RESUMO: O fungo Microdochium albescens pode interferir na germinação de sementes e na emergência de plântulas de arroz, porém não há indicação técnica para seu controle via tratamento de semente. Este trabalho teve como objetivo avaliar a eficiência de fungicidas em tratamento de sementes de arroz pré-germinado e sequeiro no controle de M. albescens. Sementes das cultivares Epagri 109, SCS116 Satoru, SCS121 CL e SCS122 Miura foram tratadas com os seguintes fungicidas (g i.a./100 kg de sementes): fluazinam + tiofanato metílico (9,45 + 63), piraclostrobina + tiofanato metílico (5 + 45), carboxina + tiram (60 + 60), metalaxil-M + tiabendazol + fludioxonil (3 + 22,5 + 3,75), carbendazim + tiram (45 + 105), carbendazim (45). Sementes sem tratamento com fungicida constituíram a testemunha. As sementes tratadas foram semeadas em meio de cultura de batata-sacarose-ágar (BSA) e incubadas a 25 ± 2°C e fotoperíodo de 12 horas por 14 dias. Sementes tratadas também foram submetidas ao teste de germinação. Fluazinam + tiofanato metílico e metalaxil-M + tiabendazol + fludioxonil apresentaram maior controle de M. albescens, não diferindo estatisticamente entre si, nas cinco cultivares e nos dois sistemas de cultivo. Contudo, os fungicidas testados proporcionaram germinação das sementes superior a 90% nas quatro cultivares.

ABSTRACT: The fungus Microdochium albescens can interfere in the germination of seeds and in the death of rice seedlings; however, there is not technical indication for its control by seed treatment. This research evaluated the efficiency of fungicides in the treatment of pre-germinated and dryland rice seeds in the control of M. albescens. Seeds of the cultivars, Epagri 109, SCS116 Satoru, SCS121 CL and SCS122 Miura were treated with the following fungicides (g a.i./100 kg of seeds): fluazinam + thiophanate methyl (9.45 + 63), pyraclostrobin + thiophanate methyl (5 + 45), carboxin + thiram (60 + 60), metalaxyl-M + thiabendazole + fludioxonil (3 + 22.5 + 3.75), carbendazim + thiram (45 + 105), carbendazim (45). Seeds without fungicide treatment constituted the control. The treated seeds were sown in potato-sucrose-agar (PSA) culture medium and incubated at 25 ± 2 °C and photoperiod of 12 hours for 14 days. Treated seeds were also submitted to the germination test. Fluazinam + thiophanate methyl and metalaxyl-M + thiabendazole + fludioxonil showed greater control of M. albescens, not differing statistically between them, in the five cultivars and in the two cultivation systems. The tested fungicides provided seed germination above 90% in the four cultivars.

RESUMO O objetivo deste trabalho foi quantificar a viabilidade de Microdochium albescens em sementes de arroz irrigado armazenadas durante a entressafra e quantificar sua incidência nas estruturas de sementes. Foram coletadas mensalmente amostras de sementes de arroz irrigado dos cultivares SCS122 Miura e SCS121 CL, armazenadas durante a entressafra 2018/19. A análise de patologia e quantificação de viabilidade foram realizadas em laboratório. A determinação da localização do fungo nas sementes foi quantificada separando-se lema, pálea, endosperma e embrião, com subsequente desinfestação e semeadura em meio de cultura. O fungo M. albescens apresentou durante nove meses viabilidade de armazenamento de sementes de 77,8%, considerando-se a média dos lotes e cultivares. A incidência média na lema foi de 48%, pálea 43,7%, endosperma 24,7% e embrião 20,7%. O fungo M. albescens mantém a viabilidade em sementes de arroz na entressafra e o micélio é detectado com maior incidência nos tecidos externos da cariopse.

ABSTRACT The aims of this study were to quantify the viability of Microdochium albescens in irrigated rice seeds stored during the time between harvests and to quantify its incidence in the seed structures. Samples of irrigated rice seeds of the cultivars SCS122 Miura and SCS121 CL were monthly collected and stored during the time between harvests 2018/19. Pathology analysis and viability quantification were performed in the laboratory. The location of the fungus in the seeds was determined by separating lemma, palea, endosperm and embryo, with subsequent disinfestation and sowing in culture medium. The fungus M. albescens showed 77.8% seed storage viability for nine months, considering the average of lots and cultivars. Average incidence was 48% in the lemma, 43.7% in the palea, 24.7% in the endosperm and 20.7% in the embryo. The fungus M. albescens remains viable in rice seeds between harvests, while mycelium is detected at a higher incidence in the external tissues of caryopsis.

