RESUMO Introdução O Comitê Central do CPC e o Conselho de Estado destacaram que, atualmente, os indicadores básicos da saúde física dos jovens estudantes têm diminuído de forma completa. Essa circunstância tornou a saúde física dos adolescentes e a melhoria da qualidade dos jovens atletas um ponto-chave na educação física direcionada aos adolescentes. Objetivo Investigar o efeito do treinamento intermitente de alta intensidade em jovens atletas. Métodos 24 adolescentes realizaram 10 treinos intermitentes de alta intensidade, com duração de um mês, todos os índices pré e pós-treino dos atletas foram coletados e comparados. Resultados Os resultados do exame físico de atletas do sexo masculino e feminino melhoraram em certa medida (a pressão arterial dos atletas flutuou durante as 10 sessões de treinamento, aumentando ligeiramente, e a variabilidade da frequência cardíaca aumentou consideravelmente (P < 0,05). Conclusão O treinamento intermitente de alta intensidade tem um bom efeito na promoção da saúde nos jovens atletas, podendo melhorar efetivamente sua função física e desempenho competitivo. Professores de educação física e atletas estudantes devem realizar treinamentos relevantes. Além disso, esse método de treinamento também se encaixa na situação atual da vida de aprendizagem dos alunos do ensino médio. Nível de evidência II; Estudos terapêuticos - investigação dos desfechos do tratamento.

ABSTRACT Introduction The CPC Central Committee and the State Council have pointed out that currently, the basic indicators of young students’ physical health have completely declined. This circumstance has made adolescent physical health and improving the quality of young athletes a key issue in physical education targeting adolescents. Objective Investigate the effect of high-intensity intermittent training on young athletes. Methods 24 adolescents performed 10 intermittent high-intensity training for one month, and all athletes’ pre and post-training indexes were collected and compared. Results The physical examination results of male and female athletes improved to some extent (athletes’ blood pressure fluctuated during the 10 training sessions, increasing slightly, and heart rate variability increased considerably (P < 0.05). Conclusion High-intensity intermittent training has a good effect on health promotion in young athletes, and can effectively improve their physical function and competitive performance. Physical education teachers and student-athletes should conduct relevant training. In addition, this training method also fits the current learning life situation of high school students. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Introducción El Comité Central del CPC y el Consejo de Estado han puesto de relieve que en la actualidad los indicadores básicos de salud física de los jóvenes estudiantes han disminuido completamente. Esta circunstancia hizo que la salud física de los adolescentes y la mejora de la calidad de los jóvenes deportistas fuera un punto clave en la educación física dirigida a los adolescentes. Objetivo Investigar el efecto del entrenamiento intermitente de alta intensidad en atletas jóvenes. Métodos 24 adolescentes realizaron 10 sesiones de entrenamiento intermitente de alta intensidad de un mes de duración, se recogieron y compararon los índices de todos los atletas antes y después del entrenamiento. Resultados Los resultados de la exploración física de los atletas masculinos y femeninos mejoraron en cierta medida (la presión arterial de los atletas fluctuó durante las 10 sesiones de entrenamiento, aumentando ligeramente, y la variabilidad de la frecuencia cardíaca aumentó considerablemente (P < 0,05). Conclusión El entrenamiento intermitente de alta intensidad tiene un buen efecto en la promoción de la salud en los atletas jóvenes, y puede mejorar eficazmente su función física y su rendimiento competitivo. Los profesores de educación física y los alumnos deportistas deben realizar la formación pertinente. Además, este método de formación también se ajusta a la situación actual de la vida de aprendizaje de los estudiantes de secundaria. Nivel de evidencia II; Estudios terapêuticos - investigación de los resultados del tratamiento.

ABSTRACT Background: With the reduction of global forest resources and the intensification of environmental problems, it is particularly important to seek new solutions for forest resource management. This paper proposes a forest resource management system based on blockchain technology to realize data security sharing and traceability of forest ownership. Through drone on-site verification and blockchain technology, management credibility and efficiency are improved and costs are reduced. The system provides innovative solutions in terms of data credibility, cooperation mechanism, intelligent decision-making, sustainable development, etc., and defines the future development direction. Background problems ownership onsite site reduced mechanism decisionmaking, decisionmaking decision making, making decision-making etc etc. direction

SUMMARY OBJECTIVE: This study aimed to investigate the factors associated with behavioral problems in children with congenital pseudarthrosis of the tibia. METHODS: Random sampling is utilized to obtain a sample of 90 patients. The behavioral problems of the patients are detected by Achenbach Children's Behavior Scale. Parental emotional problems are investigated by the Self-Rating Depression Scale and Self-Rating Anxiety Scale. RESULTS: The results demonstrate that the detection rate of behavioral problems in children with congenital pseudarthrosis of the tibia is 53.3% (48/90). Among these behavioral problems, an abnormal rate is higher in the four dimensions: thinking, violation of discipline, social interaction, and aggression. The anxiety and depression scores of caregivers are statistically higher in the abnormal group than in the normal group. The results of the multivariate analysis show that the anxiety degree of the parents had a significant impact on the behavior of the children. CONCLUSIONS: Children with congenital pseudarthrosis of the tibia are facing the issues of high rates of behavioral problems. Parents of children with congenital pseudarthrosis of the tibia had higher levels of anxiety and depression than parents of normal children. The anxiety and depressive state of mind of parents or caregivers had a significant impact on the behavior of children with congenital pseudarthrosis of the tibia.

