ABSTRACT An increasing amount of sunlight is available to sunken greenhouses located in northern China, but it remains unclear how the indoor temperature changes at different heights and at the north and south ends of the structure. To study how the temperature in a single indoor section, at multiple points, varies in a sunken solar greenhouse, 10 measurement lines were arranged in a section 30 m from the west wall. Each line was equipped with a corresponding WT-59 wireless sensor. Temperature and humidity data were collected from 8 March 2019 to 26 December 2019. The following results were obtained. (1) The indoor temperature variation in a day could be divided into four stages: from 0:00 to 7:30 h, the temperature at all measurement points decreased linearly; from 7:30 to 14:30 h, the temperature at all measurement points increased curvilinearly; from 14:30 to 18:00, the temperature at all measurement points decreased curvilinearly; and from 18:30 to 24:00, the temperature at all measurements points decreased linearly. (2) The maximum temperature on sunny days in the greenhouse was higher than that on cloudy days and snowy days, but the maximum temperature on cloudy days and snowy days in the greenhouse occurred 3 h earlier than on sunny days. (3) The lowest average temperature of a sunken solar greenhouse over 3–6 months was 7.5ଌ, without heating. The research results provide theoretical and reference data on how the temperature changes in the interior of a solar greenhouse and inform about the three-dimensional planting height and ventilation times.

For immobilization of puerarin glycosidase from Microbacterium oxydans CGMCC 1788 on DEAE-52 cellulose, the optimal amount of enzyme protein was 12 mg protein: 1 g DEAE-52 cellulose; the optimal pH was 6.5; and the optimal immobilization time was 6 hr. The specific activity of immobilized enzyme was 36.67 mU.g-1 carrier with an immobilization yield of 98.87% and an enzyme recovery yield of 92.43%. The molar transformation rates of puerarin by immobilized enzyme and by the relative bacterial cell amount equal to the same amount of enzyme were 53.3% and 2.2%, respectively, after 1 hr of transformation. The former molar transformation rate, which was similar to that for free enzyme, was more than 24-fold greater than the latter. The immobilized puerarin glycosidase showed improved enzymatic properties and stability. The immobilized puerarin glycosidase retained 88% of its initial activity after being reused 10 times.