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RESUMO Objetivo analisar a prevalência de Transtorno de Estresse Pós-Traumático e os fatores associados ao desenvolvimento do Transtorno de Estresse Pós-Traumático em profissionais de enfermagem durante a pandemia da COVID-19. Método estudo observacional, analítico e transversal, realizado com 309 profissionais de enfermagem, utilizando questionário para avaliar dados sociodemográficos e informações sobre o trabalho, além da Escala do Impacto do Evento - Revisada (IES-R), que visa coletar informações relacionadas à sintomatologia do Transtorno de Estresse Pós-Traumático. Resultados o estudo contou com 176 enfermeiros e 133 técnicos de enfermagem, sendo 83,82% do sexo feminino e 56,96% de enfermeiros. Quanto ao trabalho, 55,66% possuíam carga horária de até 40 horas semanais, 47,90% tinham mais de um vínculo, 89,32% atuaram na linha de frente da pandemia e 60,19% apontaram aumento da carga de trabalho. Não obstante, 64,40% apresentaram sintomas ou foram diagnosticados com COVID-19 e 43,37% apontaram prejuízo emocional. Utilizando as classificações do escore geral da Escala do Impacto do Evento - Revisada, 29, 53,40% atingiram pontuação maior ou igual a 33, ponto de corte para o provável diagnóstico de Transtorno do Estresse Pós-Traumático. Conclusão foi evidenciado que mais da metade da amostra do estudo apresentou na escala Escala do Impacto do Evento - Revisada alto risco de desenvolver Transtorno do Estresse Pós-Traumático. Como fatores associados ao desenvolvimento do Transtorno de Estresse Pós-Traumático em profissionais de enfermagem durante a pandemia da COVID-19, estão o uso de medicamentos psicotrópicos, idade até 35 anos, mudança financeira e emocional.

ABSTRACT Objective to analyze the prevalence of Post-Traumatic Stress Disorder and factors associated with its development in Nursing professionals during the COVID-19 pandemic. Method an observational, analytical and cross-sectional study conducted with 309 Nursing professionals, using a questionnaire to assess sociodemographic data and work-related information, in addition to the Impact Event Scale - Revised, which aims at collecting diverse information related to Post-Traumatic Stress Disorder symptoms. Results the study participants were 176 nurses and 133 nursing technicians: 83.82% female and 56.96% male nurses. As for the hour load, 55.66% asserted working up to 40 weekly hours 47.90% had more than one employment contract, 89.32% were active in the front line against the pandemic, and 60.19% reported an increase in workload. However, 64.40% presented symptoms or were diagnosed with COVID-19 and 43.37% indicated emotional impairment. Using the classifications of the overall Impact Event Scale - Revised score, 29 (53.40%) obtained scores of at least 33, the cutoff point for likely diagnosis of Post-Traumatic Stress Disorder. Conclusion it was evidenced that more than half of the study sample presented a high risk of developing Post-Traumatic Stress Disorder in the Impact Event Scale - Revised scale. Factors associated with the development of Post-Traumatic Stress Disorders in Nursing professionals during the COVID-19 pandemic include use of psychotropic drugs, age up to 35 years old, and occurrence of physical and emotional changes.

RESUMEN Objetivo analizar la prevalencia del Trastorno de Estrés Post-Traumático y los factores asociados al desarrollo de dicho trastorno en profesionales de Enfermería durante la pandemia de COVID-19. Método estudio observacional, analítico y transversal, realizado con 309 profesionales de Enfermería por medio de un cuestionario para evaluar datos sociodemográficos y diversa información sobre el trabajo, además de la Escala de Impacto del Evento - Revisada, que tiene como objetivo recolectar diversa información relacionada con la sintomatología del Trastorno de Estrés Post-Traumático. Resultados el estudio contó con la participación de 176 enfermeros y 133 técnicos de Enfermería: 83,82% del sexo femenino y 56,96% de enfermeros. En cuanto al trabajo, el 55,66% poseían una carga horaria de hasta 40 horas semanales, el 47,90% tenían más de un vínculo laboral, el 89,32% trabajaba en la primera línea de lucha contra la pandemia y el 60,19% señaló un aumento en la carga de trabajo. No obstante, el 64,40% presentó síntomas o fueron diagnosticados con COVID-19 y el 43,37% indicó perjuicios emocionales. Utilizando las clasificaciones de la puntuación general de la escala Escala de Impacto del Evento - Revisada, 29 (53,40%) obtuvieron un puntaje de al menos 33, punto de corte para el probable diagnóstico de Trastorno de Estrés Post-Traumático. Conclusión se hizo evidente que, en la escala Escala de Impacto del Evento - Revisada, más de la mitad de la muestra del estudio presentó alto riesgo de desarrollar el Trastorno de Estrés Post-Traumático. Como factores asociados al desarrollo del Trastorno de Estrés Post-Traumático en profesionales de Enfermería durante la pandemia de COVID-19 figuran los siguientes: uso de medicamentos psicotrópicos, edad de hasta 35 años, y cambios en estado financiero y emocional.

