Physiological and anatomical features of Cd-tolerance in Pfaffia glomerata were examined by exposing plantlets to nutrient solutions with increasing Cd concentrations (0, 15, 45, and 90 µmol Cd L-1), and possible Cd-tolerance markers were established. Cd contents were found to be higher in roots than in shoots. According to the bio-concentration factor data, this species is effectively a Cd-hyperaccumulator, as previously attested. Cd induced the appearance of xeromorphic characteristics in leaves (decreased water potential, increased numbers and decreased stomata size) and increased root endodermis thickness. The enzymatic antioxidant systems of roots and leaves were differently affected by Cd. The coordinated activities of antioxidant enzymes were effective in reducing Cd-induced reactive oxygen species in plants, mainly in leaves. Root endodermis thickness, stomatal size and numbers, root superoxide dismutase, and guaiacol peroxidase, as well as leaf guaiacol peroxidase and catalase activities can all be considered Cd-tolerance markers in Pfaffia glomerata. Due to its high root Cd accumulation, Pfaffia glomerata may be useful in Cd-phytoextraction programs, however the pharmacological use of plants grown in the presence of Cd must be avoided.