ABSTRACT Soils are the largest terrestrial carbon (C) reservoir, and most of this C is retained as soil organic matter (SOM). Due to its ability to capture, stabilize, and store C for extended periods, soils are considered important allies in decarbonizing the atmosphere. The term ‘C stabilization’ includes a series of mechanisms or processes by which soil C is protected within soils and its losses are reduced through microbial decomposition or leaching. Due to their relevance in the global C cycle, C stabilization mechanisms have received intensive attention from the scientific community. As new analytic technologies push the boundaries of what was previously possible to know, new paradigms emerge. This literature review summarizes the current knowledge of the main mechanisms that may promote SOM stabilization. Factors that govern accumulation of SOM are also addressed. We highlight the role of organo-mineral associations and spatial inaccessibility of SOM due to occlusion within soil aggregates to understand the relative contribution of these mechanisms in different soil conditions (e.g., soil texture, mineralogy, and land- use). In addition, the contribution of cutting-edge approaches and analytical techniques to advance the understanding of SOM protection is presented. Modern techniques to evaluate SOM on a micro, nano, and molecular scale can contribute to the mechanistic understanding of SOM stabilization and the study and adoption of management strategies that maintain and increase C stocks in soils.

ABSTRACT: The use of data mining is a promising alternative to predict soil respiration from correlated variables. Our objective was to build a model using variable selection and decision tree induction to predict different levels of soil respiration, taking into account physical, chemical and microbiological variables of soil as well as precipitation in renewal of sugarcane areas. The original dataset was composed of 19 variables (18 independent variables and one dependent (or response) variable). The variable-target refers to soil respiration as the target classification. Due to a large number of variables, a procedure for variable selection was conducted to remove those with low correlation with the variable-target. For that purpose, four approaches of variable selection were evaluated: no variable selection, correlation-based feature selection (CFS), chisquare method (χ2) and Wrapper. To classify soil respiration, we used the decision tree induction technique available in the Weka software package. Our results showed that data mining techniques allow the development of a model for soil respiration classification with accuracy of 81 %, resulting in a knowledge base composed of 27 rules for prediction of soil respiration. In particular, the wrapper method for variable selection identified a subset of only five variables out of 18 available in the original dataset, and they had the following order of influence in determining soil respiration: soil temperature > precipitation > macroporosity > soil moisture > potential acidity.

Mechanized sugarcane (Saccharum spp.) harvest without burning has been increasingly adopted in Brazil, increasing trash availability on the field. This study aims at showing the importance of using an integrated framework tool to assess technical and economic impacts of integral harvesting and baling trash recovery strategies and different recovery rates as well as its implications in the sugarcane production, transport and processing stages. Trash recovery using baling system presents higher costs per unit of mass of recovered trash in comparison to system in which trash is harvested and transported with sugarcane stalks (integral harvesting system). However, the integrated agricultural and industrial assessment showed that recovering trash using baling system presents better economic results (higher internal rate of return and lower ethanol production cost) than the integral harvesting system for trash recovery rates higher than 30 %. Varying trash recovery fraction, stalks productivity and mean transport distance for both integral harvesting and baling systems, sensitivity analyses showed that higher trash recovery fractions associated with higher stalks yields and long transport distances favors baling system, mainly due to the reduction of bulk load density for integral harvesting system under those conditions.

Strategies to mitigate climate change through the use of biofuels (such as ethanol) are associated not only to the increase in the amount of C stored in soils but also to the reduction of GHG emissions to the atmosphere.This report mainly aimed to propose appropriate methodologies for the determinations of soil organic carbon stocks and greenhouse gas fluxes in agricultural phase of the sugarcane production. Therefore, the text is a piece of contribution that may help to obtain data not only on soil carbon stocks but also on greenhouse gas emissions in order to provide an accurate life cycle assessment for the ethanol. Given that the greenhouse gas value is the primary measure of biofuel product quality, biorefiners that can show a higher offset of their product will have an advantage in the market place.