RESUMO Macrophomina phaseolina é um patógeno de solo com uma grande variedade de hospedeiros e de difícil controle por práticas culturais. O objetivo deste estudo foi quantificar a severidade da podridão das raízes (SPR) causada por M. phaseolina em cultivares de feijoeiro comum e seu impacto nos componentes de produção. Os experimentos foram conduzidos em casa de vegetação com as cultivares de feijão comum ANFC-9 Agro Norte, BRS Esteio, BRS Estilo, BRS Pérola, FTS Soberano, IPR Campos Gerais, IPR Tangará, IPR Tuiuiú, IPR Uirapurú e TAA Dama. Três isolados de M. phaseolina obtidos de plantas infectadas de feijão, soja e milho foram inoculados nas cultivares de feijão. No estádio fenológico R9 da maturidade da colheita de grãos foram avaliados o SPR e os componentes de produção de massa seca de raízes (MSR), número de legumes por planta (NLP), número de grãos por planta (NGP) e massa de grãos por planta (MGP). Todas as cultivares de feijão foram suscetíveis a M. phaseolina, apresentando redução no NLP, NGP e MGP. A SPR foi superior a 75%, independentemente da origem dos isolados. Na média das cultivares houve decréscimo significativo de 54,3% na MSR quando comparadas aos tratamentos controle. As cultivares IPR Tangará, ANFC-9 Agro Norte, BRS Esteio, BRS Pérola e ‘FTS Soberano’ obtiveram redução em pelo menos um dos componentes da produção enquanto a cultivar IPR Tangará em todos. Observouse decréscimo médio de NLP, NGP e MGP de 7,9%, 7,6% e 7,2%; 13,4%, 12,8% e 6,7%; e 14,2%, 12,9% e 10,1%, considerando os isolados obtidos de feijão, soja e milho, respectivamente.

ABSTRACT Macrophomina phaseolina is a soilborne pathogen with a wide range of hosts and its control through agricultural practices is difficult. The aim of this study was to quantify the severity of root rot (SRR) caused by M. phaseolina in common bean cultivars and its impact on yield components. The experiments were carried out under greenhouse conditions with the common bean cultivars ‘ANFC-9 Agro Norte’, ‘BRS Esteio’, ‘BRS Estilo’, ‘BRS Pérola’, ‘FTS Soberano’, ‘IPR Campos Gerais’, ‘IPR Tangará’, ‘IPR Tuiuiú’, ‘IPR Uirapurú’ and ‘TAA Dama’. Three isolates of M. phaseolina obtained from infected plants of common bean, soybeans and maize were inoculated in the common bean cultivars. In the phenological stage R9 of grain harvest maturity, the SRR and the yield components of root dry mass (RDM), number of pods per plant (NPP), number of grains per plant (NGP) and grain mass per plant (GMP) were assessed. All common bean cultivars were susceptible to M. phaseolina, showing a reduction in NPP, NGP and GMP. SPR was greater than 75%, regardless of the origin of isolates. Considering the average of cultivars, there was a significant decrease of 54.3% in RDM, compared to control treatments. The cultivars ‘IPR Tangará’, ‘ANFC-9 Agro Norte’, ‘BRS Esteio’, ‘BRS Pérola’ and ‘FTS Soberano’ had a decrease in at least one yield component, while a reduction occurred in all components for cultivar ‘IPR Tangará’. There was an average decrease in NPP, NGP and GMP of 7.9%, 7.6% and 7.2%; 13.4%, 12.8% and 6.7%; and 14.2%, 12.9% and 10.1%, considering the isolates obtained from beans, soybeans and maize, respectively.

RESUMO: O objetivo deste trabalho foi identificar e quantificar fungos infectando sementes de arroz irrigado, produzidas nas safras 2015/16, 2016/17 e 2017/18, no sistema pré-germinado na região do Alto Vale do Itajaí, estado de Santa Catarina. Foram analisados 479 lotes de oito cultivares, sendo as sementes desinfestadas e semeadas em meio de cultura de batata-sacarose-ágar com incubação por sete dias a 25ºC ± 2 ºC e fotoperíodo de 12 horas. O principal fungo detectado nas três safras foi Microdochium albescens com 54,9% de incidência média, seguido de Alternaria padwikii (7,7%) e Bipolaris oryzae (3,3%).

ABSTRACT: The aim of this research was to identify and quantify fungi infecting irrigated rice seeds produced in the 2015/16, 2016/17 and 2017/18 harvests in the pre-germinated system in the Alto Vale do Itajaí Region, Santa Catarina State, Brazil. A total of 479 lots of eight cultivars were analyzed. Seeds were disinfected and sown in potato-sucrose-agar culture medium with incubation for seven days at 25ºC ± 2ºC and photoperiod of 12 hours. The main fungus detected in the three crops season was Microdochium albescens with 54.9% of average incidence, followed by Alternaria padwikii (7.7%) and Bipolaris oryzae (3.3%).

