Resumo: O objetivo deste trabalho foi avaliar a atividade antibacteriana in vitro de venenos e toxinas purificadas de serpentes sobre a bactéria fitopatogênica Ralstonia solanacearum. As avaliações foram realizadas em 17 venenos brutos (13 de Bothrops, 3 de Crotalus e 1 de Lachesis) e sete toxinas (1 de Bothrops e 6 de Crotalus). A atividade antibacteriana foi avaliada em meio MB1 que continha os tratamentos solubilizados (1 μL mL-1). Utilizou-se o total de 100 μL de suspensão bacteriana (8,4 x 109 UFC mL-1). Após incubação a 28°C, avaliou-se o número de colônias bacterianas às 24, 48 e 72 horas após a inoculação. O gel SDS-PAGE a 15% foi usado para analisar o perfil proteico das amostras, tendo-se utilizado 5 μg de proteína no ensaio. Além disso, os valores de concentração inibitória mínima (CIM) e concentração letal (CL50) foram determinados pelo método Probit. Os venenos e as toxinas foram capazes de reduzir mais de 90% do crescimento de R. solanacearum. Esses resultados foram ou equivalentes aos do controle positivo cloranfenicol ou até melhores. Enquanto os valores de CIM variaram de 4,0 a 271,5 μg mL-1, a CL50 variou de 28,5 μg mL-1 a 4,38 mg mL-1. Dez venenos brutos (7 de Bothrops e 3 de Crotalus) e duas toxinas (giroxina e crotamina) são abordagens promissoras para o controle da bactéria fitopatogênica R. solanacearum.

Abstract: The objective of this work was to evaluate the in vitro antibacterial activity of snake venoms and purified toxins on the phytopathogenic bacterium Ralstonia solanacearum. The evaluations were performed with 17 crude venoms (13 from Bothrops, 3 from Crotalus, and 1 from Lachesis) and seven toxins (1 from Bothrops and 6 from Crotalus). Antibacterial activity was assessed in MB1 medium containing solubilized treatments (1 μL mL-1). A total of 100 μL bacterial suspension (8.4 x 109 CFU mL-1) was used. After incubation at 28°C, the number of bacterial colonies at 24, 48, and 72 hours after inoculation was evaluated. SDS-PAGE gel at 15% was used to analyze the protein patterns of the samples, using 5 μg protein of each sample in the assay. Furthermore, the minimum inhibitory concentration (MIC) and lethal concentration (LC50) values were determined by the Probit method. Venoms and toxins were able to reduce more than 90% of R. solanacearum growth. These results were either equivalent to those of the positive control chloramphenicol or even better. While MIC values ranged from 4.0 to 271.5 μg mL-1, LC50 ranged from 28.5 μg mL-1 to 4.38 mg mL-1. Ten crude venoms (7 from Bothrops and 3 from Crotalus) and two purified toxins (gyroxin and crotamine) are promising approaches to control the phytopathogenic bacterium R. solanacearum.

RESUMO Objetivo: Avaliar a dose de radiação recebida pela equipe cirúrgica em cirurgias de coluna, incluindo entre aqueles que não são considerados trabalhadores com exposição ocupacional. Método: Todas as cirurgias de coluna realizadas num mesmo departamento num período de 12 meses foram avaliadas quanto à exposição dos cirurgiões, enfermeiros/instrumentadores e auxiliares trabalhando na sala cirúrgica à radiação do fluoroscópio tipo “C-arm”. A radiação foi medida por 15 dosímetros de filme posicionados nas lapelas, luvas dos profissionais e também em pontos padronizados da sala. Os filmes foram analisados no laboratório de dosimetria em coleções por período. Resultados: Durante 12 meses, 81 cirurgias de coluna foram realizadas pela mesma equipe, e cada cirurgia durou entre 1 e 6 horas. A dose total de radiação variou de 0,16 mSv a 2,29 mSv dependendo do local de mensuração. O local com maior exposição foi o punho do cirurgião principal. Conclusão: Os resultados mostraram que nas cirurgias de coluna no nosso serviço, as doses de radiação foram baixas e dentro dos limites legais. Porém, o treinamento dos profissionais é essencial para a proteção contra a radiação dos profissionais de saúde e seus pacientes. Nível de evidência I/b, estudo de coorte exploratório.

ABSTRACT Objective: To evaluate the radiation dose received by staff in spine surgeries, including those who are not considered occupationally exposed workers. Methods: All spinal surgeries performed in the same department during a period of 12 months were evaluated with regard to the exposure of surgeons, scrub nurses, and auxiliary personnel working in the operating room to radiation from C-arm fluoroscopy. Radiation was measured by 15 film badge dosimeters placed on the professionals’ lapels, gloves, and room standardized sites. The films were analyzed in the dosimetry laboratory by collections per period. Results: During the 12 months, 81 spinal surgeries were performed by the same team, with surgical times ranging from 1 to 6 hours. The total radiation dose ranged from 0.16 mSv to 2.29 mSv depending on the dosimetry site. The most exposed site was the wrist of the main surgeon. Conclusion: The results showed that in the spinal surgeries in our setting, the radiation doses are low and within legal limits. Nevertheless, constant training of professionals is essential for radiation protection of medical staff and patients. Level of evidence I/b, exploratory cohort study.