https://doi.org/10.1590/1980-265x-tce-2022-0139en

Heritability and combined parental information to define the number of crosses in circulant diallels
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Inocente, Gabriela

; Garbuglio, Deoclécio Domingos

; Araújo, Pedro Mario de

; Ruas, Paulo Maurício

Crop Breeding and Applied Biotechnology

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2021, Volume 21 Nº 2 elocation e37472125

Resumo: En | Texto: En | PDF: En

Abstract: In many agricultural crops, the number of parents used in cross breeding make it difficult to obtain all possible hybrids in diallels. Therefore, using maize as a model plant and based on traits with high and low heritabilities, the aim of the present study was to quantify the correlation estimates of the general combining ability (GCA) and specific combining ability (SCA) between a complete diallel and a circulant diallel, with and without the inclusion of parents. For the high heritability trait, the GCA estimates can be obtained with low s values, whereas for the SCA estimates, s values close to half the number of parents should be used. For the low heritability trait, information from parents must be used to obtain the SCA estimates. For the GCA estimates, considering the stabilization of r above 0.70 for s=7, s values greater than half the number of parents must be used in the circulant models.

https://doi.org/10.1590/1984-70332021v21n2c34 22 downloads

Validation of the criteria matrix about the generalist’s education in Dentistry Undergraduate Courses
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NORO, Luiz Roberto Augusto

; OLIVEIRA, Angelo Giuseppe Roncalli da Costa

; GURGEL, Bruno César de Vasconcelos

; ARAÚJO, Pedro Henrique de

Brazilian Oral Research

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2021, Volume 35 elocation e089

Resumo: En | Texto: En | PDF: En

Abstract The generalist training planned by Brazilian National Curriculum Guidelines for Dentistry undergraduate courses should provide actions for the development of health promotion in the field of public health, as well as disease prevention, diagnosis, planning, and dental treatment in the clinical field. Considering this complexity, the aim of the present study was to validate dimensions and sub-dimensions in dentistry training, allowing the identification of the main necessary competences and clinical skills for the generalist practice. For this, an evaluation study was carried out through the Delph’s Method with the participation of 19 professors of Dentistry courses in Brazil working in the clinical area, considered “experts” in this theme. It was possible to identify clinical competences and skills in four dimensions and respective sub-dimensions: Transversal skills (biosafety, patient-team communication, humanization, ethical principles, dental documentation); Diagnosis and planning (diagnosis of dental caries, pulp and periapical changes, and periodontal diseases, clinical and imaging exam, and single treatment planning); Preventive activities (oral hygiene guidance and oral cancer prevention); and Dental care (anesthesia, basic oral surgery, drug prescription, subgingival scaling and root planing, dental restoration, minimally and non-invasive restoration treatment, and dental urgencies). The developed model represents a differentiated alternative for the construction of an innovative curriculum in Dentistry, aimed at the training of general practitioners to provide humanized, highly effective, and resolving care. The importance of clinical tutoring by professors on the essential clinical skills and abilities is highlighted in this study.

https://doi.org/10.1590/1807-3107bor-2021.vol35.0089 5 downloads

Two validity evidences of the ESQUADA and Brazilians’ dietary quality levels
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Santos, Thanise Sabrina Souza

; Araújo, Pedro Henrique de Moura

; Andrade, Dalton Francisco de

; Louzada, Maria Laura da Costa

; Assis, Maria Alice Altenburg de

; Slater, Betzabeth

Revista de Saúde Pública

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2021, Volume 55 elocation 39

