Abstract The purpose of this population-based, observational, and cross-sectional study was to evaluate alterations in the oral cells of a population of older people from a Brazilian rural area, using the micronucleus technique to investigate possible associated genotoxic factors. A questionnaire was applied and clinical examination and collection of oral mucosal cells were performed for all older people (≥ 60 years) from a town in southern Brazil. Demographic and socioeconomic variables, deleterious habits (drinking and tobacco use), presence of gastro-oesophageal reflux disease (GERD), and the use of proton pump inhibitors (PPIs) were considered the exposure variables, whereas metanuclear changes (MCs) and the prevalence of cell micronuclei (MN) were considered outcomes. Out of 489 older people, 447 were included in the study, among whom 50.8% were men with a mean age of 70.9 years and 83.9% had a monthly family income greater than US$ 500.00. GERD symptoms were present in 36.2% of the individuals, and 29.1% used PPIs daily, 53.3% consumed alcoholic beverages, and 46.7% used tobacco. The analysis of 1,000 oral mucosal cells per subject showed a MN frequency of 0–2 per individual, and MCs were detected with an average of 15 units per individual (median = 11 per individual). Poisson regression did not show statistical association between the exposure variables and the outcomes (presence of MN and MCs), except for the use of PPIs, which was a protective factor for the prevalence of MN [PR 0.6 (CI 0.3–0,9)]. Age, sex, family income, tobacco use and drinking, and GERD were not associated with the number of MN and MCs in oral mucosal cells of the investigated older people.

Abstract The aim of this prospective epidemiological study was to evaluate the occurrence of incidents involving the craniofacial region of soccer players during three official FIFA competitions. The craniofacial incidents were identified by video analysis of all 144 matches of two FIFA World Cups (2014/2018) and the 2017 FIFA Confederations Cup. Data collection included incident type, incident severity, site affected, incident cause and referee decision. The multivariate Poisson regression model was performed to analyze the associations between covariates. A total of 238 incidents were observed in the craniofacial region (1.6 incidents/match), representing a rate of 48.5 incidents per 1000 hours. At least 80.6% of the matches presented at least one incident, and, in more than 60%, the referee’s decision was no foul. According to severity, 26.8% of the incidents were classified as having mild or high severity. Incidents involving lacerations or fracture presented higher severity compared with hits (IRR 3.45[95%CI: 1.89–6.30]). Head-to-head impacts showed an incidence of severe incidents twice as high as those involving upper extremities (IRR 2.01[95%CI:1.07–3.76]). A high number of craniofacial incidents were observed in the last FIFA competitions. Head-to-head impacts and lacerations or fractures were associated with higher incident severity.