ABSTRACT Stalk rots compromise the translocation of water and nutrients, affecting grain filling. This study aimed to quantify the incidence of stalk rots in corn hybrids depending on sowing time and nitrogen topdressing rate, and to assess any correlation with grain yield. The experiment was conducted under field conditions in Atalanta, Santa Catarina state, Brazil, using a split-split-plot randomized block design. The hybrids AG 9025 PRO3 (super-early) and 30F53 VYH (early) were sown in the preferential (September 20) and late (December 5) sowing seasons with four nitrogen rates (0, 150, 300, and 450 kg·ha–1). The population density was 75,000 plants·ha–1 andthe nitrogen topdressing was carried out at the V4, V8, and V12 stages. The fungus Colletotrichum graminicola predominated in both hybrids and its presence was negatively correlated with stalk diameter and yield. The hybrid AG9025 PRO3 was more susceptible to stalk rot (35.1%) than the hybrid 30F53 VYH (8.8%). A higher incidence of stalk rot was observed in the late sowing season (31.1%) than in the preferential sowing season (11.2%). A decrease in the incidence of stalk rot was observed in the hybrid AG9025 PRO3 as nitrogen rates increased, but this behavior was not clearly observed in the hybrid 30F53 VYH.

RESUMO: O fungo Fusarium graminearum foi um dos primeiros patógenos descritos na cultura do arroz, no entanto, no Brasil, não há descrição de sua ocorrência em panículas. O objetivo desse estudo foi descrever os sintomas ocasionados pela infecção de F. graminearum em grãos de arroz irrigado. O experimento foi conduzido, em casa de vegetação, duas vezes, com a cultivar de arroz irrigado SCS 121CL e o Híbrido INOV CL no estádio R4 (florescimento). Dois isolados de espécies do complexo Fusarium graminearium, 15A (F. graminearium - 15ADON) e FmNiv (F, meridionale - Nivalenol) foram inoculados em panículas por aspersão de macroconídios, com acompanhamento da evolução dos sintomas até a colheita. Não houve diferença entre os isolados. Pontos de coloração parda foram observados nas glumas três dias após inoculação, evoluindo, posteriormente, para lesões de coloração marrom-claro de tamanho e forma irregular. Houve escurecimento total da gluma com coloração arroxeada quando os grãos encontravam-se no estádio de enchimento de grão (R6). Na maturação de colheita a gluma dos grãos apresentava coloração marrom-escuro. Grãos severamente infectados mostraram-se chochos e quebradiços.

ABSTRACT: The fungus Fusarium graminearum was one of the first pathogens described as causing infections in rice; however, in Brazil, there is no description of its occurrence in panicles. The present study aimed to describe the symptoms caused by F. graminearum infection in irrigated rice grains. The experiment was conducted in a greenhouse in duplicate using the irrigated rice cultivar SCS 121CL and hybrid INOV CL at the R4 (flowering) stage. Two isolate of Fusarium graminearium species complex 15A (F. graminearium - 15-ADON) and FmNiv (F. meridionale - Nivalenol), was inoculated onto panicles by spraying with macroconidia and the development of symptoms was monitored until harvest. There was no difference in symptoms among isolates. Light brown spots were observed in the glumes three days after inoculation. These later evolved into brown lesions of irregular shape and size. The glume darkened to purple when the grains were in the filling stage (R6). On maturation, the glume showed dark brown coloration. Severely infected grains were shriveled and brittle.

ABSTRACT Euschistus heros is one the most important pest insect in soybean fields in Brazil. Therefore, site-specific management applied for controlling E. heros can result in economic and environmental benefits. However, sampling protocols for assessing spatialtemporal variability of this soybean pest and, then, for guiding a more sustainable pest management, were still unknown. Thus, the objective of this study was to compare the efficiency of sampling grid sizes for monitoring the spatial variability of E. heros infestation along the soybean reproductive stages. Nine sampling campaigns were carried out using the beating sheet method, from R2 to R7 soybean phenological stages. It was used a sampling grid of 10 × 10 m, totaling 338 points in an soybean field of 3.4 ha, located in Londrina, PR, Brazil, during the 2011/2012 growing season. Increased sampling grid sizes (i.e., 10 × 10 m, 10 × 20 m, 20 × 20 m, 20 × 30 m, 30 × 30 m, 30 × 40 m, and 40 × 40 m) were simulated by deleting points from the initial (reference) sampling grid. The data were analysed through statistical and geostatistical procedures. Thematic maps were built and compared using Pearson’s correlation and relative deviation coefficient (RDC). Spatial variability distribution pattern of E. heros infestation was dependent on sampling grid size and infestation pressure, in which smaller sampling grids and higher E. heros infestations enabled a more accurate monitoring. Based on study, an accurate protocol (r > 0.70 and RDC < 20%) for monitoring high infestation (i.e., > 2 individual per meter) of E. heros in soybean reproductive stages should include sampling grids smaller or equal to 20 × 20 m.