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO OBJETIVO Avaliar duas evidências de validade da escala de qualidade da dieta (ESQUADA) para seleção dos itens com melhor discriminação da qualidade da dieta dos brasileiros e propor uma descrição em níveis de escore. MÉTODOS Adolescentes e adultos brasileiros e residentes no país (n = 2.059), responderam a um questionário on-line com 52 itens, compartilhado em redes sociais e listas de correio eletrônico entre março e abril de 2018. Foram aplicados testes estatísticos para análise de evidências de validade e confiabilidade do instrumento. A análise fatorial foi aplicada para estudo da dimensionalidade dos itens do questionário. A teoria de resposta ao item foi aplicada para identificar a discriminação e localização dos itens no continuum , construir a escala e avaliar o comportamento diferencial dos itens quanto ao sexo e idade. RESULTADOS Dentre os 52 itens do questionário, 25 apresentaram maior precisão de medida, com ajuste e confiabilidade adequados. O item sobre o costume de comer alimentos ultraprocessados em casa apresentou a melhor discriminação da qualidade da dieta. Nenhum item apresentou comportamento diferencial quanto a sexo e idade. Na construção da ESQUADA foram identificados cinco níveis de qualidade da dieta: “muito ruim”, “ruim”, “boa”, “muito boa” e “excelente”. Observou-se que enquanto cereais matinais e/ou barrinhas de cereais são consumidos com maior frequência por indivíduos com qualidade da dieta “muito ruim”; castanhas e/ou nozes são consumidos mais frequentemente por aqueles indivíduos com qualidade da dieta “excelente”. CONCLUSÕES A ESQUADA é composta por 25 itens precisos e sem comportamento diferencial para avaliar a qualidade da dieta dos brasileiros. A construção da ESQUADA possibilitou reconhecer consumo e práticas alimentares característicos de cada nível de qualidade da dieta.

ABSTRACT OBJECTIVE Assess two validity evidences of the diet quality scale (ESQUADA) for the selection of items with better discrimination of the Brazilians’ diet quality and propose a description in score levels. METHODS Brazilian adolescents and adults residing in the country (n = 2,059) answered an online questionnaire with 52 items, shared on social networks and email lists between March and April 2018. Statistical tests were applied to analyze the validity and reliability of the instrument’s evidence. Factor analysis was applied to study the dimensionality of the questionnaire items. Item response theory was applied to identify the discrimination and location of items on the continuum, construct the scale and assess the differential item functioning in terms of sex and age. RESULTS Among the 52 items of the questionnaire, 25 had greater measurement accuracy, with adequate adjustment and reliability. The item on the habit of eating ultra-processed foods at home showed the best discrimination of diet quality. No item showed differential functioning regarding sex and age. In the construction of the ESQUADA, five diet quality levels were identified: very poor, poor, good, very good and excellent. It was observed that while breakfast cereals and/or cereal bars are more frequently consumed by individuals with “very poor” diet quality; nuts and/or walnuts are most often consumed by those individuals with “excellent” diet quality. CONCLUSION The ESQUADA consists of 25 precise items with no differential functioning to assess the quality of Brazilians’ diet. The construction of the ESQUADA made it possible to recognize food consumption and dietary practices characteristic of each level of diet quality.

https://doi.org/10.11606/s1518-8787.2021055002397

Cationic polymerization of styrene using iron-containing ionic liquid catalysts in an aqueous dispersed medium
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Dutra, Gabriel Victor Simões

; Silvério Neto, Weslany

; Araújo, Pedro Henrique Hermes de

; Sayer, Claudia

; Silveira Neto, Brenno Amaro da

; Machado, Fabricio

Polímeros

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2021, Volume 31 Nº 1 elocation e2021005

Resumo: En | Texto: En | PDF: En

Abstract This work aims to study the cationic miniemulsion polymerization of styrene catalyzed by iron-containing imidazolium-based ionic liquids. The polystyrenes had very high number-average molar mass around 1300 kg mol-1 at 85 °C, molar-mass dispersity close to 2.0 and glass transition temperature higher than 102 °C with average particle diameter that remained practically unchanged during the reaction, indicating that the monomer droplets correspond to the polymerization locus. First-order kinetics up to a limit conversion, along with the increase in molar mass as the temperature decreases, styrene polymerization at low temperatures and catalyst inability to polymerize monomers that react exclusively via free radical and/or anionic polymerization, indicate the cationic nature of polymerization. 1H-NMR and 13C-NMR spectra suggested the formation of polystyrene, allowing for tacticity distribution quantification: 10% isotactic, 20% atactic and 70% syndiotactic configurations. TEM micrographs confirmed the formation of spherical polymer nanoparticles and the presence of catalysts in the polymer matrix.

https://doi.org/10.1590/0104-1428.04620 221 downloads

Two validity evidences of the ESQUADA and Brazilians’ dietary quality levels
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Santos, Thanise Sabrina Souza

; Araújo, Pedro Henrique de Moura

; Andrade, Dalton Francisco de

; Louzada, Maria Laura da Costa

; Assis, Maria Alice Altenburg de

; Slater, Betzabeth

Revista de Saúde Pública

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2021, Volume 55 elocation 39