Due to new possibilities for using sugarcane (Saccharum spp.) trash for electricity generation, and the production of 2nd generation ethanol and others chemicals, the interest for its recovery has increased. However, the question of how much trash can be removed from sugarcane field still needs to be clarified. This study evaluated the amount of dry matter, nutrients content, structural compounds and efficiency of the enzymatic hydrolysis of the hydrothermal pretreated materials for tops and dry leaves in samples from sugarcane varieties. Tops and dry leaves present differences in nutrients content and moisture. Therefore, the amount of trash to be collected should not be simply based on percentages, but also should take into account the different fractions of the crop residues. For instance, around 80 % of N, P and K were derived from tops. Therein, the environmental indicators of the entire chain of sugarcane could be benefited because more nutrients would be recycled and less mineral fertilizers might be used for sugarcane production if tops are left on the field. Further, the tops have seven times more moisture than dry leaves and higher amounts of extractives (organic compounds of low molecular weight). Moreover, as the result of yield obtained in the pretreatment steps for dry leaves were superior to the tops and the glucose yields obtained in the enzymatic hydrolysis step were similar, it can be predicted that for second generation ethanol production, it is more viable to recover parts of the dry leaves fraction, leaving the tops on the field.

Sugarcane (Saccharum spp.) crops provide carbon (C) for soil through straw and root system decomposition. Recently, however, sugarcane producers are considering straw to be removed for electricity or second generation ethanol production. To elucidate the role of straw and root system on the carbon supply into the soil, the biomass inputs from sugarcane straw (tops and dry leaves) and from root system (rhizomes and roots) were quantified, and its contribution to provide C to the soil was estimated. Three trials were carried out in the State of Sao Paulo, Brazil, from 2006 to 2009. All sites were cultivated with the variety SP81 3250 under the green sugarcane harvest. Yearly, post-harvest sugarcane residues (tops, dry leaves, roots and rhizomes) were sampled; weighted and dried for the dry mass (DM) production to be estimated. On average, DM root system production was 4.6 Mg ha-1 year-1 (1.5 Mg C ha-1 year-1) and 11.5 Mg ha-1 year-1 (5.1 Mg C ha-1 year-1) of straw. In plant cane, 35 % of the total sugarcane DM was allocated into the root system, declining to 20 % in the third ratoon. The estimate of potential allocation of sugarcane residues to soil organic C was 1.1 t ha-1 year-1; out of which 33 % was from root system and 67 % from straw. The participation of root system should be higher if soil layer is evaluated, a deeper soil layer, if root exudates are accounted and if the period of higher production of roots is considered.

The maintenance of sugarcane (Saccharum spp.) straw on a soil surface increases the soil carbon (C) stocks, but at lower rates than expected. This fact is probably associated with the soil management adopted during sugarcane replanting. This study aimed to assess the impact on soil C stocks and the humification index of soil organic matter (SOM) of adopting no-tillage (NT) and conventional tillage (CT) for sugarcane replanting. A greater C content and stock was observed in the NT area, but only in the 0-5 cm soil layer (p < 0.05). Greater soil C stock (0-60 cm) was found in soil under NT, when compared to CT and the baseline. While C stock of 116 Mg ha-1 was found in the baseline area, in areas under CT and NT systems the values ranged from 120 to 127 Mg ha-1. Carbon retention rates of 0.67 and 1.63 Mg C ha-1 year-1 were obtained in areas under CT and NT, respectively. Laser-Induced Fluorescence Spectroscopy showed that CT makes the soil surface (0-20 cm) more homogeneous than the NT system due to the effect of soil disturbance, and that the SOM humification index (H LIF) is larger in CT compared to NT conditions. In contrast, NT had a gradient of increasing H LIF, showing that the entry of labile organic material such as straw is also responsible for the accumulation of C in this system. The maintenance of straw on the soil surface and the adoption of NT during sugarcane planting are strategies that can increase soil C sequestration in the Brazilian sugarcane sector.

Mudanças de uso da terra e práticas de manejo modificam a dinâmica do C e a fertilidade do solo. Este estudo avaliou as implicações dos sistemas de cultivo (NT e CT) nos estoques de C e de nutrientes e identificou inter-relações entre estes estoques e outros atributos da fertilidade do solo em Latossolo após a mudança do uso da terra no cerrado amazônico. Os estoques de C e de nutrientes (P, K, Ca e Mg) ajustados pela massa equivalente do solo sob cerrado (CE), foram maiores principalmente sob NT. Após a adoção do NT, exceto em 2NT, os estoques de C foram maiores em relação às demais áreas avaliadas. Correlações entre estoques de C e de nutrientes revelaram algumas correlações positivas com Ca e Mg nas áreas sob NT, devido ao uso continuo de calcário, à maior quantidade de resíduos culturais e ao não revolvimento do solo, associado à correlações positivas com CTC, saturação por bases e pH. A correlação positiva (r = 0,91, p < 0,05) entre estoques de C e CTC em CE indica a importante contribuição da MOS na CTC de solos tropicais, embora os sítios de troca estejam ocupados principalmente por H + Al. Estoques de P e K mostraram correlações positivas com estoques de C em CE (0,81 e 0,82; respectivamente), indicando a alta relação de P e K com a MOS em ecossistemas naturais. A alta variabilidade espacial associada à aplicação de fertilizantes (P e K) no sulco de plantio pode ter mascarado os resultados dos estoques. A principal fonte destes nutrientes para o solo foi o fertilizante aplicado e não a MOS.