Abstract This study aimed to uncover transcription factors that regulate super-enhancers involved in glucose metabolism reprogramming in poorly differentiated thyroid carcinoma (PDTC). TCA cycle and pyruvate metabolism were significantly enriched in PDTC. Differentially expressed genes in PDTC vs. normal control tissues were located in key steps in TCA cycle and pyruvate metabolism. A total of 23 upregulated genes localized in TCA cycle and pyruvate metabolism were identified as super-enhancer-controlled genes. Transcription factor analysis of these 23 super-enhancer-controlled genes related to glucose metabolism was performed, and 20 transcription factors were obtained, of which KLF12, ZNF281 and RELA had a significant prognostic impact. Regulatory network of KLF12, ZNF281 and RELA controlled the expression of these four prognostic target genes (LDHA, ACLY, ME2 and IDH2). In vitro validation showed that silencing of KLF12, ZNF281 and RELA suppressed proliferation, glucose uptake, lactate production and ATP level, but increased ADP/ATP ratio in PDTC cells. In conclusion, KLF12, ZNF281 and RELA were identified as the key transcription factors that regulate super-enhancer-controlled genes related to glucose metabolism in PDTC. Our findings contribute to a deeper understanding of the regulatory mechanisms associated with glucose metabolism in PDTC, and advance the theoretical development of PDTC-targeted therapies.

RESUMO Objetivo: Avaliar a precisão e o efeito da matriz de guia de broca para colocação de parafuso pedicular em escoliose grave. Método: Oito pacientes com escoliose rígida foram selecionados, sendo cinco homens e três mulheres na faixa etária de nove a 23 anos. Foi realizada TC tridimensional da coluna, gravada no formato DICOM. A matriz multinível foi desenhada pelo software Mimics e fabricada de acordo com a parte da deformidade mais grave. A matriz da broca foi colocada na superfície vertebral correspondente. Os parafusos pediculares foram cuidadosamente inseridos ao longo da trajetória da matriz. No pós-operatório, as posições dos parafusos pediculares foram avaliadas por TC e classificadas para validação. Resultados: Não ocorreu nenhuma lesão da medula espinhal nem lesão de nervos. Todos os pacientes tiveram desfechos satisfatórios. As anormalidades e as medidas observadas durante a operação foram as mesmas encontradas no pré-operatório. A posição dos parafusos pediculares foi precisa, de acordo com a radiografia e a TC pós-operatórias. A taxa de correção de escoliose foi de 60%. Em comparação com os controles, o tempo de cirurgia, a perda de sangue e a radiação foram significantemente menores. Conclusão: Com a aplicação da matriz multinível, a colocação dos parafusos pediculares mostra alta precisão na escoliose, com menor tempo cirúrgico, menos perda de sangue e menor exposição à radiação. Nível de Evidência III, Estudo Retrospectivo Comparativo.

ABSTRACT Objective: To evaluate the accuracy and the effect of drill guide template for pedicle screw placement in severe scoliosis. Method: Eight patients with rigid scoliosis were enrolled, five males and three females, ranging from nine to 23 years old. A three-dimensional CT scan of the spine was performed and saved as a DICOM file type. The multi-level template was designed by Mimics software and manufactured according to the part of the most severe deformity. The drill template was placed on the corresponding vertebral surface. Pedicle screws were carefully inserted across the trajectory of the template. Postoperatively, the positions of the pedicle screws were evaluated by CT scan and graded for validation. Results: No spinal cord injury or nerve damage occurred. All patients had satisfactory outcomes. The abnormalities and the measures observed during operation were the same as those found in the preoperative period. The position of the pedicle screws was accurate, according to the postoperative X-ray and CT scan. The rate of scoliosis correction was 60%. Compared with controls, surgery time, blood loss and radiation were significantly lower. Conclusion: With the application of multi-level template, the placement of pedicle screws shows high accuracy in scoliosis with shorter surgical time, less blood loss and less radiation exposure. Level of Evidence III, Retrospective Comparative Study.

ABSTRACT A new C21 steroidal glycoside, paniculatumoside G, together with neocynapanogenin C isolated for the first time from the natural source and two known compounds were isolated and characterized from the roots and rhizomes of Cynanchum paniculatum (Bunge) Kitag. ex H.Hara, Apocynaceae, a commonly used Traditional Chinese Medicine. On the basis of spectroscopic analysis, including HR-ESI-MS, 1D and 2D NMR spectral data, the structure of the new C21 steroidal glycoside was elucidated as neocynapanogenin H 3-O-β-D-oleandropyranoside.

Abstract The D-genome progenitor of wheat (Triticum aestivum), Aegilops tauschii, possesses numerous genes for resistance to abiotic stresses, including drought. Therefore, information on the genetic architecture of A. tauschii can aid the development of drought-resistant wheat varieties. Here, we evaluated 13 traits in 373 A. tauschii accessions grown under normal and polyethylene glycol-simulated drought stress conditions and performed a genome-wide association study using 7,185 single nucleotide polymorphism (SNP) markers. We identified 208 and 28 SNPs associated with all traits using the general linear model and mixed linear model, respectively, while both models detected 25 significant SNPs with genome-wide distribution. Public database searches revealed several candidate/flanking genes related to drought resistance that were grouped into three categories according to the type of encoded protein (enzyme, storage protein, and drought-induced protein). This study provided essential information for SNPs and genes related to drought resistance in A. tauschii and wheat, and represents a foundation for breeding drought-resistant wheat cultivars using marker-assisted selection.