Abstract The present study aimed to screen genotypes for the presence/absence of lipoxygenases and to evaluate the phytochemical characteristics of lipoxygenase-free genotypes. Progenies derived from the cross between the cultivars IAC PL-2 (with lipoxygenases) and BRS 213 (lipoxygenase-free) generated 114 inbred lines in the F6/7 generation, evaluated by the qualitative colorimetric method, to verify the presence/absence of lipoxygenase isoenzymes L-1, L-2 and L-3. Eight triple-null (lipoxygenase-free) lines were confirmed by visible spectrophotometry. Progenitors and triple-null lines IAC 10-0003-1, IAC 10-0010-1, IAC 10-0010-2, IAC 10-0012, IAC 10-0043, IAC 10-0046, IAC 10-0107-1 and IAC 10-0115-2 were analyzed to assess their contents of protein, oil, phytic acid, tannins, total phenols and total flavonoids. Analyses showed significant differences in protein (from 38.80% to 41.89%) and oil (from 18.86% to 22.88%), consistent with the values observed in commercial soybean cultivars. The new triple-null lines, compared to the BRS 213 standard, showed lower contents of soluble condensed tannins; lower phytic acid in line IAC 10-0043 and greater amount of total phenols in line IAC 10-0115-2; whereas, the lines IAC 10-0010-1, IAC 10-0010-2, IAC 10-0043, IAC 10-0046 e IAC 10-0107-1 stood out with higher values of total flavonoids. The new lines will be potentially useful parents for genetic breeding and for a multiline strategy, aiming at greater phenotypic stability of lipoxygenase-free soybeans for human consumption. presenceabsence presence absence lipoxygenasefree free PL2 PL 2 PL- 21 (lipoxygenase-free 11 F67 F F6 7 F6/ generation method L1, L1 L 1, 1 L-1 L2 L- L3. L3 3. 3 L-3 triplenull triple null spectrophotometry 1000031, 1000031 10 0003 10-0003-1 1000101, 1000101 0010 10-0010-1 1000102, 1000102 2, 10-0010-2 100012, 100012 0012, 0012 10-0012 100043, 100043 0043, 0043 100046, 100046 0046, 0046 1001071 0107 10-0107- 1001152 0115 10-0115- tannins flavonoids 3880 38 80 38.80 41.89% 4189 41 89 1886 18 86 18.86 22.88%, 2288 22.88% , 22 88 22.88%) standard 10-004 whereas strategy consumption 100003 000 10-0003- 100010 001 10-0010- 10001 10-001 10004 004 100107 010 10-0107 100115 011 10-0115 388 8 38.8 41.89 418 4 188 18.8 228 22.88 10-00 10000 00 10-0003 10-0010 1000 10010 01 10-010 10011 10-011 38. 41.8 18. 22.8 10-0 0 10-000 100 1001 10-01 41. 22. 10-
Resumo O presente estudo teve como objetivo selecionar genótipos quanto à presença/ausência das lipoxigenases, além de avaliar as características fitoquímicas dos genótipos com ausência das lipoxigenases. Progênies oriundas do cruzamento entre as cultivares IAC PL-2 (com lipoxigenases) e BRS 213 (sem lipoxigenases) geraram 114 linhagens na geração F6/7, avaliadas pelo método qualitativo colorimétrico, para constatar a presença/ausência das isoenzimas lipoxigenases L-1, L-2 e L-3. Foram confirmadas por espectrofotometria na região do visível oito linhagens triplo-nulas (sem lipoxigenases). Os progenitores e as linhagens triplo-nulas IAC 10-0003-1, IAC 10-0010-1, IAC 10-0010-2, IAC 10-0012, IAC 10-0043, IAC 10-0046, IAC 10-0107-1 e IAC 10-0115-2 foram analisados para aferir seus teores de proteína, óleo, ácido fítico, taninos condensados solúveis, fenóis totais e flavonoides totais. As análises apontaram diferenças significativas nos teores de proteínas (38,80% a 41,89%) e de óleo (18,86% a 22,88%), coerentes com os valores observados em cultivares comercializados de soja. As novas linhagens triplo-nulas, comparativamente ao padrão BRS 213, apresentam menores teores de taninos condensados, menor presença de ácido fítico na linhagem IAC 10-0043 e maior presença de fenóis totais na linhagem IAC 10-0115-2, enquanto as linhagens IAC 10-0010-1, IAC 10-0010-2, IAC 10-0043, IAC 10-0046 e IAC 10-0107-1 se destacam com valores superiores de flavonoides totais. As novas linhagens serão parentais potencialmente úteis para melhoramento genético e para uma estratégia de multilinhas, visando à maior estabilidade fenotípica de soja sem lipoxigenases, para alimentação humana. presençaausência PL2 PL 2 PL- 21 11 F67 F F6 7 F6/7 colorimétrico L1, L1 L 1, 1 L-1 L2 L- L3. L3 3. 3 L-3 triplonulas triplo nulas . 1000031, 1000031 10 0003 10-0003-1 1000101, 1000101 0010 10-0010-1 1000102, 1000102 2, 10-0010-2 100012, 100012 0012, 0012 10-0012 100043, 100043 0043, 0043 100046, 100046 0046, 0046 1001071 0107 10-0107- 1001152 0115 10-0115- proteína solúveis 38,80% 3880 38 80 (38,80 41,89% 4189 41 89 18,86% 1886 18 86 (18,86 22,88%, 2288 22,88% , 22 88 22,88%) triplonulas, nulas, 10-004 1001152, multilinhas humana F6/ 100003 000 10-0003- 100010 001 10-0010- 10001 10-001 10004 004 100107 010 10-0107 100115 011 10-0115 38,80 388 8 (38,8 41,89 418 4 18,86 188 (18,8 228 22,88 10-00 10000 00 10-0003 10-0010 1000 10010 01 10-010 10011 10-011 38,8 (38, 41,8 18,8 (18, 22,8 10-0 0 10-000 100 1001 10-01 38, (38 41, 18, (18 22, 10- (3 (1 (