ABSTRACT Purpose To evaluate hemostasis of the ovarian arteriovenous complex (OAVC) in relation to surgical time, practicality and feasibility in three ovariohysterectomy (OH) techniques for queens. Methods The experiment was performed on 21 female cats aged between six months and seven years, randomly arranged into three groups in a completely randomized design. Group one was spayed using the conventional three-clamp technique, group two using the OAVC knotting technique, and group three using the ovarian pedicle rotation technique. The student’s t-test and Tukey’s test were used to compare the mean surgical times. Results The conventional technique, which uses thread wires, was more laborious and required longer execution time compared to the other two techniques. The OAVC knotting technique was the fastest and had the least blood loss. Conclusions The use of techniques that do not use synthetic materials for OAVC hemostasis was proven to be appropriate in castration projects, provided that the surgical team has sufficient training.