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

https://doi.org/10.11606/s1518-8787.2021055002397

Análise do perfil epidemiológico dos pacientes submetidos a transplante de córnea no Espírito Santo
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Pedro, Sara Araujo

; Araújo, Pedro Henrique de Andrade

; Bicalho, Júlia Antunes Rizzo

; Alves, Solayne Silva

; Magalhães, Bárbara Ahnert Blanco de Moura

; Lemos, Lucas Durão de

; Silva, Mayara da

; Machado, Maria dos Santos

; Kataoka, Flávio Takemi

Revista Brasileira de Oftalmologia

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Dez 2020, Volume 79 Nº 6 Páginas 370 - 373

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Objetivo: Descrever o perfil epidemiológico dos pacientes submetidos a transplante de córnea no estado do Espírito Santo. Métodos: Estudo transversal e descritivo realizado entre o período de janeiro de 2017 a janeiro de 2018, com o total de 1310 cadastrados na Central de Notificação, Captação e Distribuição de Órgãos do Espírito Santo (CNCDO/ES) por meio do Sistema Nacional de Transplantes (SNT) e que foram submetidos a transplante de córnea. Os dados foram apresentados em estatística descritiva como frequências e mediana e a análise dos dados foi realizada com o software SPSS versão 23. Resultados: em relação à frequência, obteve-se 54,3% do gênero feminino, 57,33% (751) provenientes da Região Metropolitana de Vitória. Os pacientes do Espírito Santo correspondem a 86% da frequência na fila, seguidos por Rio de Janeiro, Minas Gerais e Bahia, que juntos correspondem a 6,3%. O principal diagnóstico na indicação de transplante foi a Ceratopatia Bolhosa (25,9%), seguido por Ceratocone (16,5%) e Ceratite Intersticial (15,5%). A média de tempo de espera foi de 195 dias, sendo no máximo 1345 dias. Verificou-se que 1310 pacientes foram transplantados de córnea, a faixa etária dos transplantados é de 57 anos, sendo a maioria destes da Região Metropolitana de Vitória. Conclusão: Os diagnósticos mais prevalentes nos pacientes transplantados no Espírito Santo foram Ceratopatia Bolhosa e Ceratocone, em pacientes majoritariamente do sexo feminino, 57 anos em média, da região metropolitana da grande Vitória e provenientes do próprio estado. O estudo permite traçar estratégias de prevenção, cuidado e ações sociais de conscientização de doação de córnea.

Abstract Objective: To describe the epidemiological profile of patients submitted to corneal transplantation in state of Espirito Santo, Brazil. Methods: Transversal and descriptive study, with 1301 patients, in the period of January 2017 until to January 2018, the patients were registered in the Central Notification, Captation and Distribution of Organs of Espirito Santo (CNCDO/ES) through the National Transplant System (SNT) and submitted to corneal transplantation. The data were shown as descriptive statistics (frequencies and medians) and analysis of the data was performed using SPSS software, 23 version. Results: In relation to frequency, it was obtained 54.5% female, 57.33% from the metropolitan area of Vitória. Eighty-six percent were patients from Espirito Santo and the others 6.3% were from Rio de Janeiro, Minas Gerais and Bahia. The main indications for corneal transplantation were Bullous Keratopathy (25.9%), Keratoconus (16.5%) and interstitial keratitis (15.5%). The average of waiting time was 195 days and the longest was 1345 days. It was found that 1301 patients underwent corneal transplantation, they are in the 57-year age group, and the biggest part of them are from the metropolitan area of Vitória. Conclusion: The most prevalent diagnoses in patients submitted to corneal transplantation in state of Espirito Santo were Bullous Keratopathy and Keratoconus, in patients mostly female, 57 years old on average, from the metropolitan region of greater Vitória and from the state itself. The study allows to outline prevention strategies, care and social actions to raise awareness of corneal donation.

https://doi.org/10.5935/0034-7280.20200081 707 downloads

Neurotology: definitions and evidence-based therapies - Results of the I Brazilian Forum of Neurotology
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Salmito, Márcio Cavalcante

; Maia, Francisco Carlos Zuma e

; Gretes, Mário Edvin

; Venosa, Alessandra

; Ganança, Fernando Freitas

; Ganança, Maurício Malavasi

; Mezzalira, Raquel

; Bittar, Roseli Saraiva Moreira

; Gasperin, Alexandre Camilotti

; Pires, Anna Paula Batista de Ávila

; Ramos, Bernardo Faria

; Bertoldo, César

; Ferreira Jr., Cícero

; Real, Danilo

; Guimarães, Humberto Afonso

; Oiticica, Jeanne

; Lavinsky, Joel

; Lopes, Karen Carvalho

; Duarte, Juliana Antoniolli

; Morganti, Lígia Oliveira Gonçalves

; Santos, Lisandra Megumi Arima dos

; Joffily, Lúcia

; Lavinsky, Luíz

; Santos, Mônica Alcantara de Oliveira

; Mano, Patrícia Mauro

; Araújo, Pedro Ivo Machado Pires de

; Mangabeira Albernaz, Pedro Luís

; Cal, Renato

; Dorigueto, Ricardo Schaffeln

; Guimarães, Rita de Cássia Cassou

; Carvalho, Rogério Castro Borges de

Brazilian Journal of Otorhinolaryngology

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Abr 2020, Volume 86 Nº 2 Páginas 139 - 148

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Introdução: A otoneurologia é uma área de conhecimento que tem se expandido muito rapidamente. O estudo do sistema vestibular tem avançado tanto que mesmo definições básicas, como o significado dos sintomas vestibulares, foram apenas recentemente padronizadas. Objetivo: Apresentar uma revisão dos principais assuntos da otoneurologia, inclusive conceitos, diagnóstico e tratamento da otoneurologia, definir a evidência científica atual para facilitar a tomada de decisões e demonstrar as áreas mais carentes de evidência para estimular novas pesquisas. Método: Este texto é fruto do I Fórum Brasileiro de Otoneurologia, que reuniu os principais pesquisadores brasileiros dessa área para uma revisão da literatura. Serão feitos três trabalhos de revisão a serem publicados. Este primeiro abordou as definições e as terapias, o segundo abordará as ferramentas diagnósticas e o terceiro definirá os principais diagnósticos. Cada autor fez um levantamento bibliográfico na base de dados da Lilacs, SciELO, Pubmed e Medline de um determinado assunto. O seu texto foi então submetido aos demais participantes do Fórum por 30 dias para análise. Um capítulo especial, da definição dos sintomas vestibulares, foi traduzido por serviço de tradução oficial e igualmente submetido às demais etapas do processo. Houve então uma reunião presencial em que todos os textos foram apresentados oralmente e houve uma discussão entre os participantes para a definição de um texto consensual para cada capítulo. Os textos consensuais foram então submetidos a uma revisão final por quatro professores de otoneurologia de três universidades brasileiras e, por fim, finalizado. A partir do texto completo, publicado no site da Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial, foi escrita esta versão-resumo como artigo de revisão. Resultado: O texto apresenta a tradução oficial para o português da definição dos sintomas vestibulares propostos pela Barany Society e agrupa as principais evidências científicas para cada um das principais terapias existentes para as doenças otoneurológicas. Conclusão: Este texto agrupou de forma racional os principais tópicos de conhecimento a respeito das definições e terapias da otoneurologia, permite ao leitor uma visão ampla da abordagem dos pacientes otoneurológicos baseada em evidências científicas e experiência nacional, que deverá auxiliá-lo na tomada de decisões clínicas, e mostra os assuntos mais carentes de evidência para estimular novos estudos.

Abstract Introduction: Neurotology is a rapidly expanding field of knowledge. The study of the vestibular system has advanced so much that even basic definitions, such as the meaning of vestibular symptoms, have only recently been standardized. Objective: To present a review of the main subjects of neurotology, including concepts, diagnosis and treatment of Neurotology, defining current scientific evidence to facilitate decision-making and to point out the most evidence-lacking areas to stimulate further new research. Methods: This text is the result of the I Brazilian Forum of Neurotology, which brought together the foremost Brazilian researchers in this area for a literature review. In all, there will be three review papers to be published. This first review will address definitions and therapies, the second one will address diagnostic tools, and the third will define the main diseases diagnoses. Each author performed a bibliographic search in the LILACS, SciELO, PubMed and MEDLINE databases on a given subject. The text was then submitted to the other Forum participants for a period of 30 days for analysis. A special chapter, on the definition of vestibular symptoms, was translated by an official translation service, and equally submitted to the other stages of the process. There was then a in-person meeting in which all the texts were orally presented, and there was a discussion among the participants to define a consensual text for each chapter. The consensual texts were then submitted to a final review by four professors of neurotology disciplines from three Brazilian universities and finally concluded. Based on the full text, available on the website of the Brazilian Association of Otorhinolaryngology and Cervical-Facial Surgery, this summary version was written as a review article. Result: The text presents the official translation into Portuguese of the definition of vestibular symptoms proposed by the Bárány Society and brings together the main scientific evidence for each of the main existing therapies for neurotological diseases. Conclusion: This text rationally grouped the main topics of knowledge regarding the definitions and therapies of Neurotology, allowing the reader a broad view of the approach of neurotological patients based on scientific evidence and national experience, which should assist them in clinical decision-making, and show the most evidence-lacking topics to stimulate further study.