Land use change and land management practices can modify soil carbon (C) dynamics and soil fertility. This study evaluated the effect of tillage systems (no-tillage - NT and conventional tillage - CT) on soil C and nutrient stocks in an Oxisol from an Amazonian cerrado following land use change. The study also identified relationships between these stocks and other soil attributes. Carbon, P, K, Ca and Mg stocks, adjusted to the equivalent soil mass in the cerrado (CE), were higher under NT. After adoption of all but one of the NT treatments, C stocks were higher than they were in the other areas we considered. Correlations between C and nutrient stocks showed positive correlations with Ca and Mg under NT due to continuous liming, higher crop residue inputs and lack of soil disturbance, associated with positive correlations with cation exchange capacity (CEC), base saturation and pH. The positive correlation (r = 0.91, p < 0.05) between C stocks and CEC in the CE indicates the important contribution of soil organic matter (SOM) to CEC in tropical soils, although the exchange sites are - under natural conditions - mainly occupied by H and Al. Phosphorus and K stocks showed positive correlations (0.81 and 0.82, respectively) with C stocks in the CE, indicating the direct relationship of P and K with SOM in natural ecosystems. The high spatial variability of P and K fertilizer application may be obscuring these soil nutrient stocks. In this study, the main source of P and K was fertilizer rather than SOM.

No sistema de plantio convencional, a prática anual de revolvimento do solo provoca alterações nas propriedades físicas, como diminuição da porosidade, modificação da estabilidade de agregados e destruição dos canais deixados pelo sistema radicular. No sistema de plantio direto, não há revolvimento do solo. Portanto, a aplicação de calcário, assim como de N, deve ser feita em superfície, em razão da impossibilidade de sua aplicação em profundidade. Objetivou-se com este trabalho estudar a movimentação de NO- e NH+ em colunas de solo indeformado, com e sem aplicação de calcário. Conduzido em casa-de-vegetação, utilizaram-se tubos de PVC com 0,2 m de diâmetro e 0,2 m de comprimento. As amostras de um Latossolo Vermelho Distrófico típico de textura muito argilosa, localizado no campus da UFLA, foram retiradas do horizonte A (0-0,2 m) inde-formadas. A irrigação e a análise do lixiviado foram feitas diariamente. No lixiviado, foram medidos o NO- e o NH+ . As análises de nitrato foram feitas por dois métodos diferentes, sendo um pela redução do nitrito na coluna de Cd-Cu e o outro por um eletrodo seletivo; as análises de NH+ foram feitas pelo método do Nessler. O delineamento experimental utilizado foi o delineamento inteiramente casualizado, com três repetições. Mesmo com doses realistas de adubos nitrogenados (200 kg N ha') e em Latossolos muito argilosos, com moderados a altos teores de óxidos de Fe e gibbsita, a lixiviação de NO- é uma realidade.

In the conventional tillage, the annual practice of revolving the soil leads to modifications in the soil physical properties as decrease of the porosity, modification of aggregate stability and destroction of the channels left by the root system. In the no-till system, there is no revolving of the soil. Therefore, liming as well as nitrogen fertilization applied must be made on the surface due to impossibility of this application in depth. The objective of this work was to study the movement of NO-and NH+ in undisturbed soil columns, with and without liming application. The present study was carried out in the greenhouse using PVC tubes of 0.2 m of diameter and 0.2 m of length using a very clayey typical dystroferric Red Latosol, located in the UFLA campus. The undisturbed soil samples were taken in the A horizon (0-0.2 m). The irrigation and the analyses of the leached water were made daily. In the leached water, it were measured NO- and NH+ . The NO- analysis were done by two different methods. The first one is based on the NO- reduction to NO- in a Cd-Cu column. The second method used a selective electrode. The NH+ were done by the Nessler method. The experimental design was the complete randomized, with three replications. Even with usual doses of N (200 kg ha-1) amendments in a very clayey Latosols, with moderate to high Fe and Al oxide content, NO- leaching was observed.