ABSTRACT Objective to determine the microbiological profile of bacteria isolated and identified from beds and infusion pumps in the intensive care unit of a university hospital in Rio de Janeiro state. Method nine samples were collected from patients’ bed side rails and eight from infusion pump keypads in an intensive care unit in October 2014. An area of 100cm2 was delimited as the sampling parameter. Samples were collected using sterile swabs, which were wetted and transported with Cary-Blair. The microorganisms were isolated, classified, and then tested for antimicrobial resistance. Results coagulase-negative Staphylococcus was the most prevalent type. Antimicrobial susceptibility testing indicated some of these Staphylococci were multi-drug resistant. Conclusion multi-professional discussion of hospital safety issues must be expanded, and continuing professional development emerges as one possible pathway to success in nosocomial infection control.

RESUMEN Objetivo determinar el perfil microbiológico de bacterias aisladas e identificadas en las camas y las bombas de infusión en la unidad de terapia intensiva de un hospital universitario de Rio de Janeiro. Método se recolectaron nueve muestras de rejas de camas de pacientes y ocho de paneles de las bombas de infusión de una unidad de terapia intensiva, en octubre de 2014, delimitandose un área de 100 cm2 como parámetro para ambas recolecciones. Se recolectaron las muestras a través de swabs estériles que fueron humedecidos y transportados en Carry y Blair. Los microorganismos fueron aislados, clasificados y después probados repecto a la resistencia antimicrobiana. Resultados el género Staphylococcus coagulasa negativo fue el más prevalente. Las pruebas de susceptibilidad a antimicrobianos mostraron algunos Staphylococci como resistentes a múltiples fármacos. Conclusión se señala la necesidad de ampliación del debate entre los profesionales de la salud, sobre cuestiones de seguridad hospitalaria, presentando la educación permanente como un posible camino de éxito en el control de las infecciones.

RESUMO Objetivo determinar o perfil microbiológico de bactérias isoladas e identificadas nos leitos e bombas infusoras na unidade de terapia intensiva de um hospital universitário do Estado do Rio de Janeiro. Método foram coletadas nove amostras de grades das camas dos pacientes e oito de teclados de bomba infusora de uma unidade de terapia intensiva, em outubro de 2014, delimitando-se uma área de 100cm2 como parâmetro para ambas as coletas. As amostras foram coletadas através de swabs estéreis que foram umedecidos e transportados em Carry & Blair. Os microrganismos foram isolados, classificados e depois testados em relação à resistência antimicrobiana. Resultados o gênero Staphylococcus coagulase negativa foi o mais prevalente. Os testes de suscetibilidade a antimicrobianos apontaram alguns destes Staphylococci como multirresistentes. Conclusão chama-se atenção para a necessidade de ampliação do debate multiprofissional sobre questões de segurança hospitalar, apresentando a educação permanente como um possível caminho de sucesso no controle das infecções.

Nosocomial infections are a relevant factor in complicating the recovery of patients interned for even minor causes. In attempt to determine their origin it is crucial to consider that their origin is of an endogenous nature. Looking for an acessible expression of intestinal colonization we analyzed fecal samples from 3 separate groups of hospital patients collected after different lenghts of time. For practical reasons one group was studied prospectively and two other groups (patients hospitalized for up to 7 days and patients hospitalized for more than 7 days) were compared to one another. We looked for the emergence of tellurite resistance among Enterobacteriaceae using a selective medium. MacConkey potassium tellurite (MCPT). The frequence of prospectively studied patients with tellurite resistant strains was significantly greater after 7 days of hospitalization. For the two other groups, patients with more than 7 days of hospitalization showed a significant increase of bacterial species and of strains with new antimicrobial resistance markers. High molecular weigth plasmids were detected in some of these strains. These data show that the MCPT medium is a useful tool for the investigation of bowel colonization in hospitalized patients by drug-resistant Enterobacteriaceae.