile tests of commercial grade samples - as coated and after removing the coating - the aim of this paper is to evaluate the mechanical properties (tensile strength, yield strength, total elongation and hardening coefficient) and also how much the coating influences the mechanical properties of the zinc and 55%Al-Zn coated steel. The 55%Al-Zn coated steel showed higher yield and tensile strength and lower total elongation and hardening coefficient compared to zinc coated steel and the coating showed a significant influence on the mechanical properties of both products.

Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa epsilon-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera.