Abstract Visceral Leishmaniasis is included among the neglected tropical diseases, being directly related to conditions of social vulnerability, in urban environments, dogs act as important reservoirs. The aim of the study was to evaluate the distribution of dogs, related risk factors and identify of volatile organic compounds from infected dogs. Peripheral blood samples from 72 dogs were collected for detection using the ELISA test, in addition to hair samples for analysis by GC-MS. Of the evaluated dogs, 13 (18.05%/72) were reactive for canine VL, seven in Aracaju and six in Propriá. Factors related to vegetation, age, place where the dog stays and free access to the street, were associated with a greater chance of the dog becoming infected. Fifty-three compounds were identified from ten canine hair samples, among which 2-butoxyethanol, benzaldehyde, decane, 2-phenylacetaldehyde, nonan-1-ol, 2-phenoxyethanol, nonanoic acid, 8-heptadecene and eicosane were found in seropositive dogs for leishmaniasis. The guardian's posture has been increasingly important, requiring more attention to the dog's health and actions aimed at environmental management in an attempt to reduce cases of canine VL in the state. Even though the identified VOCs have not been associated with leishmanial infection, it is of great use for understanding canine hair substances. diseases vulnerability environments reservoirs 7 test GCMS. GCMS GC MS. MS GC-MS 1 18.05%/72 180572 18 05 (18.05%/72 Propriá vegetation age street Fiftythree Fifty three 2butoxyethanol, 2butoxyethanol butoxyethanol 2 butoxyethanol, 2-butoxyethanol benzaldehyde decane 2phenylacetaldehyde, 2phenylacetaldehyde phenylacetaldehyde phenylacetaldehyde, 2-phenylacetaldehyde nonan1ol, nonan1ol nonanol nonan ol, ol nonan-1-ol 2phenoxyethanol, 2phenoxyethanol phenoxyethanol phenoxyethanol, 2-phenoxyethanol acid 8heptadecene heptadecene 8 leishmaniasis guardians guardian s state infection substances 18.05%/7 18057 0 (18.05%/7 18.05%/ 1805 (18.05%/ 18.05% 180 (18.05% 18.05 (18.05 18.0 (18.0 18. (18. (18 (1 (

Resumo A Leishmaniose Visceral está incluída entre as doenças tropicais negligenciadas, estando diretamente relacionada às condições de vulnerabilidade social, e sendo o cão e principal hospedeiro em ambientes urbanos. O objetivo do estudo foi avaliar a presença de cães com leishmaniose visceral em municípios do estado de Sergipe, Brasil, os fatores de risco relacionados e identificar compostos orgânicos voláteis de cães infectados. Amostras de sangue periférico de 72 cães foram coletadas para a detecção por meio do teste de ELISA, além de amostras de pelos para análise por GC-MS. Dos cães avaliados, 13 (18,05%/72) foram reagentes para LV canina, sendo sete em Aracaju e seis em Propriá. Fatores relacionados a vegetação, a idade, o local de permanência do cão, o livre acesso à rua, foram associados a maior chance do cão se infectar. Foram identificados 53 compostos a partir de dez amostras de pelo canino, dentre os quais o 2-butoxietanol, benzaldeído, decano, 2-fenilacetaldeído, nonan-1-ol, 2-fenoxietanol, ácido nonanóico, 8-heptadeceno e eicosano foram encontrados em cães soropositivos para leishmaniose. A postura do tutor tem se revelado cada vez mais importante, sendo necessária mais atenção à saúde do cão e ações direcionadas a gestão ambiental na tentativa de diminuir os casos de LV canina no estado. Mesmo que os COVs identificados não tenham sido associados à infecção leishmaniótica, são de grande utilidade para a compreensão de substâncias de pelos caninos. negligenciadas social urbanos Sergipe Brasil infectados 7 ELISA GCMS. GCMS GC MS. MS GC-MS avaliados 1 18,05%/72 180572 18 05 (18,05%/72 Propriá vegetação idade rua infectar 5 canino 2butoxietanol, 2butoxietanol butoxietanol 2 butoxietanol, 2-butoxietanol benzaldeído decano 2fenilacetaldeído, 2fenilacetaldeído fenilacetaldeído fenilacetaldeído, 2-fenilacetaldeído nonan1ol, nonan1ol nonanol nonan ol, ol nonan-1-ol 2fenoxietanol, 2fenoxietanol fenoxietanol fenoxietanol, 2-fenoxietanol nonanóico 8heptadeceno heptadeceno 8 importante leishmaniótica caninos 18,05%/7 18057 0 (18,05%/7 18,05%/ 1805 (18,05%/ 18,05% 180 (18,05% 18,05 (18,05 18,0 (18,0 18, (18, (18 (1 (

Accidental transmission of Chagas' disease to man by blood transfusion is a serious problem in Latin-America. This paper describes the testing of several synthetic, semi-synthetic, and natural compounds for their activity against blood trypomastigotes in vitro at 4-C. The compounds embody several types of chemical structures: benzoquinone, naphthoquinone, anthracenequinone, phenanthrenequinone, imidazole, piperazine, quinoline, xanthene, and simple benzenic and naphthalenic derivates. Some of them are for the first time tested against Trypanosoma cruzi. The toxic effect these compounds on this parasite was done by two quite distinct sets of experiments. In one set, the compounds were added to infected blood as ethanolic solution. In this situation the most active one was a furan-1, 2-naphthoquinone, in the same range as gentian violet, a new fact to be considered in the assessment of structure-activity relationships in this class of compounds. In other set, we tentatively evaluated the biological activity of water insoluble compounds by adding them in a pure form without solvent into infected blood. In this way some appear to be very active and it was postulated that the effectiveness of such compounds must result from interactions between them and specific blood components.