https://doi.org/10.1016/j.bjorl.2019.11.002 2736 downloads

Thermal performance of nanoencapsulated phase change material in high molecular weight polystyrene
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Agner, Tamara

; Zimermann, Amadeo

; Machado, Fabricio

; Silveira Neto, Brenno Amaro da

; Araújo, Pedro Henrique Hermes de

; Sayer, Claudia

Polímeros

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2020, Volume 30 Nº 2 elocation e2020013

Resumo: En | Texto: En | PDF: En

Abstract A novel nanoencapsulation of n-hexadecane in high molecular weight polystyrene nanoparticles for thermal energy storage was carried out by miniemulsion polymerization using an iron-containing imidazolium-based ionic liquid (IL) as catalyst. The particle size, morphology, molecular weight, and thermal performance of nanoparticles containing the phase change material (PCM) were measured by dynamic light scattering, transmission electron microscopy, gel permeation chromatography, and differential scanning calorimetry, respectively. The nanoparticles were regular spherical, with narrow size distribution and particle size ranged from 138 nm to 158 nm. The enthalpy of melting for the nanoencapsulated PCM increased from 19 J/g to 72 J/g, as the content of n-hexadecane used increased from 20 wt% to 50 wt%. In addition, the nanoparticles showed thermal reversibility after 100 thermal cycles. The high molecular weights of the polymer, up to 1800 kDa, that could be reached with this IL may have contributed positively to this thermal behavior.

https://doi.org/10.1590/0104-1428.01320 637 downloads

10.

Análise bibliométrica de teses e dissertações brasileiras sobre travestilidade, transexualidade e saúde
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Lima, Rafael Rodolfo Tomaz de

; Flor, Taiana Brito Menêzes

; Araújo, Pedro Henrique de

; Noro, Luiz Roberto Augusto

Trabalho, Educação e Saúde

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2020, Volume 18 Nº 3 elocation e00301131

Resumo: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo Objetivou-se analisar o perfil das teses e dissertações sobre travestilidade, transexualidade e saúde nos Programas de Pós-Graduação stricto sensu do Brasil, por meio de um estudo fundamentado no referencial teórico-metodológico da análise bibliométrica. Os 65 trabalhos selecionados foram extraídos do Catálogo de Teses e Dissertações da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior e da Biblioteca Digital Brasileira de Teses e Dissertações. Constata-se que a maioria das produções são dissertações (82%) advindas dos Programas de Pós-Graduação em Saúde Coletiva (27%) e de Instituições Federais de Educação Superior (65%), localizadas nas regiões Sul e Sudeste (70%). A abordagem qualitativa foi a mais utilizada (82%) e o processo transexualizador no Sistema Único de Saúde foi o tema predominante (35%). O estudo revelou que as teses e dissertações brasileiras sobre a tríade travestilidade-transexualidade-saúde estão em ascensão nos últimos vinte anos, com diminuição no enfoque no adoecimento das travestis e de transexuais e aumento do foco nos aspectos sociais, organizacionais e políticos que interferem no acesso dessas pessoas aos serviços de saúde. Ainda assim, é preciso expandir as pesquisas sobre a temática para as demais regiões do país, impactando positivamente na produção científica e em um sistema de saúde mais equânime e universal.

Abstract The aim of this study was to analyze the profile of theses and dissertations on transvestite, transsexuality and health in the stricto sensu Post-Graduate Programs in Brazil, through a study based on the theoretical-methodological framework of bibliometric analysis. The 65 selected works were extracted from the Theses and Dissertations Catalog of the Coordination for the Improvement of Higher Education Personnel and the Brazilian Digital Library of Theses and Dissertations. It appears that most of the productions are dissertations (82%) coming from the Graduate Programs in Collective Health (27%) and from Federal Institutions of Higher Education (65%), located in the South and Southeast (70%). The qualitative approach was the most used (82%) and the transsexualizing process in the Unified Health System was the predominant theme (35%). The study revealed that Brazilian theses and dissertations on the transvestite-transsexuality-health triad have been on the rise in the last twenty years, with a decrease in the focus on the illness of transvestites and transsexuals and an increased focus on the social, organizational and political aspects that interfere in the access of these people to health services. Even so, it is necessary to expand research on the theme to other regions of the country, positively impacting scientific production and a more equitable and universal health system.