A aplicação localizada de nitrogênio, baseada no índice relativo de clorofila das folhas, pode proporcionar inúmeros benefícios econômicos e ambientais, no entanto, o conhecimento sobre a metodologia de amostragem ainda é incipiente. Nesse sentido, este estudo objetivou avaliar o uso de diferentes malhas amostrais na caracterização da variabilidade espacial do índice relativo de clorofila das folhas da cultura do trigo e na confecção de mapas temáticos para a aplicação localizada de nitrogênio. Para determinação do índice relativo de clorofila, utilizou-se clorofilômetro CFL 1030, em uma malha amostral regular de 10 m x 10 m, com 472 pontos amostrais. A partir da malha amostral inicial, pela técnica de eliminação de pontos, realizou-se a simulação da utilização das seguintes malhas: 10 m x 20 m; 20 m x 20 m; 20 m x 30 m; 30 m x 30 m; 30 m x 40 m; e 40 m x 40 m. O aumento da malha amostral promoveu redução na acurácia do diagnóstico do índice relativo de clorofila, nas folhas de trigo. À medida que se aumentou a malha amostral, os mapas tornaram-se mais generalistas e informações da variabilidade espacial do índice relativo de clorofila foram perdidas. Malhas amostrais menores ou iguais a 20 m x 20 m mostraram-se eficazes para a detecção da variabilidade espacial do índice relativo de clorofila em folhas de trigo e para a confecção de mapas temáticos de aplicação localizada de nitrogênio.

Site-specific nitrogen application, based on relative chlorophyll index from leaves, may provide many economic and environmental benefits, however, the knowledge on sampling methodologies is still incipient. Thus, this study aimed to evaluate the use of different sampling grids to characterize the spatial variability of relative chlorophyll index of leaves from wheat crop and elaborate thematic maps for site-specific nitrogen application. For determining the relative chlorophyll index, a CFL 1030 chlorophyll meter was used on a regular sampling grid of 10 m x 10 m with 472 sampling points. Based on the initial sampling grid, by using the point elimination method, the simulation was performed in the following sampling grids: 10 m x 20 m; 20 m x 20 m; 20 m x 30 m; 30 m x 30 m; 30 m x 40 m; and 40 m x 40 m. The increase of the sampling grid reduced the diagnostic accuracy of relative chlorophyll index in wheat leaves. As the sampling grid increased, the maps became more general and information on the spatial variability of the relative chlorophyll index were lost. Sampling grids smaller or equal to 20 m x 20 m were effective to detect the spatial variability of the relative chlorophyll index in wheat leaves and enable the elaboration of thematic maps for site-specific nitrogen application.

O objetivo do trabalho foi estudar a eficiência das malhas amostrais, utilizadas nas áreas manejadas com agricultura de precisão, para a caracterização da variabilidade espacial dos teores de fósforo (P) e potássio (K). O estudo foi conduzido em 30 áreas agrícolas, localizadas no Rio Grande do Sul, cujos solos foram classificados como Latossolos Vermelhos. As dimensões das malhas amostrais estudadas foram: 100x100m (10 áreas), 142x142m (10 áreas) e 173x173m (10 áreas). Foram analisados os teores de P e K na profundidade de 0,00-0,10m. Os dados foram submetidos à análise estatística descritiva e a análise geoestatística. De modo geral, as áreas estudadas apresentaram teores médios de P e K adequados ao desenvolvimento das culturas. Entretanto, observaram-se locais com baixos teores de P, justificando fertilizações em sítio-específico. Com base em parâmetros geoestatísticos, as dimensões de malhas amostrais utilizadas são eficientes em captar as diferentes escalas da variabilidade espacial de P e K, porém, recomenda-se o uso de malhas amostrais menores, que considerem a variabilidade espacial a curtas distâncias.

The objective of the research was to evaluate the efficiency of the sampling grids, used in areas managed with precision agriculture, for the characterization of spatial variability levels of phosphorus (P) and potassium (K). The study was conducted in 30 agricultural areas, located in Rio Grande do Sul state, presenting soils classified as Oxisols. The sizes of the sampling grids studied were: 100x100m (10 areas), 142x142m (10 areas) and 173x173m (10 areas).Were analyzed the levels of P and K, in depth of sampling from 0.00-0.10m. The data were subjected to descriptive statistical analysis and geostatistical analysis. In, general, the areas showed average levels of P and K suitable for crops development. However, were observed subareas with low levels of P, justifying fertilization on site-specific. Based on geostatistical parameters, sizes of the sampling grids used are efficient to capture the different scales of spatial variability of P and K, however, it is indicated smaller sampling grids to consider the spatial variability over short distances.