Abstract Fusariosis (Fusarium guttiforme) is the most destructive disease of the pineapple crop in Brazil. The aim of this study was to evaluate the reaction to an isolate in pineapple genotypes selected for crownless fruit weight (CFW) and total soluble solids (TSS). A total of 1,125 genotypes ('BRS Imperial'×'Pérola') were evaluated. Selection for greatest CFW and TSS was performed during the sexual cycle. The isolate was inoculated on slip-type plantlets using the immersion technique. Plantlets were evaluated for disease intensity in the stem, attributing scores from 0 to 5. Eighteen genotypes were selected with values from 1.514 to 3.307 g (CFW) and from 14.6 to 19.4 °Brix (TSS). Six genotypes reached the highest level of survival against the IT-01 isolate. Selected genotypes have agronomic traits superior to those of their parents, which qualify them to be cycled back into the plant breeding program of the Instituto Agronômico de Pernambuco - IPA.

Isolados de Colletotrichum gloeosporioides (ISO-1, ISO-2, ISO-3, ISO-4, ISO-5 e ISO-6), agente causal da antracnose em frutos de mangueira, foram caracterizados por meio de padrões eletroforéticos de proteínas totais e esterase, em gel de poliacrilamida e produção de enzimas extracelulares, em substratos sólidos específicos. A análise eletroforética mostrou variação no número, intensidade de coloração e posição das bandas no gel, dentro dos dois sistemas estudados. Verificou-se polimorfismo em relação a esterase, mostrando maior diferença entre os isolados, enquanto que nas proteínas totais, observou-se comportamento aparentemente uniforme. Quanto à produção de enzimas extracelulares: amilase, lípase e protease, todos os isolados, embora variando em comportamento, apresentaram atividade para essas enzimas, sendo aparentemente mais acentuada à atividade proteolítica.

Isolates of Colletotrichum gloeosporioides (ISO-1, ISO-2, ISO-3, ISO-4, ISO-5 and ISO-6), the causal agent of anthracnose disease on mango fruits, were characterized by electrophoretic patterns of total proteins and esterase in polyacrylamida gel, and also, by production of extracellular enzymes on specific solid substrate. The electrophoretic analysis showed variation in number, intensity of coloration and position of the bands in the gel at each studied system tested. In contrast to the monomorphic behavior to total proteins, high esterase polymorfism was observed indicating difference among isolates. All isolates showed the activity of extracellular enzymes such as amylase, lipase, and protease with some variation among them. The proteolitic activity seemed to be more accentuated than the two other enzymes studied.