Resumen Se tuvo como objetivo analizar el perfil de las tesis y disertaciones sobre travestilidad, transexualidad y salud en los Programas de Posgrado stricto sensu de Brasil, por medio de un estudio fundamentado en lo referencial teórico-metodológico del análisis bibliométrico. Los 65 trabajos seleccionados se extrajeron del Catálogo de Tesis y Disertaciones de la Coordinación de Perfeccionamiento del Personal de Nivel Superior y de la Biblioteca Digital Brasileña de Tesis y Disertaciones. Se constata que la mayoría de las producciones son disertaciones (82%) que tienen lugar en los Programas de Posgrado en salud Colectiva (27%) y en Instituciones Federales de Educación Superior (65%), localizadas en las regiones Sur y Sureste (70%). El abordaje cualitativo fue el más utilizado (82%) y el proceso transexualizador en el Sistema Único de Salud fue el tema predominante (35%). El estudio reveló que las tesis y disertaciones brasileñas sobre la tríada travestilidad-transexualidad-salud están en ascensión en los últimos veinte años, y se nota una disminución en el enfoque sobre las enfermedades de las travestis y de transexuales y el aumento del enfoque en los aspectos sociales, organizacionales y políticos que interfieren en el acceso de esas personas a los servicios de salud. Aún así, es necesario expandir las investigaciones sobre la temática para las demás regiones del país, impactando positivamente en la producción científica y en un sistema de salud más ecuánime y universal.

https://doi.org/10.1590/1981-7746-sol00301 1276 downloads

11.

Bringing to light the molecular evolution of GUX genes in plants
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Gallinari, Rafael Henrique

; Coletta, Rafael Della

; Araújo, Pedro

; Menossi, Marcelo

; Nery, Mariana Freitas

Genetics and Molecular Biology

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2020, Volume 43 Nº 1 elocation e20180208

Resumo: En | Texto: En | PDF: En

Abstract Hemicellulose and cellulose are essential polysaccharides for plant development and major components of cell wall. They are also an important energy source for the production of ethanol from plant biomass, but their conversion to fermentable sugars is hindered by the complex structure of cell walls. The glucuronic acid substitution of xylan (GUX) enzymes attach glucuronic acid to xylan, a major component of hemicellulose, decreasing the efficiency of enzymes used for ethanol production. Since loss-of-function gux mutants of Arabidopsis thaliana enhance enzyme accessibility and cell wall digestion without adverse phenotypes, GUX genes are potential targets for genetically improving energy crops. However, comprehensive identification of GUX in important species and their evolutionary history are largely lacking. Here, we identified putative GUX proteins using hidden Markov model searches with the GT8 domain and a GUX-specific motif, and inferred the phylogenetic relationship of 18 species with Maximum likelihood and Bayesian approaches. Each species presented a variable number of GUX, and their evolution can be explained by a mixture of divergent, concerted and birth-and-death evolutionary models. This is the first broad insight into the evolution of GUX gene family in plants and will potentially guide genetic and functional studies in species used for biofuel production.

https://doi.org/10.1590/1678-4685-gmb-2018-0208 893 downloads

12.

Ultrasound assisted miniemulsion polymerization to prepare poly(urea-urethane) nanoparticles
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Polloni, André Eliezer

; Valério, Alexsandra

; Oliveira, Débora de

; Araújo, Pedro Henrique Hermes de

; Sayer, Claudia

Polímeros

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Jun 2018, Volume 28 Nº 2 Páginas 155 - 160

Resumo: En | Texto: En | PDF: En

Abstract Recently, the physical and chemical effects of ultrasound in polymeric materials synthesis have attracted great attention. This work presents the synthesis of novel polymeric materials by polymerization of isophorone diisocyanate with different polyols. Polymers were synthesized by step miniemulsion polymerizations, using ultrasound bath and thermostatic bath. The effects of ultrasound, temperature and polyol type were evaluated by Fourier transform infrared spectroscopy, gel permeation chromatography, dynamic light scattering and titrimetry. Polymerization under ultrasound bath showed that different reaction temperatures in the range between 50 °C and 80 °C directly influence the molecular weight of the polymers, urea/urethane formation and increase of diisocyanate consumption rate. In addition, different polyols used in polymerizations in miniemulsion had a significant effect on the characteristics of the resulting poly(urea-urethane) nanoparticles. Finally, ultrasound assisted polymerizations showed a faster diisocyanate consumption rate, but did not lead to enhanced molecular weights.

https://doi.org/10.1590/0104-1428.02517 1283 downloads

13.