OBJECTIVE: The aim of the present study was to evaluate the influence of anthropometric characteristics and gender on postural balance in adults. One hundred individuals were examined (50 males, 50 females; age range 20-40 years). METHODS: The following body composition measurements were collected (using bone densitometry measurements): fat percentage (% fat), tissue (g), fat (g), lean mass (g), bone mineral content (g), and bone mineral density (g/cm2). In addition, the following anthropometric measurements were collected: body mass (kg), height (cm), length of the trunk-cephalic region (cm), length of the lower limbs (cm) and length of the upper limbs (cm). The following indices were calculated: body mass index (kg/m²), waist-hip ratio and the support base (cm²). Also, a postural balance test was performed using posturography variables with open and closed eyes. RESULTS: The analysis revealed poor correlations between postural balance and the anthropometric variables. A multiple linear regression analysis demonstrated that the whole group (female and male) height explained 12% of the medial-lateral displacement, 10% of the speed of oscillation, and 11% of the displacement area. The length of the trunk-cephalic length explained 6% of the displacement in the anteroposterior direction. With eyes closed, the support base and height explained 18% of the medial displacement, and the lateral height explained 10% of the displacement speed and 5% of the scroll area. CONCLUSION: Measured using posturography, the postural balance was only slightly influenced by the anthropometric variables, both with open and closed eyes. Height was the anthropometric variable that most influenced postural balance, both in the whole group and separately for each gender. Postural balance was more influenced by anthropometric factors in males than females.

Este artigo analisa a moderna retomada do debate do cálculo econômico socialista. Mostramos como as modificações no cinturão protetor do programa de pesquisa neoclássico possibilitaram a discussão do problema dos incentivos sob informação assimétrica nas novas propostas de socialismo de mercado. Nessas propostas, o problema do conhecimento tal como desenvolvido por Hayek no debate original será interpretado como um problema de informação. Defendemos a tese de que, embora os modelos tenham sido criticados por economistas afiliados a escola da escolha pública, as novas propostas de socialismo de mercado e seus críticos sofrem dos mesmos problemas apontados por Hayek no debate original.

This article examines the modern revival of socialist economic calculation debate. We show how changes in the protective belt of neoclassical research programme enabled the discussion of the problem of incentives under asymmetric information on the new proposals for market socialism. Under these proposals, the problem of knowledge as developed by Hayek in original debate will be interpreted as an information problem. We contend that, although the models were criticized by Public Choice economists, the new proposals and their critics both suffer from the same problems pointed out by Hayek in the original debate.

O presente estudo relata novos achados de carrapatos provenientes do Município de Vilhena, Sudeste do Estado de Rondônia, na região Norte do Brasil. Carrapatos colhidos de uma capivara, Hydrochoerus hydrochaeris (Linnaeus), foram identificados como Amblyomma romitii Tonelli-Rondelli (1 fêmea) e Amblyomma sp. (1 larva). Carrapatos colhidos de uma águia harpia, Harpia harpyja (Linnaeus), foram identificados como Amblyomma cajennense (Fabricius) (16 ninfas) e Haemaphysalis juxtakochi Cooley (1 ninfa). Carrapatos colhidos de um jabuti, Chelonoidis denticulada (Linnaeus), foram identificados como Amblyomma rotundatum Koch (10 fêmeas, 2 ninfas) e Amblyomma sp. (2 larvas). O presente achado de A. romitii é o primeiro no Estado de Rondônia, representando o achado mais meridional desta espécie de carrapato, indicando que sua distribuição geográfica é mais ampla do que se supunha. Embora A. cajennense e H. juxtakochi têm sido relatados parasitando várias espécies de aves, o presente relato em harpia é o primeiro registro de carrapatos nesta espécie de hospedeiro. A. rotundatum ocorre amplamente no Estado de Rondônia e tem sido previamente relatado parasitando jabutis. Estes registros ampliam a fauna de carrapatos de Rondônia para 26 espécies.

In the present study, we provide new tick records from Vilhena Municipality, in the Southeast of the State of Rondônia, Northern Brazil. Ticks collected from a capybara, Hydrochoerus hydrochaeris (Linnaeus), were identified as Amblyomma romitii Tonelli-Rondelli (1 female), and Amblyomma sp. (1 larva). Ticks collected from a harpy eagle, Harpia harpyja (Linnaeus), were identified as Amblyomma cajennense (Fabricius) (16 nymphs) and Haemaphysalis juxtakochi Cooley (1 nymph). Ticks collected from a yellow-footed tortoise, Chelonoidis denticulada (Linnaeus), were identified as Amblyomma rotundatum Koch (10 females, 2 nymphs), and Amblyomma sp. (2 larvae). The present record of A. romitii is the first in the State of Rondônia, and represents the southernmost record for this tick species, indicating that its distribution area is much larger than currently recognized. Although both A. cajennense and H. juxtakochi have been reported parasitizing various bird species, we provide the first tick records on a harpy eagle. A. rotundatum is widespread in the State of Rondônia, and has been previously reported on the yellow-footed tortoise. The present records increase the tick fauna of Rondônia to 26 species.