ABSTRACT An outbreak of bloodstream infection (BSI) caused by members of the Burkholderia cepacia complex (Bcc) took place from March 2012 until April 2014 involving thirteen patients. Aim. To describe an outbreak investigation of BSI Bcc and showing how genetic sequencing tools contributed to confirm the hypothesis of extrinsic contamination proposed by an observational study. Methods. The Infection Control Department revised and reinforced good practices of infusion therapy and catheter care, visits to affected wards, a case control study, and environmental screening based on the case-control findings. Results. Data from the case-control study found an association of cases with central venous catheter (OR 1.36; CI 1.15-1.67) and intravenous cisatracurium use (OR 10.75; CI 1.67-68.89). Visits to the operatory block revealed problems related to the cold chain used for the preservation of thermolabile cisatracurium. We could not retrieve Bcc from environmental samples using classic microbiology. New samples from the same surfaces were obtained for genetic sequencing. Bcc was identified in the cooler box, refrigerator and reusable ice packages. Conclusion. Environmental screening using genetic sequencing proved to be a useful tool for confirming our hypothesis of extrinsic contamination raised by the case-control study.

The present work reports an initial process of nursing assistance systematization developed in a Pediatric Unit of a medium size hospital in São Carlos - SP, considering the actual human resources and the type of approach adopted by the institution, on the assistance given to the hospitalized child. A Routine Manual was elaborated based on the literature and with the staff participation, and after that, a training was realized with these employees, starting from routines previously sketched, using groupal dynamics that looked forward to rescue the knowledge that they already had about the themes. The work considered the employees opinion about their participation, in the systematization process.

O presente trabalho relata um processo inicial de sistematização da assistência de enfermagem desenvolvido em unidade pediátrica de um hospital de médio porte, no interior do estado de São Paulo, considerando os recursos humanos existentes e o tipo de abordagem adotada pela instituição na prestação da assistência à criança hospitalizada. Elaborou-se, com base na literatura e com a participação dos funcionários, um manual de rotinas e em seguida, realizou-se um treinamento desses funcionários, através de uma dinâmica grupal que procurou resgatar os conhecimentos que eles já possuíam sobre os ternas. O trabalho considerou a opinião dos funcionários sobre sua participação no processo de sistematização e treinamento.

O presente trabalho relata um processo inicial de sistematização da assistência de enfermagem desenvolvido em unidade pediátrica de um hospital de médio porte, no interior do estado de São Paulo, considerando os recursos humanos existentes e o tipo de abordagem adotada pela instituição na prestação da assistência à criança hospitalizada. Elaborou-se, com base na literatura e com a participação dos funcionários, um manual de rotinas e em seguida, realizou-se um treinamento desses funcionários, através de uma dinâmica grupal que procurou resgatar os conhecimentos que eles já possuíam sobre os ternas. O trabalho considerou a opinião dos funcionários sobre sua participação no processo de sistematização e treinamento.

The present work reports an initial process of nursing assistance systematization developed in a Pediatric Unit of a medium size hospital in São Carlos - SP, considering the actual human resources and the type of approach adopted by the institution, on the assistance given to the hospitalized child. A Routine Manual was elaborated based on the literature and with the staff participation, and after that, a training was realized with these employees, starting from routines previously sketched, using groupal dynamics that looked forward to rescue the knowledge that they already had about the themes. The work considered the employees opinion about their participation, in the systematization process.