DEVELOPMENT OF ANTIOXIDANT POLY(THIOETHER-ESTER) NANOPARTICLES
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Meneses, Alessandra Cristina de

; Machado, Thiago Ouriques

; Sayer, Claudia

; Araújo, Pedro Henrique Hermes

; Oliveira, Débora de

Brazilian Journal of Chemical Engineering

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Jun 2018, Volume 35 Nº 2 Páginas 691 - 698

Resumo: En | Texto: En | PDF: En

Abstract Poly(Thioether-Ester) nanoparticles synthetized via thiol-ene polymerization from a renewable castor oil monomer are produced by miniemulsion polymerization and emulsification/evaporation of pre-formed polymer. Its antioxidant activity, probably due to the oxidation of sulfide groups, is confirmed by 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical-scavenging and β-carotene/linoleic acid assays, with an amount of polymer required to reduce the initial concentration of DPPH· radicals by 50% of ~195 µg.ml-1 promoting hydrogen or electron exchange and the capability to prevent lipid peroxidation of ~55%. The results show a promising application in food packing.

https://doi.org/10.1590/0104-6632.20180352s20160605 1705 downloads

14.

CELLULASE IMMOBILIZATION ON POLY(METHYL METHACRYLATE) NANOPARTICLES BY MINIEMULSION POLYMERIZATION
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Simon, Patrícia

; Lima, Janaína S.

; Valério, Alexsandra

; Oliveira, Débora de

; Araújo, Pedro H.H.

; Sayer, Claudia

; Souza, Antônio Augusto U. de

; Souza, Selene M. A. Guelli U. de

Brazilian Journal of Chemical Engineering

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Jun 2018, Volume 35 Nº 2 Páginas 649 - 658

Resumo: En | Texto: En | PDF: En

Abstract Cellulases are efficient enzymes for the conversion of cellulose into glucose. Their use in immobilized form enables them to be reused in successive cycles in many biotechnological processes. Unlike conventional methods of immobilization by covalent bonding, in miniemulsion polymerization the immobilization of enzyme and the synthesis of polymer nanoparticles (support) occur simultaneously. Based on these aspects, the immobilization of cellulose on poly(methyl methacrylate) (PMMA) nanoparticles by miniemulsion polymerization was studied. The surfactant type (non-ionic and ionic) and latex pH showed great influence on cellulase activity. High activity values were obtained only when non-ionic surfactant (Lutensol AT50) and buffering agent (NaHCO3) were used simultaneously. MMA polymerization rate and final monomer conversion were not affected by the presence of cellulase. The maximum immobilization efficiency (60%) was obtained when 6 wt.% of cellulase was used and stable PMMA nanoparticles (133 nm) were obtained. The relative activity profile of immobilized cellulase, for pH as well as temperature, was similar to that reported for the free form. Immobilized enzyme keeps its activity throughout seven days when stored at 4 ºC and phosphate buffer pH 6.0. Based on the results obtained in this work, miniemulsion polymerization as a method for cellulase immobilization on PMMA nanoparticles showed to be a promising technique with high possibility of industrial application.

https://doi.org/10.1590/0104-6632.20180352s20160094 1465 downloads

15.

Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum
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LLERENA, JUAN P.P.

; ARAÚJO, PEDRO

; MAZZAFERA, PAULO

Anais da Academia Brasileira de Ciências

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Mar 2018, Volume 90 Nº 1 Páginas 509 - 519

Resumo: En | Texto: En | PDF: En

ABSTRACT Saccharum spontaneum has been used for the development of energy cane a crop aimed to be used for the production of second-generation ethanol, or lignocellulosic ethanol. Lignin is a main challenge in the conversion of cell wall sugars into ethanol. In our studies to isolate the genes the lignin biosynthesis in S. spontaneum we have had great difficulty in RT-PCR reactions. Thus, we evaluated the effectiveness of different additives in the amplification of these genes. While COMT and CCoAOMT genes did not need any additives for other genes there was no amplification (HCT, F5H, 4CL and CCR) or the yield was very low (CAD and C4H). The application of supplementary cDNA was enough to overcome the non-specificity and low yield for C4H and C3H, while the addition of 0.04% BSA + 2% formamide was effective to amplify 4CL, CCR, F5H and CCR. HCT was amplified only by addition of 0.04% BSA + 2% formamide + 0.1 M trehalose and amplification of PAL was possible with addition of 2% of DMSO. Besides optimization of expression assays, the results show that additives can act independently or synergistically.

https://doi.org/10.1590/0001-3765201820170250 2712 downloads

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